... Mice can be easily manipulated with tools like CRISPR to make transgenic lines. The fruit fly (Drosophila...

...revolution in genome editing methods (most notably CRISPR/Cas mediated HDR) enables the tagging of endogenous...

... interactions Learn how biotin can be used with CRISPR Additional resources Idtdna educational pages...

... instance, the popular genome-engineering tool, CRISPR, may one day make techniques used to generate transgenic...

...detect endogenous tagged genes generated through CRISPR/Cas9 knock-in. Finally, NLuc is very inviting for...

...Genome-wide programmable transcriptional memory by CRISPR-based epigenome editing. Cell, 184(9), 2503–2519... Gilbert Labs have previously deposited their CRISPRoff system — a programmable epigenetic editor consisting...deposited with Addgene. These variants, including a “miniCRISPR” version, are much smaller proteins than SpyCas9...

...also like... Addgene's Genome Engineering Guide CRISPR Plasmids Zinc Finger Kits TALENs are important ...

...vectors fantastic options for delivering shRNA, CRISPR/Cas9 components, and fluorescent sensors. Retroviral...

...Knockin Plasmids to Disrupt Genes in Mammalian Cells CRISPR Methods for Bacterial Genome Engineering Resources...

...electrical activity to gene expression, and even CRISPR based gene editing. But, how do you decide which...

... of other technologies such as optogenetics and CRISPR/Cas9 will help immunologists create even better...

...sequence cloned into it. gRNAs are used for directing CRISPR/Cas9 to the appropriate genomic location, while...

...Plasmids 101-Viral Vector Elements Blog: AAV use in CRISPR Viral Vectors 101 eBook Packaging Plasmids AAV ...

...delivered: fluorescent proteins, optogenetic sensors, CRISPR elements, etc... *These are only starting recommendations...

... 96 parts including terminators and promoters, CRISPRi parts, selectable markers, transformation acceptor...promoters, and inhibited gene expression using CRISPRi. The kit functions in the model cyanobacterium ...

...Autophagy Sensor Read about Aptamer Fluorophores Use CRISPRainbow for Genome Visualization Resources on Addgene.org...

...Parkinson's Frank Soldner 180437 Px330-EGFP-LRRK2-CRISPR/Cas9 LRRK2 U6 Parkinson's Frank Soldner 180439 ...Familial British dementia Jan Johansson 138347 lentiCRISPR v2-sgDnmt1_1 DNMT1 U6 Hereditary sensory neuropathy...neuropathy type IE Yi Zhang 138348 lentiCRISPR v2-sgDnmt1_2 DNMT1 U6 Hereditary sensory neuropathy type ...LRRK2 His T7 Parkinson's Suzanne Pfeffer 186025 lentiCRISPR v2-sgCOQ2 COQ2 U6 Multiple system atrophy Boyi...pET-Duet_OPTN-thrombin-GST OPTN GST T7 ALS Sascha Martens 218527 plentiCRISPR-v2 Puro EIF2AK2/PKR_sgRNA EIF2AK2 U6 VWM disease...

... and Resources You May Also Like: Viral Service CRISPR Fluorescent Proteins Cancer Research Plasmids Gene...