Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools
Type
Blog Post
...library was used to transduce T helper type 2 (Th2) cells from mice that constitutively express Cas9 and...genome-wide interrogation of the genes required for cellular processes. For a successful screen, it is necessary... on the method used to introduce the gRNAs into cells. Most CRISPR pooled libraries have been created ...the chance of transducing a only a single gRNA per cell. The viral DNA containing the gRNA then integrates...next-generation sequencing of the genomic DNA. However, some cell types are poorly transduced by lentivirus, and thus...CRISPR libraries was not possible.
Primary murine T cells cannot be transduced by lentivirus and have been...CRISPR pooled library can be used for screening of cells that are refractory to lentiviral transduction, ...