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  1. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...Yoshimi, K., Okuzaki, Y., Gee, P., Kunihiro, Y., Sonpho, E., Xu, H., Sasakawa, N., Naito, Y., Nakada, S...INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons Additional resources on Addgene.org CRISPR ...

  4. Plasmids 101: Degron Tags

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    Blog Post
    ...Thankfully, this is a hot topic with many resources! In Bondeson et al, they review all the tags discussed here...informed choice! References and Resources References Bondeson, D.P., Mullin-Bernstein, Z., Oliver, S. et al....

  6. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...these therapeutics. Jesse Bloom’s lab at the Fred Hutchinson Cancer Research Center developed a pooled plasmid...identified.     New from the viral service By Jason Nasse We regularly add new viral aliquots from our...

  8. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    ...to capitalize on your experience. No matter the reason for attending, your preparation and day-of game...hear or understand what we are being asked. I am personally guilty of this, and have many times answered ...

  10. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...consider performing PEG precipitation. Instead of sonicating the cell pellet, I lyse the cells with this AAV... Stone and Harshana de Silva Feelixge at Fred Hutchinson Cancer Center for teaching me about AAV production...

  11. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...Byron Rubin, a crystallographer working with Jane Richardson, provided an initial solution to this problem...are interested in experiencing Cas9 up close and personal, visit the 3D Molecular Designs web site. Or, ...

  15. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ... figure by figure organization with a different person responsible for each figure. Others still are more... avenues including casual request via email, in person, or instant messaging but we as scientists often...

  16. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    ...philosophy of  “work hard, play hard.” Part of the reason we sincerely like spending time together is that...work” all the time. She was a wise lady for many reasons, but this saying is especially true. If your event...

  17. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...Pk1] in a western blot assay. Image reused from Anderson et al., 2024 under CC BY license.    Find ...Find Anti-V5 [IPI-SV5-Pk1] Antibody here!   Anderson, Z., Zhu, H., Riedel, T., Moshinsky, D., Meijers, R...

  18. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...proteins, and modifications to the pegRNA. The Britt Adamson Lab recently used a CRISPRi screen to identify ...useful materials for research into Alzheimer’s, Parkinson’s, ALS, and Huntington’s Disease, including plasmids...

  19. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    ... Quick tips for writing a good cover letter Personalize your cover letter to the job and job requirements...Soriano-Carot in September 2016 and was updated by Rachel Leeson in May 2024.     Additional Resources on the Addgene...

  20. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...Langan, E., Siebert, S., Li, J., Greiner, D., & Anderson, D. (2015). Neutrophil Responses to Sterile Implant...  Rachel Leeson...
Showing: 421 - 440 of 718 results