... from your sequencing runs.   When you run a restriction digest on a gel you always include proper controls...primer at least 50bp upstream of the sequence of interest. 5. Use a silica spin column for purification ...

...this plasmid has a U6 promoter followed by BbsI restriction sites into which you can clone your 20 nucleotide...can use to express both SpCas9 and your gRNA of interest in a variety of mammalian cell lines. Alternative...

...to be used as research tools is particularly interesting. Although humans have known about bioluminescent...deciding which one is right for you, so if you’re interested in learning more about fluorescent proteins, ...

...Ubiquitin - One particular gene we saw increased interest in from 2008 to 2009 was ubiquitin. The most requested...next Top 10 list. 10 years of plasmid sharing  Interested in more facts about Addgene's 10 years of plasmid...

...can be applied non-invasively, its use is not restricted to cultured cells but extends to freely moving...plasmids have clear application to anyone with an interest in neuroscience, their use is by no means limited...

...discussed further in this informative post. Interestingly, this technology may make some uses of Site ... Software Engineer Kris Shamloo wrote a very interesting post about how we develop our in house lab management...

...screen for pathways that confer any phenotype of interest, not just drug resistance,” he said. “It could...contacted by investigators all over the world who are interested to use our libraries to do things - sometimes...

...depending on the sample type and the protein of interest. You may need to try a variety of fixation methods...transfected with a plasmid to express the protein of interest, and a negative control sample such as cells that... that do not express the protein of interest....

...characteristics and ectopic reporter assays. We have been interested in exploring the function of non-coding regulatory...frameshift mutations. However these may require restriction fragment length polymorphism analysis, allele-specific...

...of AAV plasmids will have technical or legal restrictions that make them ineligible for use in this service...consideration will have a banner on their material page. Interested scientists can expand the banner to select their...

...double-stranded DNA break at the genomic loci of interest and on either side of the cargo (ex: fluorescent...the double-stranded break in the genomic loci of interest. As the universal sgRNA is not predicted to target...

...Of course, if you’re interested in using IBEX, you’re probably also very interested in having high-quality...

...specifically isolate DNA that is bound by a protein of interest. First, nuclei from tissue or cell culture are...with a primary antibody against the protein of interest. The Protein A fused MNase is then added and Protein...

...works to meet the individual needs of schools interested in using eScience Labs as part of their curriculum...Learn more about alternative science careers here Interested in creative science education tools? Check out...

...conditions without necessarily modifying the gene of interest in any way. For example, if green fluorescent ...situations. Tissue-specific genes – the genes of interest may not be expressed in the tissue/cell line you...

...between recurring meetings, you should share interesting results or ask questions to your collaborators...microscopes and allowed me to investigate my protein of interest in a French clinical cohort, neither of which ...

...activated in response to a ligand binding a GPCR of interest. How does TRUPATH work? The TRUPATH collection...transfected with plasmids expressing the GPCR of interest, a Gα-Rluc8 plasmid, and the appropriate Gγ-GFP2...

...added to the N or C terminus of your protein of interest. Many of these tags also have commercially available...makes it amenable to being left on your protein of interest, especially if downstream antibody detection is...

...discovered? The discovery of GeoCas9 started with an interesting question: why engineer a new Cas9 when you can...CRISPR Methods for Bacterial Genome Engineering   Interested in using CRISPR/Cas9 RNPs? Check out this post...

...allow you to test the function of Your Gene Of Interest (YGOI) in a controlled environment under various... downstream of the open reading frame (ORF) of interest –  try pRS plasmid shuttle vectors. You may prefer...