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  2. Advice for Scientists Starting a Lab

    Type
    Blog Post
    ...goals. These first hires will set the tone and atmosphere that future hires will look up to." She also ... "Running Your Lab" booklet. What has been the most surprising thing about becoming a PI? Tom Ellis ...

  3. Viral Vectors 101: Gamma-Retroviral Packaging Systems

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    Blog Post
    ...integrated into the host genome upon viral transduction. Most transfer plasmids available from Addgene were derived...can be. Plasmids that encode SIN γ-retrovirus lack most of the U3 region of the 3’ LTR and are primarily...

  4. Viral Vectors 101: Viruses as Biological Tools

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    Blog Post
    ...nuclear envelope, which occurs during mitosis. The most well-known retrovirus is HIV. Lentiviruses are ... Gene Therapy with AAV Figure out How to Get the Most from Your Lentiviral Transduction Additional Resources...

  5. Sharing is Caring: But How to Distribute Open Hardware?

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    Blog Post
    ...the light microscope has been a powerful tool in almost all disciplines. In biology, innovative new imaging...Since I started my PhD, I have been convinced that most microscopy techniques can be replicated with commercially...

  6. Multiplex Genome Editing with CRISPR-Cpf1

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    Blog Post
    ...Overall, these approaches have two main drawbacks: 1) Most rely on transfection of more than one vector to ...lentiviral transduction, and AAV transduction.  In most of Zetsche et al’s experiments, a single vector ...

  7. Plasmids 101: Inducible Promoters

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    Blog Post
    ...promoters Chemically regulated promoters are among the most common inducible promoters. The positive inducible... agents to allow promoter activation. One of the most commonly used prokaryotic promoters is the negative...

  8. A Quick Guide to a Career in Software Product Management

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    Blog Post
    .... While some developers had science backgrounds, most did not. It was my job to translate complex lab ...front-end and back-end coding frameworks to use. I mostly sit and listen to stay informed on the details,...

  11. Behind the scenes: Addgene’s new search engine and more

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    Blog Post
    ...educational resources. Filters We identified the most useful kinds of information per resource category... details that make our new search engine unique. Most importantly, with this new implementation, we laid...

  13. Building and Validating Recombinant Antibodies: A Case Study

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    Blog Post
    ...available to the research community.    Validation For most cloning projects, the next step would be to validate...digest of the PCR products prior to cloning, which in most cases left the functional light chain intact, the...

  14. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

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    Blog Post
    ...intrigued by the tRNA processing machinery present in almost every living organism. Precursor, or pre-tRNAs ...levels of gRNA expression. Since tRNAs are among the most abundant molecules in a cell, this system is clearly...

  16. Kiran Musunuru on the Newest TALEN Genome-Editing System

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    Blog Post
    ... a homologous template provided by the sister chromosome. As cells take those two ends and put them back... a technology that was difficult for all but the most experienced users and puts it in the hands of pretty...

  17. Antibodies 101: Conjugation

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    Blog Post
    ...conjugate class than fluorophores are enzymes. The most common enzyme conjugates are Horseradish Peroxidase...be achieved through several different mechanisms. Most take advantage of chemical groups intrinsically ...

  18. To Each HIS Own

    Type
    Blog Post
    ...isolate proteins, this blog will focus on one of the most common, purification based on the polyhistidine ...containing two or more adjacent histidines are present in most production systems and can bind and co-elute with...

  19. CRISPR 101: Cas9 vs. The Other Cas(s)

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    Blog Post
    ...The shredder What sets Cas3 apart from Cas9 and almost all other Cas enzymes is its method of DNA cleavage...abilities   Choosing a Cas While Cas9 may be the most adaptable Cas currently available, the other Cas...

  20. Gendered Innovations: Why Does Sex of the Cell Matter?

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    Blog Post
    ... 145,000 more quality-adjusted life years. While most of the results were positive, the analysis did find...et al. provide a table indicating the sex of the most commonly used cell lines appearing in American Journal...
Showing: 421 - 440 of 759 results