...early I’m fortunate to live in a town with a good public school system that has made strong efforts in the...simplistic and I would insult the students. Too complicated and I would quickly lose their interest. I won...opinion, it was more important to communicate the applications of CRISPR over the details of how it worked....examples I used in my presentation had only become public in the previous two weeks, which demonstrated to...

...describe this technology and some of its potential applications? Fujii: We generated a series of retroviral ...the peer-review system of journals and grant applications, for example. However, at the same time, it'.... So, young researchers need to publish good publications in a short period of time and may need to do...available. Visit Hodaka Fujii lab's Addgene page and click the "Subscribe to Alerts" link in the Addgene Alerts...

...have real conversations with other scientists. Click Here to Visit Joanne’s AMA I've heard of Reddit,...Anything” was appealing, but also scary. I do a lot of public speaking where I enjoy communicating face to face...posters (this advice included leaving out the term “implicit bias” which is apparently a troll trigger phrase...recommend taking every opportunity you get to speak publically about your work (you can find my tips for a perfect...

...it down until you have something tangible and applicable to your situation.   Thinking Outside the Box...give people more work. Identify your enemies In Felicia Day’s book Embrace your Weird, Day identifies nine...for your idea. Our product team has implemented a policy of never rejecting an idea simply because it's ...innovative group. Our entire company benefits from their policy! Perfectionism When we reach for perfection, our...

..., mediates protein trans-splicing, similar to an intron in pre-mRNA splicing. In 2016, Fine et al developed...finding smaller Cas9 orthologs works for some applications, it doesn’t eliminate the need to deliver larger...Brown MT, Deshmukh H, Kemp ML, Bao G (2015) Trans-spliced Cas9 allows cleavage of HBB and CCR5 genes in human...

...hour. Find the SHERLOCK plasmids at Addgene! Applications of the SHERLOCK detection system The Zhang lab...portability. This method can be used for an array of applications including clinical diagnostics (e.g. pathogen...patient samples. Find the DETECTR plasmids here! Applications of DETECTR in diagnostics DETECTR allows for...an array of molecular and clinical diagnostic applications. The company Mammoth Biosciences has been started...

...Institute, Public Domain, National Institutes of Health, Wikipedia user Aimecie~commonswiki, Public Domain...let alone the human cost. Here are just a few publications which have since been retracted due to cell ...profiles are available for all the major cell lines on public databases. Image attributions (from top to bottom...

... of a mystical "Force," this quote is equally applicable to many microscopy experiments with fluorescent...How can one ensure that an FP fusion with a non-cytosolic protein functions properly? We suggest starting... non-optimized parental proteins. Our recent publication illustrated the quantitative increase in fluorescent...and Jack Griffith, her research focuses on the replication machinery of Kaposi’s sarcoma-associated herpesvirus...

...resistance gene and the origin of replication are necessary to replicate plasmids and make sure the cells...chicken beta actin promoter, and rabbit beta-globin splice acceptor. TRE General expression mRNA Tetracycline... General expression Promoter of the arabinose metabolic operon Inducible by arabinose and repressed catabolite...

...information on NIR FPs, their properties and applications. At the end, there is a Table that summarizes...their enhanced versions named emiRFPs in your applications.  There is a whole set of spectrally distinct... about the characteristics, performance, and applications of near-infrared calcium indicators in this ...of Medicine. Her expertise is development and applications of fluorescent proteins, genetically encoded...

...cell types, and research applications. Labeling neurons with Brainbow The Lichtman group developed the original... Brainbow 3.1 and 3.2 (described in the same publication) are unique because by default, they are not ...level of these XFP is not strong enough for all applications. To resolve this, similar systems have been ...

...Yizhar, and Karl Deisseroth. "The development and application of optogenetics." Annual review of neuroscience...SSharma, Suveena, and Jinfang Zhu. "Immunologic applications of conditional gene modification technology ...Thompson. "Overview on research and clinical applications of optogenetics." Current protocols in pharmacology... J. Simon, et al. "Silencing neurons: tools, applications, and experimental constraints." Neuron 95.3 ...

...resource for anyone looking to use antibody-based applications in their research.  Like all our eBooks, the...through the process. If you’re confident in your application and protocols, but can’t quite seem to find an...for Your Experiment.” Whatever your antibody application question, the Antibody 101 eBook is a great place...

... required for a growing organism to make the complicated transition from a clump of cells to a fully differentiated...track where GFP labelled B cells were in mounted slices of these organs. These GFP labelled cells could...been created to fate map Langerhans cells and follicular dendritic cells within the skin and lymph node...of antiviral CD8+ T cells in the peripheral interfollicular region of lymph nodes." Nature immunology 9.2...

...up. Applications of photoactivatable fluorescent proteins Some of the most important applications for ...level of background fluorescence. For any PA-FP application, the brighter the fluorescence the better. More...photostable green and red states. Beyond these imaging applications PA-FPs have been developed for use in yeast,...

...sample. Therefore, when a large number of optical slices are acquired all fluorophores throughout the sample...manufacturers have similar information References 1. Lichtman JW, Conchello JA. Fluorescence microscopy. Nature...2005). PubMed PMID: 16299476. 2. Conchello JA, Lichtman JW. Optical sectioning microscopy. Nature methods...2005). PubMed PMID: 16299478. 9. Richardson DS, Lichtman JW. Clarifying Tissue Clearing. Cell 162, 246-...

...cellular impediments. FRET has been used in many applications, including drug discovery by big pharma (i.e...innovation, valuable commodities in any NIH grant application! Using FRET, you may gain a new structural understanding...experiments. Donor Acceptor Tag R0 (Å)a Range (Å)b Applications CFP   Lumio Green Tc: CCPGCC 50 40-72 Intact...discovery–a review of theoretical aspects and recent applications." Current Chemical Genomics and Translational...

... approximately three weeks faster, as no license, or license fee, will be required. If you have any questions...Callaway   For researchers in countries with applicable export control exceptions for rabies and VSV-...

...specific subjects in the interview. Happy Listening! Click here to listen! Podcast breakdown: (Time : Topic... PMCID: PMC4096992. Find plasmids from this publication at Addgene. 2. Tang, Jonathan C.Y., et al. “...PubMed PMID: 26258682. Find plasmids from this publication at Addgene. Resources on the Addgene Blog Read...

...expected when we originally sent out the call for applications. Cervical cancer screening in Ghana Gladys is...Papillomavirus (HPV). As Gladys wrote in her application, “Cervical Cancer is the leading cause of cancer...Seeding Labs Plasmid Grant to a third round of applicants at the start of 2017 and would like to thank ...