...will make those new plasmids available through Addgene, and they hope others will do the same. “This isn...Resources Addgene's Synthetic Biology Overview More Synbio Blog Posts ... Kendall Morgan...

...vectors to generate a kit which is now available at Addgene. “We called it cloning by mail,” Wood says, and...PMCID: PMC4353587. Additional Resources on the Addgene Blog Learn How the Clon Tracer Library Has Been...growth, survival, and differentiation.” https://www.addgene.org/kits/sabatini-wood-cancer-pathway-orfs/ Finding...Technologies from 2015 Additional Resources on Addgene.org Find Other Kits for Your Research Find Pooled... Kendall Morgan...

...Communication Additional Resources on Addgene.org Find Job Openings at Addgene ...started:  Subscribe to Career Blog Posts from Addgene 1) Twitter – Get your voice out there Here’s one...today, Soapbox Science (hosted by Nature), and Addgene are open to guest contributions. If you decide ...BananarootsBlog.   Additional Resources on the Addgene Blog Check out Other Science Communication Posts...work or volunteer for a science communication organisation like Building with Biology. Local environmental...

... wide range of fields from Addgene. What you might not know is that Addgene distributes curated collections...Sciences 108.38 (2011): 15870-15875. See Kendall Morgan’s blog post on the PUF Assembly Kit for some insights...to deposit a kit, please contact us at depost@addgene.org. ...

...appreciate the humor in science and in the lab? Addgene is after all a place that encourages people to ...  Kendall Morgan...

...protein-by-protein basis. However, since many of Addgene’s SunTag plasmids have coveted “blue flames,” it...PMCID: PMC4252608. Additional Resources on the Addgene Blog Sign Up to Receive the Fluorescent Protein...obtained bright images of mitochondria without organelle toxicity. First generation (v1) SunTag is expressed...about Small Molecule Fluorphores Resources on Addgene.org Find Fluorescent Protein Empy Backbones Visit...

...sequencing, and optical pooled screens in human cells. Addgene offers vectors for CROPseq-multi using selection...recombinant antibodies were recently added to the Addgene repository of tools for SAR-CoV-2 research. Two...critical component for viral genome packaging. Addgene recommends both for use in western blots (Figure...as a loading control (right panel). Image from Addgene. These antibodies are recombinant versions ... out the new and noteworthy tools featured in Addgene’s Neurodegeneration Research Collection. This page...vitro and in vivo, including in human retinal organoids and live mice. Importantly, REVeRT avoids the ...

...vectors Learn about Addgene AAV Quality Control Process Resources on Addgene.org Find ready-to-use AAV...PubMed PMID: 25665714. Additional Resources on the Addgene Blog Check out our Viral Vectors Featured Topic... AAV in Addgene's Viral Service Check out our AAV Production Protocol Check out our AAV Purification Protocol...investigated including lentiviruses, adenovirus, and inorganic delivery tools such as nanoparticles. These technologies...

...the Mulitplex CRISPR/Cas9 Assembly System Kit at Addgene here. Other Popular CRISPR Posts: How to Design... Kendall Morgan...

...additional pooled CRISPR libraries, also available at Addgene, enabling systematic investigation of the cellular...the use of pooled libraries in your experiments, Addgene recommends that you review the steps for how to... Kendall Morgan...

...quantity and purity.  Additional resource on the Addgene blog Browse blog posts on plasmid cloning Read...the process of inserting exogenous DNA in a microorganism. The amount of plasmid DNA that is necessary... conjugation, and transfection Resources on Addgene.org Read the molecular biology reference Find plasmids...

...right place. Get started with PGC-1 plasmids from Addgene. References: Skeletal muscle PGC-1α1 modulates... Kendall Morgan...

...microbiology Resources on Addgene.org: Visit the CRISPR Guide Get CRISPR plasmids from Addgene ...Plasmids for each of these systems are available from Addgene. If you’ve given any of these systems a try, we...acssynbio.0c00073   Additional resources on the Addgene blog: Browse CRISPR articles from our CRISPR topic...on the escape mechanism of genome editing in microorganisms which could be applied to optimization of genome...

...open access journals.   Check Out Addgene Videos Over at the Addgene Youtube Channel   After watching ...Communication 2.2 (2014): 2. Additional Resources on the Addgene Blog 6 Tools & Tips: Online Social Networking ...study processes for diagnosing and resolving organizational performance and often have to be able to quickly...Sharing in Research Publications Resources at Addgene.org Check out our Protocol Pages for Video Protocols...

...standardize your experiments later. Stay tuned to the Addgene blog and be sure to check out our viral vector ... PMCID: PMC330758. Additional Resources on the Addgene Blog Learn All about Adenovirus Associated Virus...PMID: 12036457. 6. Davis, Howard E., Jeffrey R. Morgan, and Martin L. Yarmush. "Polybrene increases retrovirus...Lentiviral Vectors Additional Resources on the Addgene.org Check out Our Viral Vector Guide Pages Find ...

...details and protocols are available online on Addgene's CRISPR Library Reference page.    References... Kendall Morgan...

...in setting up a FRET-based assay or screen (the Addgene blog has an excellent primer on FRET and how to...presented (Fig. 1) which can be carried out using Addgene plasmids. His-tag labeling reagents You may already...Gradia Lab has deposited a gateway vector with Addgene, where you can insert your protein of interest,...PubMed Central PMCID: PMC4380784. Resources on the Addgene Blog Get Tips for Using FRET in Your Experiments...unaffected by intervening proteins, lipid bilayers, organelles or other cellular impediments. FRET has been ...Your Fluorescent Protein Basics Resources on Addgene.org Find Plasmids for FRET Browse Empty Plasmids...

...pre-existing toolbox for adenoviral vectors in Addgene’s repository is smaller than for other vector types...1566523213666131125095046. PMID: 24279313. More resources on the Addgene blog Viral Vectors 101: Viral Applications Adenoviral...new adenoviral vectors. This post was written by Addgenie Brian O'Neill.  References and resources References....73.8.6930-6936.1999. PMID: 10400791. Kafri, T., Morgan, D., Krahl, T., Sarvetnick, N., Sherman, L., & ...

...features of a plasmid. So, how do you choose? Addgene Senior Scientist Marcy Patrick says researchers... Kendall Morgan...

...mammalian brain (Wickersham et al., 2007; plasmids at Addgene). AAV vectors: A platform for delivering neuronal...their capsid proteins. For a brief overview see Addgene’s AAV guide. AAV particles are relatively small ... production, purification and titration on the Addgene website).  Tissue Optimal Serotype CNS AAV1...Serotypes and their target tissues (adapted from Addgene's AAV guide)  Commonly used serotypes include AAV1...learning and memory (Kohara et al., 2014; plasmids at Addgene). The Gradinaru Lab at the California Institute...the brain based on its unique hue (plasmids at Addgene). A large-scale example of neuronal tracing with...other AAV serotype (Tervo et al., 2016; plasmid at Addgene). Its efficiency is comparable to conventional ...