SunTag and Fluorescent Imaging
Type
Blog Post
...often better. More transcription factor binding sites in a promoter lead to higher transcriptional activation...This workaround risks cellular phototoxicity and eliminates the possibility of long-term imaging studies....Overexpressed proteins also have the potential to form aggregates and may be toxic to the cell.
Here comes the ...kinesin across microtubules.
Tanenbaum et al. then tested their hypothesis that the lower expression levels...