...selection to isolate plants with the phenotype of interest. Genome engineering, on the other hand, allows...tumefaciens as a vehicle to deliver your gene of interest into a target cell line or organism (Presented...for Golden Gate mediated cloning of your gRNA of interest. In recent years, deaminase-mediated base editing...

...Google.slides or Powerpoint file right now to capture interesting key results, striking images, creative ideas,... write itself when the time comes. 5. Choose interesting content Talk about something you know a lot about...commercialized, just talking about the possibility is interesting. Check out Joanne's Reddit AMA 6. Practice, practice...

..., please refer to the paper. Why might one be interested in state machine technology? Biological research...and/or sortable reporters in certain states of interest. For example, in Figure 2 below we present a two-input...researcher in the Lu Lab at MIT. He is broadly interested in biocomputing and biological circuits.     ...

...able to knock-in a 2xFLAG epitope into my gene of interest, I encountered low efficiency (0.13%) and laborious.... Figure 1: In the background are 504 restriction digests of PCR products used to identify a single...CRISPR plasmids page. Future perspectives It is interesting to note that recipients for the 2015 Breakthrough...

...million results (Here’s the top one, in case you’re interested). “But I’m an introvert!” is not an excuse. Every...someone else on that team is putting their own self-interest ahead of the team’s goals, then that team is destined...other person really is acting against the team’s interests or not; once that seed of distrust is planted,...

...chemogenetic/optogenetic constructs into our cells of interest. Although they have a small packaging capacity...chemogenetics vs. optogenetics) I’m particularly interested in using chemogenetic and optogenetic AAV-based...2001): 1248. PubMed PMID: 11509958. 8. Ferrari, Forrest K., et al. "Second-strand synthesis is a rate-limiting...

...DNA from an Agarose Gel You may also like... Restriction Digest of Plasmid DNA Agarose Gel Electrophoresis...technique for molecular cloning, such as PCR - or restriction enzyme -based cloning. Protocol: Gel Purification...lanes between samples and between the ladder and nearest sample. To minimize the risk of DNA damage, it ...

...focus fluorescence from reaching a detector or restricting fluorescence to specific excitation volumes to...reduction in signal intensity. 2-Photon microscopes restrict excitation (and bleaching) to a single focal point... a large axial range. 2-photon imaging, which restricts excitation and photobleaching to the focal plane...

...to a fluorophore. When paired with a protein of interest, these tools provide an easier means to identify...of cellular components, typically a protein of interest. Fluorescent dyes and ligands vary in their chemical...fluorescent ligands can elicit varying degrees of β-arrestin-2 recruitment to receptors, indicating their agonistic...

...depending on the sample type and the protein of interest. You may need to try a variety of fixation methods...transfected with a plasmid to express the protein of interest, and a negative control sample such as cells that... that do not express the protein of interest....

...transposase. The donor plasmid contains the gene(s) of interest flanked by terminal repeat sequences, which the...plasmids that express the reprogramming factors of interest. The advantages of this method are that it’s relatively...KLF4 LIN28 Check out these plasmids if you’re interested in Tet-inducible lentiviral expression of OSKM...

...dithiothreitol, DTT), Invitrogen NP0009 PageRuler Plus Prestained protein ladder, Invitrogen 26619 Pipette tips...the hot plate to 100 °C. Thaw IgG standard and prestained protein ladder on ice. Reagent Preparation Prepare... that the chamber is sealed. Load 5 µL of the prestained protein ladder to the appropriate well. Pro-Tip...

...2 - Getting high school students and teachers interested in JEI (the importance of word of mouth and personal...required for cytokinesis in JEI. Developing an interested in biology, working in a lab for the first time...

...Michigan State University with the backing of a prestigious Fulbright Fellowship. Dr. Kwapata now works as...genetics and biotechnology for Horticulture and Forest Crops at the Lilongwe University of Agriculture...

...move from microbiology to law? How did you get interested in pursuing law? BN: Life science was always ... general counsel at a small start-up is very interesting. It’s very varied. There’s IP work to do, general...

...and country. This information can be of personal interest to the depositor, but it can also be valuable ...annual reports. While the raw data is, of course, interesting to parse through, aggregation of this information...

...specificity allows scientists to target proteins of interest for labeling, quantification, purification, chromatin...the spleen of mice injected with the antigen of interest. The immortalized cells, called hybridomas, are...

...decision. In the open-access Data Hub, researchers interested in the Addgene antibody collection can look at... selecting an antibody! We encourage everyone interested in the Addgene antibody collection to use and...

...Even that grim estimate might prove to be an underestimate. Given all the excitement and speculation, we...ongoing outbreak from the CDC and WHO. - For anyone interested in EBOV’s past, present, and future, don’t miss...

...high-throughput next generation sequencing (NGS). Select a restriction enzyme for digest that will cut the shared plasmid...visualize that digestion on an agarose gel ( see restriction digest protocol here ). Lentiviral plasmids can...closed system of a crowded plate or culture. Try restricting growth to the bare minimum by growing at 30 ℃...