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  4. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ...Loures, L. et al. A modular cloning toolkit for genome editing in plants. BMC Plant Biol 20, 179 (2020...

  5. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ...on the Addgene Blog  Read about Other Means of Genome Engineering Using FLEx AAV Vectors? Learn about...

  9. Synthetic Biology - Strains

    Type
    Collection
    ...SynBio Assembly Standards SynBio Blog Genome Engineering Cloning & Genomic Tools Metabolism Networks & Gene...

  10. Synthetic Biology - Metabolism

    Type
    Collection
    ...SynBio Assembly Standards SynBio Blog Genome Engineering Cloning & Genomic Tools Metabolism Networks & Gene...

  12. Lentiviral Prep Service

    Type
    Collection
    ...interrogate the genome in a variety of ways. Cas9 proteins can be guided to specific genomic sites by an ...resource page . Cas9 for Editing Genomic Sequences To perform genome edits, Cas9 nuclease is used to generate...

  13. Synthetic Biology - Overview

    Type
    Collection
    ...Category Cloning and Genomic Tools Browse plasmids related to cloning and genomic modification, including...Browse All SynBio Assembly Standards SynBio Blog Genome Engineering SynBio Depositing Labs Hal Alper J....

  14. Zinc Finger Consortium Reagents

    Type
    Collection
    ... these reagents are provided below: Zinc Finger Genome Engineering Reagents Zinc Finger Arrays for Zebrafish...expressing zinc finger FokI nuclease fusions Related Genome Engineering Technologies TALEN Engineering Reagents...

  15. CRISPR Plasmids - Tagging

    Type
    Collection
    ...Protein Tagging Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...directly into this vector and targeted to the AAVS1 genomic safe harbor locus using untagged SpCas9 (Addgene...Zhang lab's PX330 , simultaneously targeting the genomic site and the donor vector. The PITCh donor plasmid...vectors to insert genetic tags in the C. elegans genome. The SapTrap reaction produces a single plasmid...-programming sgRNA and HR donors for selectable genome modification. This system can be used to create...

  16. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ...: dCas9-FokI Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...dCas9-FokI to dimerize at a given region of the genome. Like nickase technology, this technique reduces...

  17. EXPRESs Plasmids for Zebrafish Receptors

    Type
    Collection
    ...extracellular protein interactions. Bushell KM et al., Genome Res 2008 Apr;18(4):622-30. PubMed 18296487 A cell...leucine-rich repeat receptors. Sollnet C & Wright GJ. Genome Biol 2009;10(9):R99 PubMed 19765300 Construction...

  18. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...: Drosophila Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...loss of function alleles. To introduce specific genomic changes, researchers use ssDNA or dsDNA repair ...the nickase to the target site by homology to a genomic DNA locus and encodes a primer binding site and...

  19. Retrovirus Plasmids

    Type
    Collection
    ...gamma-retrovirus) is an RNA virus comprised of its genome and several structural and enzymatic proteins, ...This provirus then gets integrated into the host's genome by the accompanying integrase protein. When scientists...γ-retroviruses integrate randomly into the host's genome, which can lead to oncogenesis (termed insertional...

  20. Hot Plasmids - October 2020

    Type
    Blog Post
    ...protein identified exclusively in huge bacteriophage genomes. It’s active in vitro and in human and plant cells...
Showing: 401 - 420 of 535 results