..., please refer to the paper. Why might one be interested in state machine technology? Biological research...and/or sortable reporters in certain states of interest. For example, in Figure 2 below we present a two-input...researcher in the Lu Lab at MIT. He is broadly interested in biocomputing and biological circuits.     ...

...Diagnostic Restriction Digest Verify a plasmid using restriction enzymes Diagnostic Restriction Digest Protocol...technique for molecular cloning, such as PCR- or restriction enzyme-based cloning. Purifying DNA from an Agarose...

...million results (Here’s the top one, in case you’re interested). “But I’m an introvert!” is not an excuse. Every...someone else on that team is putting their own self-interest ahead of the team’s goals, then that team is destined...other person really is acting against the team’s interests or not; once that seed of distrust is planted,...

...chemogenetic/optogenetic constructs into our cells of interest. Although they have a small packaging capacity...chemogenetics vs. optogenetics) I’m particularly interested in using chemogenetic and optogenetic AAV-based...2001): 1248. PubMed PMID: 11509958. 8. Ferrari, Forrest K., et al. "Second-strand synthesis is a rate-limiting...

...able to knock-in a 2xFLAG epitope into my gene of interest, I encountered low efficiency (0.13%) and laborious.... Figure 1: In the background are 504 restriction digests of PCR products used to identify a single...CRISPR plasmids page. Future perspectives It is interesting to note that recipients for the 2015 Breakthrough...

...focus fluorescence from reaching a detector or restricting fluorescence to specific excitation volumes to...reduction in signal intensity. 2-Photon microscopes restrict excitation (and bleaching) to a single focal point... a large axial range. 2-photon imaging, which restricts excitation and photobleaching to the focal plane...

...to a fluorophore. When paired with a protein of interest, these tools provide an easier means to identify...of cellular components, typically a protein of interest. Fluorescent dyes and ligands vary in their chemical...fluorescent ligands can elicit varying degrees of β-arrestin-2 recruitment to receptors, indicating their agonistic...

...transposase. The donor plasmid contains the gene(s) of interest flanked by terminal repeat sequences, which the...plasmids that express the reprogramming factors of interest. The advantages of this method are that it’s relatively...KLF4 LIN28 Check out these plasmids if you’re interested in Tet-inducible lentiviral expression of OSKM...

...producing a final plasmid that expresses the shRNA of interest. C.1 Recommended Materials Material Vendor and...for plasmids that were successfully ligated by restriction enzyme digestion. However, once you have identified...use a miniprep kit to obtain DNA. 3. Conduct a restriction digest with EcoRI and NcoI: 1 μg miniprep DNA...select for cells that stably express your shRNA of interest. E.1 Recommended Materials Material Vendor and...cells that are stably expressing your shRNA of interest. F.1. Recommended Materials Material Vendor and...

...2 - Getting high school students and teachers interested in JEI (the importance of word of mouth and personal...required for cytokinesis in JEI. Developing an interested in biology, working in a lab for the first time...

...Michigan State University with the backing of a prestigious Fulbright Fellowship. Dr. Kwapata now works as...genetics and biotechnology for Horticulture and Forest Crops at the Lilongwe University of Agriculture...

...DNA from an Agarose Gel You may also like... Restriction Digest of Plasmid DNA Agarose Gel Electrophoresis...technique for molecular cloning, such as PCR - or restriction enzyme -based cloning. Protocol: Gel Purification...lanes between samples and between the ladder and nearest sample. To minimize the risk of DNA damage, it ...

...and country. This information can be of personal interest to the depositor, but it can also be valuable ...annual reports. While the raw data is, of course, interesting to parse through, aggregation of this information...

...move from microbiology to law? How did you get interested in pursuing law? BN: Life science was always ... general counsel at a small start-up is very interesting. It’s very varied. There’s IP work to do, general...

...specificity allows scientists to target proteins of interest for labeling, quantification, purification, chromatin...the spleen of mice injected with the antigen of interest. The immortalized cells, called hybridomas, are...

...decision. In the open-access Data Hub, researchers interested in the Addgene antibody collection can look at... selecting an antibody! We encourage everyone interested in the Addgene antibody collection to use and...

...Even that grim estimate might prove to be an underestimate. Given all the excitement and speculation, we...ongoing outbreak from the CDC and WHO. - For anyone interested in EBOV’s past, present, and future, don’t miss...

...commercial kits you will adopt for the extraction. Restriction enzyme based approaches can require several hundreds...cloning grade DNA for direct cloning of your gene of interest into a plasmid backbone. Transfection grade...

...Citing Article" - such as cloning their gene of interest into a previously deposited vector - then you'...locate other papers that have used your plasmid of interest, which may help you identify relevant protocols...

...trainees at all. For grad students and postdocs interested in taking matters into their own hands, there...I am happy to work with any group of trainees interested in designing career exploration programs or peer...