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  1. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    ... on the Leith river. Photo courtesy of Leonardo Ramos.  I enrolled into a summer school course at ...confluence of science and the world it hopes to change. Most researchers are aware of the importance of science...

  2. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    ...Breakdown of Addgene's top blog posts from 2015 Our most popular posts continue to be those from our Plasmids...Download the Plasmids 101 3rd Ed. eBook Read the Most Recent Plasmids 101, CRISPR 101, and Career Posts...

  6. Plasmids 101: Origin of Replication

    Type
    Blog Post
    ... from the those used to replicate the host's chromosomal DNA, but they still rely on the host machinery...that create a toxic product. The E. coli strain Most E. coli strains can be used to propagate plasmids...

  8. Another Pathway into Cells: iTOP

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    Blog Post
    ...introduce a gene of interest into primary cells, so most primary cell lines require viral infection. A new...a new idea, but it comes with a few caveats. The most common protein-only transduction strategy is to ...

  9. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ...Postdoctoral Research Fellow with Pamela Silver.  Most types of biological motion (whether endocytosis,...cartoony explanation of R-bodies, check out this video. Most types of biological motion (whether endocytosis,...

  10. How to Lead a Great Meeting

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    Blog Post
    ...meeting with no idea why you are there. Make the most of everyone’s time by considering the level of meeting...to meet monthly, but we found our meetings took almost two hours and we felt out of touch. By moving to...

  11. Components of CRISPR/Cas9

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    Blog Post
    ...Updated Mar 26, 2020. At their most basic level, CRISPR/Cas9 genome editing systems use a non-specific...crRNAs from pre-crRNAs derived from CRISPR arrays. In most systems used for CRISPR-mediated genome editing,...

  13. "Build Your Plasmid": The Game - Play to Teach

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    Blog Post
    ...had to think creatively so as not to develop the most obvious strategy which  also could have been proposed... In the end, the students had fun while applying most of the concepts required by the course and some ...

  15. 25 Work From Home Tips for Scientists

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    Blog Post
    ...bench scientist who began working remotely recently? Most of us at Addgene began working from home last week... negative data or publish brief, novel findings. Most Micropublications contain one figure. If you aren...

  16. What Good Citizenship Can Do for Reproducibility in Science

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    Blog Post
    ...Sweet, Vice President of Editorial at Cell Press. Almost everyone who works in a lab struggles with reproducibility...research environment. Journals as enforcers Currently, most of the burden for pushing for more transparency ...

  17. Celebrate National Book Month with Addgene

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    Blog Post
    ...s writing will capture the attention of even the most skeptical reader of historical fiction (I can personally...’s The True Deceiver. There have been books that most of the group really disliked, some that everyone...

  18. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    ...Yamanaka himself, during his keynote address, appeared almost incredulous that the field has exploded so quickly...applications with the potential to save lives. While most scientists recognize and appreciate the importance...

  19. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ...pathways with sophisticated gene expression controls. Most cell types do not respond to low-intensity light...number of plasmids, with one RR (ompR) encoded chromosomally. Leakiness was also an issue; some promoter ...

  20. Choosing Your Fluorescent Proteins for Multi-Color Imaging

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    Blog Post
    ...fluorescent proteins in common use (particularly not the most recently published ones), they can be a good starting...proteins the green and red proteins are generally the most detectable and so should be used to tag your least...
Showing: 401 - 420 of 759 results