...CRISPR History and Development Resources on the Addgene blog CRISPR Antimicrobials Treating Muscular ...functional correction of the gene in clonally expanded organoids. This work, and many others, pushed the field ... post was originally written in 2014 by Kendall Morgan and updated in 2023 by Susanna Stroik.  References...of CFTR by CRISPR/Cas9 in Intestinal Stem Cell Organoids of Cystic Fibrosis Patients." Cell Stem Cell. ...1563. doi: 10.3390/ijms24021563 Resources on Addgene.org CRISPR Plasmids and Resources CRISPR 101 eBook...

...Science Guides Antibody Guide Antibody Guide View Addgene's Antibody Collection Jump To: Antibody Guide Production...applications, visit the Antibody section of the Addgene Protocols page. Antibody Applications - Quantification...intensive. Ready to put your new knowledge to use? Addgene now makes ready-to-use recombinant antibodies made...myeloma cells (immortalized B cells). The genetic reorganization that creates variety in the antibody allowing...the variable region goes through a randomized reorganization process, creating a unique binding site. This...

...we will not delete @addgene on X. This will additionally ensure that the @addgene handle is always associated...media, you can find us on Bluesky: @addgene.bsky.social; or @addgene on Instagram, TikTok, Facebook, and...an official Addgene account. You can reach out to us directly via email at help@addgene.org, and we invite...Addgene is announcing we have chosen to stop all activity on X, formerly Twitter. If you are interested...mainstay of conversation in the scientific community. Addgene has been considering a gradual move off X as a ...to paid content regardless of user preference. Addgene is a values-driven nonprofit. Recent remarks and...posting, monitoring, and resharing content on X.  Addgene deeply values our community and recognizes the ...

...PubMed PMID:18647110. Additional Resources on the Addgene Blog Read More about Fluorescent Protein Imaging...are better for whole cell imaging and studying organelle trafficking. Monomeric PA-FPs are usually preferred...involve enhancing optical live imaging of proteins, organelles, and cells. Similar to FPs, PA-FPs allow for ...localization, turnover, and trafficking, as well as organelle trafficking and dynamics (fission, fusion, etc...mitochondrial dynamics by photolabeling of individual organelles shows that mitochondrial fusion is blocked during...Catch Up on Your GFP Background Resources at Addgene.org Find Photoactivatable FPs in Empty Backbones...

...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.      Additional... Today on the Addgene Podcast, we have another interview conducted by European Outreach Scientist Benoit...Additional Resources from the Addgene Blog Visit our Fluorescent Proteins featured topic page Read Joachim's... addgene podcast...Visualizing Subcellular Structures and Organelles Resources at Addgene.org Find Fluorescent Protein Plasmids...

...cover it. Addgene: OK, thanks for your time, and thanks for depositing your plasmids with Addgene. Howarth... and its potential uses. Developing a new tool Addgene: Where did you get the idea to develop SpyLigase...just join one peptide tag to another peptide tag. Addgene: What is the advantage of peptide tags for building...bonds, so there were no weak links in the chain.  Addgene: What about the Streptococcus pyogenes protein ...pili that they use to anchor on to host cells. Addgene: Were there any major hurdles along the way? Howarth...capture system.) Cancer and other applications Addgene: What does this technology mean in the context ...capture or for capture using microfluidic devices. Addgene: What other uses do you foresee for this tool? ...

...Developing the Addgene microbiology community #ASMMicrobe2016 was a great opportunity for Addgene to let microbiologists...materials! Resources on the Addgene Blog Learn How You Can Use Addgene's Microbiology Pages Read Our ...The start of the conference After setting up the Addgene booth on Thursday evening, I stayed to see the ...with lots of researchers who weren’t aware of Addgene’s plasmid sharing services, as well as #happydepositors...dedicated to science communication through art. Addgene Advisory Board member Siouxsie Wiles gave us a ... about amazing work already represented in the Addgene repository (like these new Rainbow Bacterial Fluorescence...creating well-optimized CRISPR screening technology. Addgene got several shout-outs for making popular screening...

...two-color pair. We'll be sending it to Addgene in a matter of days. Addgene: Can you tell us a little more about..., or other conditions. Addgene: You've contributed various plasmids to Addgene over the years, many of...available. Visit Ed Boyden lab's Addgene page and click the link in the Addgene Alerts box. References: ...activity of two populations of neurons at once. Addgene spoke to Boyden about this latest optogenetics ...missing. Optogenetic tools for neuroengineering Addgene: For those who may not be experts, can you give...Understanding the brain through optogenetics research Addgene: What are some of the big problems that optogenetic...these tools, so we can focus on the next round. Addgene: What tools are still missing from the optogenetics...

...with detailed protocols, go to www.addgene.org/crispr/tagging. Addgene: What motivated you to adapt CRISPR...tagging system, which they’ve now made available at Addgene. For more details on the plasmids that are currently...can now look at many more transcription factors. Addgene: How does the system work? Mendenhall: The system...antibodies. The results look almost identical. Addgene: What advantages does your system have in this ...faster. It’s also easy to run things in parallel. Addgene: Besides being fast and efficient, what other challenges... hundred different versions of this out there. Addgene: Why was it important to you to make these plasmids...waiting and hands on labor. That’s a huge deal. With Addgene, now these can be available to anyone for a modest...

...communication blog posts Resources on Addgene.org Find careers at Addgene ... project I’d seen in my already long tenure at Addgene. I would work with our research team and software...pinpointed and (mostly) solved two main problems with Addgene’s cross team communication. Although these real ...these plates now” to a fellow team member. But, at Addgene, we use seven different types of “plates” (Fig....not the container was barcoded (this is pretty Addgene specific) The types or purposes of each given container...that there were a lot of words that nearly all Addgene teams used that seemed simple, but they could in...solution for this problem was the creation of an Addgene lexicon. The base lexicon was and continues to ...

...researchers as we could, and Addgene is a wonderful vehicle for us to do that.” At Addgene, we are equally excited...is available in an easily searchable format on Addgene’s Michael Davidson Fluorescent Protein Collection...collection with over 300 backbones alone,” said Addgene’s Lianna Swanson, who has been working with members...the scientific community," says Joanne Kamens, Addgene's Executive Director, "and to promote the great ... Kendall Morgan...

...things are easy with CRISPR. Addgene: You’ve made many deposits to Addgene over the years. Can you describe...biology, the tools he’s making available through Addgene, and where all of it is likely to lead us in the...the future. Building circuits from CRISPRs Addgene: What can CRISPR do for synthetic biology? Voigt...conceivably be as large as natural regulatory networks. Addgene: What kinds of things could we do as genetic circuits.... It requires more complex synthetic networks. Addgene: What hurdles did you have to overcome and what...regarding the size of the networks you could build. Addgene: Is this what you are working on now? Voigt: We...taking on very large genetic engineering projects. Addgene: How easy is it to do this now? Voigt: One of the...

...versions of 15 different disease-associated genes. Addgene spoke to Musunuru about how the new kit works, ...and how it compares to his CRISPR/Cas9 system. Addgene: For people unfamiliar with this new TALEN kit,...either knock out a gene or knock in a mutation. Addgene: How do you use this kit to target a particular...cells. (See the complete protocol at StemBook). Addgene: How are you now using this tool? Musunuru: We ...one needs to do disease modeling in stem cells. Addgene: Tell me about the history. How had investigators...genetically modified cells in less than a month's time. Addgene: What are the implications of this kit in terms...becomes more of an apples-to-oranges comparison. Addgene: Can you compare the TALEN kit to CRISPR/Cas9? ...

...PMCID: PMC4831023. Additional Resources on the Addgene Blog Learn More About Fluorescent Protein Aggregation...there were a variety of ways to monitor cell, organelle, and protein localization. For instance, you might...microscope, fractionate samples to isolate particular organelles and their contents, or perform in situ hybridization...Choosing Your Fluorescent Protein Resources at Addgene.org Find Fluorescent Protein Timers Browse All Fluorescent...

...way to visualize them. You can also “hear” them. Addgene depositor Dr. Mikhail Shapiro from Caltech presented...PubMed Central PMCID: PMC5920530.  Resources on the Addgene Blog Read Our Post on CRISPR Methods for Bacterial...bacteria Deposit Your Microbiology Plasmids with Addgene ...Acoustic reporter genes for noninvasive imaging of microorganisms in mammalian hosts." Nature 553.7686 (2018)...Science Communication using Twitter  Resources on Addgene.org Check out Our Microbiology Resource Page CRISPR...

...those rare diseases will receive plasmids from Addgene worth a total of $5000.  Lowe Syndrome One of those...the award from the Rare Genomics Institute and Addgene will make a substantial difference in pushing the...iPS) technology and CRISPR-Cas9 constructs from Addgene to gene-edit mutations into XP genes and other ...analyses.  “We believe that with this award from Addgene, we’ll be able to make big progress in elucidating... Kendall Morgan...

...about the ClonTracr Library Resources on Addgene.org Addgene's most popular lentiviral plasmids Overview...lentiviruses can infect dividing and non-dividing cells.  Addgene has an extensive collection of lentiviral plasmids...lentiviral questions. This useful resource from Addgene Depositor (and lentiviral expert) Didier Trono'...gritty and answer the questions those of us at Addgene get asked most often: Q1: Are retroviruses and ...and 3rd-generation transfer vectors apart? A3: Addgene defines 2nd- or 3rd-generation transfer vectors... competent? What is SIN? A6: Most (if not all) Addgene transfer plasmids are replication-deficient meaning...Trono Lab: Lentivectors Toolbox Resources on the Addgene Blog: Download our eBook: Plasmids 101 Learn about...

... al., 2019). Find the mAID tagging toolkit at Addgene Yesbolatova et al., Methods, 2019. PubMed PMID:...difficult. Find SpCas9 and Target-AID-NG plasmids at Addgene! Nishimasu H et al., Science, 2018. PubMed PMID...research and innovation. Find MerMAIDs plasmids at Addgene Opperman J et al., Nature Communications, 2019....plasmids at Addgene Kim et al., Nat Methods. 2019, PubMed PMID: 31235883. ...new family of seven ChRs derived from marine microorganisms based on metagenomic data. MerMAIDs (Metagenomically... Various Addgenies...

... for the generation of human disease models." Addgene Outreach Scientist and fellow blogger Caroline ...CRISPR Plasmids? CRISPR-Cas9 Plasmids available at Addgene CRISPR-Cas9 References, Protocols, and more References... Kendall Morgan...

...article can be obtained through Addgene: Rapamycin-inducible Cas9 sets (Addgene plasmids 62883 &62884; 62885...At Addgene we're continually impressed with the amazing plasmid technologies developed by our community... Rapamycin-inducible dCas9-VP64 activator set (Addgene plasmids 62887 & 62888) Zetsche et al., Nat ...brilliant SunTag system plasmids are available through Addgene and are already very popular. Use these plasmids...pathways (Figure 2). They are all available now at Addgene. 1 Lungu et al., Chem Biol. 2012 Apr 20;19(4..., and other plasmids from this article through Addgene. Nihongaki et al., Chemistry and Biology. 2015...human genes. The plasmids are all available at Addgene ready to light up the genome of your favorite cells...