...transferred from B. subtilis to the bacterium of interest following IPTG induction of conjugation genes ... transferred to a recipient bacteria strain of interest by tri-parental mating. Donor bacteria contain...ready to move Mobile-CRISPRi into your bacteria of interest? Find Mobile-CRISPRi plasmids at Addgene! References...

...tools allowed us to detect and capture proteins of interest to better study them in vivo and in vitro, especially... was to recombinantly append their proteins of interest with unrelated peptides that already had high ...only be added to the C-terminus of proteins of interest and proteins tagged with it needed to be chemically...

...with your transfer plasmid, carrying your gene of interest, and the two helper plasmids, commonly called ...Addgene AAV Titration by qPCR Protocol. If you’re interested in the ddPCR titering process, which is what ...non-genome-containing (empty) capsids, thus leading to an overestimate of transducible units in the virus preparation...

...pathway that are of interest to their laboratory. A single mouse click on a gene of interest will direct the...Michael G. Lemieux is a Scientist at Addgene with interests in education and scientific writing.     References...

...target near the last coding exon of your gene of interest. For ideal use, this gRNA should be cloned into...endogenous promoter and the 3’ UTR of the locus of interest, since the GFP-Puro will be inserted just upstream...nucleases and their applications. It will be interesting to see what new editing possibilities MMEJ can...

...gradients use dilutions of your sample or protein of interest to understand the dynamic or working range of ...particularly in the sizes around your protein of interest, but you don’t want to run so long your dye front...membrane. If you’re not sure if your protein of interest is over-transferring (going completely through... validated for your protein and application of interest or validating an antibody yourself. But even with...

...have children but are interested in testing your SciComm skills or are interested in a career in education...Too complicated and I would quickly lose their interest. I won’t claim that I blew their minds, but I ...

...outcomes on viability if cells are arrested too long. Prolonged cell cycle arrest can lead to apoptosis in as... you don’t accidentally change your protein of interest.   Fig. 2: Stop re-cutting with PAM and...

... and efficient tools to introduce your gene of interest into cells. Unlike gamma-retroviruses that can...vector, a transfer vector including the gene of interest, and the plasmid coding for an envelope. Three...LTR promoter to drive expression of the gene of interest. Consequently, the levels of protein expression...

...producing a final plasmid that expresses the shRNA of interest. C.1 Recommended Materials Material Vendor and...for plasmids that were successfully ligated by restriction enzyme digestion. However, once you have identified...use a miniprep kit to obtain DNA. 3. Conduct a restriction digest with EcoRI and NcoI: 1 μg miniprep DNA...select for cells that stably express your shRNA of interest. E.1 Recommended Materials Material Vendor and...cells that are stably expressing your shRNA of interest. F.1. Recommended Materials Material Vendor and...

... this mutation by an adenine base editor would restore antibiotic resistance. To find such a protein, ...editing causes the creation or destruction of any restriction enzyme (RE) recognition site, RE analysis of ...University, University Park, USA. Dr. Molla is interested in precise genome editing in rice and uses base...

...probability. Continuous inversion of the DNA sequence of interest can lead to poor expression of genes within that...lines that express Cre only in the cell type of interest (Atasoy et al. 2008; Cardin et al. 2009). Ligand...plasmids and the low concentration of recombinase restricts their expression to individual cells. With this...

...DNA or RNA nucleotides containing a sequence of interest (red) is amplified using Recombinase Polymerase...in complex with crRNA targeting the sequence of interest and an inactivated fluorescent RNA reporter. If...specifically targeted RNAs. Finally (and perhaps most interesting), Cas13a’s propensity to cleave any RNAs after...

...discover new ways to reach our colleagues and other interested audiences quickly and concisely – video might...share any details about their research itself. Pinterest is also a favorite for millions and their visual...decide if reading more about the research is of interest. In his research, Scott Spicer concludes that ...

...isn’t for everyone, particularly if you’re more interested in selling a product than communicating your ...Reddit has a large and active community of people interested in science. You can see some of the conversations...prepared! The mechanics of doing the AMA were interesting. I started watching the questions and preparing...

...statistical methods, omission of some data, etc.  The firestorm that followed implementation of the policy comprised...Lastly, data is our raw product. If we don’t take interest in how it is released, we will be in danger of... publishers and funders. Clearly, it is in our interest to ensure that our data are reproducible and useful...

...necessary for getting the plasmid express our gene of interest. This element is responsible for initiating the...role in determining where and when your gene of interest will be expressed.      The RNA polymerase...results or even kill your cells if your gene of interest is toxic. To combat this, scientists have created...

...that’s what keeps things interesting. And hopefully they’ll be just as interesting as the finished product...partially reopened. But there were still many restrictions in place, most importantly, masks.  Besides ...

...together as an interest group (15) While marchers are a group with one unifying interest (the advancement...thought of as just another party. Additionally, interest groups are often pandered to when strong or overlooked...

...selection to isolate plants with the phenotype of interest. Genome engineering, on the other hand, allows...tumefaciens as a vehicle to deliver your gene of interest into a target cell line or organism (Presented...for Golden Gate mediated cloning of your gRNA of interest. In recent years, deaminase-mediated base editing...