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  1. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...Broad GPP genome-wide Brunello 73179 (1 plasmid) 73178 (2 plasmid) Knockout Human Doench and Root 3rd 4 ...76,441 Broad GPP genome-wide Brie 73632 (1 plasmid) 73633 (2 plasmid) Knockout Mouse Doench and Root 3rd 4 ...GPP kinome Brunello 75314, 75315 (1 plasmid) 75312, 75313 (2 plasmid) Knockout Human Doench and Root 3rd... 3,052 Broad GPP kinome Brie 75317 (1 plasmid) 75316 (2 plasmid) Knockout Mouse Doench and Root 3rd 4 ...20,051 Human GeCKO v2 1000000048 (1 plasmid) 1000000049 (2 plasmid) Knockout Human Zhang 3rd 6 123,411...185,836 Mouse GeCKO v2 1000000052 (1 plasmid) 1000000053 (2 plasmid) Knockout Mouse Zhang 3rd 6 130,209... - 3 plasmid system 1000000075 (Puromycin) Activation Mouse Zhang 3rd 3 69,716 SAM v2 - 2 plasmid system...

  2. Nuclear Receptor Signaling Atlas (NURSA) Plasmid Guide: Nuclear Receptors

    Type
    Collection
    ... Plasmid Collections Nuclear Receptor Plasmid Collection Nuclear Receptor Plasmid Collection...Receptor Plasmids Find Plasmids by Gene Name Alternative Gene Names Gene Description AR plasmids AR, RP11... Nuclear Receptor plasmid collection for the community. If you have created plasmids that you would like...ESRRB Plasmids ESRRB, DFNB35, ERR2, ERRb, ESRL2, NR3B2 estrogen-related receptor beta ESRRG Plasmids ESRRG...group A, member 1 PGR Plasmids PGR, NR3C3, PR progesterone receptor PPARA Plasmids PPARA, SC22CB-5E3.5,...receptor gamma RARA Plasmids RARA, NR1B1, RAR retinoic acid receptor, alpha RORA Plasmids RORA, ROR1, ROR2...orphan receptor C RXRA Plasmids RXRA, NR2B1 retinoid X receptor, alpha RXRG Plasmids RXRG, NR2B3, RXRC retinoid...

  3. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Neurodegeneration Neurodegeneration Plasmids Neurodegeneration Plasmid Collection Addgene has a large collection...Browse all Neurodegeneration Plasmids Do you have suggestions for other plasmids that should be added to this...Browse neurodegeneration plasmids for research related to Alzheimer's Disease, ALS, Frontotemporal Dementia...collection of neurodegeneration plasmids for research related to Alzheimer's Disease (AD), Amyotrophic...others. The table below can be sorted by disease, plasmid type, mutations, and/or tags such as fluorescent...particular gene of interest, please search our full plasmid collection using the search bar at the top of this...Tags GFP FP other Flag GST His TEV Other, non-FP Plasmid Type Mammalian, non-viral Lentiviral AAV Retroviral...

  4. Qi Lab CRISPR Page

    Type
    Collection
    ... CRISPR Qi Lab CRISPR Plasmids Qi Labs CRISPR Plasmids Available from... genome. 1. Two-plasmid CRISPRi system for bacterial gene knockdown The first plasmid ( pdCas9_bacteria... both plasmids in bacteria could cause up to 300-fold repress on targeted genes. 2. Two-plasmid CRISPRi...fusion expression plasmid 44248 pgRNA-humanized A customizable gRNA expression plasmid for use with either...bacterial Cas9 expression plasmid 44250 pwtCas9-bacteria A wild-type Cas9 expression plasmid for use editing bacterial...2013 Jul 9. doi: 10.1016/j.cell.2013.06.044. PubMed PMID 23849981 . Individual plasmids can be ordered ...CRISPRi Cas9 gRNA plasmids from the Qi lab CRISPR...

  5. Biosensor AAV Preps

    Type
    Collection
    ...retrograde serotype, produced with the AAV retro helper plasmid from the Karpova and Schaffer labs. See the Retrograde...options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need....Looger 101060 pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40 Syn CaMPARI2 his Constitutive 1 Schreiter 101061 pAAV_hsyn_NES-his-CaMPARI2...Syn CaMPARI2-F391W his Constitutive 1 Schreiter 101064 pAAV_hsyn_NES-his-CaMPARI2-L398T-WPRE-SV40 Syn ...

  6. mTOR Pathway

    Type
    Collection
    ...proliferation. mTOR Pathway Plasmids Click on a name to find available plasmids for the gene, or browse the...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Addgene's plasmids for the mTOR pathway. enhanced signaling of mTOR, a key metabolic regulator, correlates...with a New Screening Kit Background mTOR Pathway Plasmids mTOR Gene List Resources The m echanistic or m... - Gene List Click on a name to find available plasmids for the gene. For components with multiple isoforms...Sabatini DM. Cell. 2012 Apr 13;149(2):274-93. doi: 10.1016/j.cell.2012.03.017. PubMed PMID: 22500797 . Rapamycin...Blenis J. Cell Metab. 2014 Mar 4;19(3):373-9. doi: 10.1016/j.cmet.2014.01.001. PubMed PMID: 24508508 . Targeting...

  8. Zhang Lab's CRISPR Frequently Asked Questions

    Type
    Collection
    ... may also like... Zhang Lab CRISPR plasmids General CRISPR Plasmids Dr. Le Cong, the first author of one...easiest way is to co-transfect several plasmid together, with each plasmid having a targeting spacer cloned ... Genome Engineering CRISPR Zhang Lab CRISPR Plasmids Zhang Lab CRISPR FAQs Zhang Lab's CRISPR Frequently...with expression, though it is possible for the plasmid to still express without the G. Because the G is...experiments for multiplexed targeting with Zhang lab plasmids? For multiplexing CRISPR to target multiple genome...into PX330, then co-transfect or delivery the two plasmids together into your cells. If you want to use double...very big concern, you could go ahead with PX330 plasmid, clone in your target guides, test run them and...

  9. Zhang Lab CRISPR Page

    Type
    Collection
    ...tracrRNA: This plasmid contains three expression cassettes. To target a given site, the plasmid can be digested...adenocarcinoma pathology. These plasmids as well as a backbone plasmid for cloning new targets are described...sgRNA backbone for cloning new targeted plasmids. The plasmid can be digested using SapI, which will reveal...2015 Apr 1. PubMed . Return to AAV In vivo plasmids AAV Plasmids with Cas9 Mouse CRISPR-Cas9 knockin mice.... doi: 10.1016/j.cell.2014.09.014. Epub 2014 Sep 25. PubMed . Return to AAV Cas9 mouse plasmids...

  10. The Pleiades Promoter Project

    Type
    Collection
    ... Plasmid Collections Pleiades Promoter Plasmids Pleiades Promoter Project The...using the links to view individual plasmid webpages. These plasmids are described in Portales-Casamar ...Plasmids from the Pleiades Promoter Project - A regulatory toolbox of MiniPromoters to drive selective...human MiniPromoters. Browse the Pleiades Promoter Plasmids using the table below. Additional information ... pEMS1371 EGFP/NLS Ple100 GRP pEMS1372 EGFP/NLS Ple101 GRP pEMS1373 EGFP/NLS Ple102 GRP pEMS1374 EGFP/...Ple232 TNNT1 pEMS1704 intron-lacZ/NLS Ple233 TNNT1 pEMS1101 EGFP/cre/NLS Ple233 TNNT1 pEMS1437 EGFP/NLS Ple234...

  11. Genetic Code Expansion

    Type
    Collection
    ...Generally, you will need two plasmids, as depicted in the figure below: A plasmid expressing the tRNA and ... included. Browse Synthetase Plasmids The table below highlights plasmids that contain aminoacyl tRNA ...Many of the plasmids also contain one or more copies of the cognate tRNA gene. ID Plasmid Synthetase Origin...Abhishek Chatterjee Browse Target Plasmids The table below highlights plasmids that contain genes with modified...Addgene's guide to plasmids and strains useful for expanding the genetic code in bacteria and mammalian... Plasmid Collections Genetic Code Expansion Genetic Code Expansion You may also like... GCE4All...tRNA synthetases are introduced into the cell on plasmids and the new amino acid is introduced in the media...

  12. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ... on the plasmid. gRNA plasmids that do not co-express a Cas protein require a separate plasmid that does...gRNA Vectors CRISPR Plasmids: Empty gRNA Expression Vectors Browse CRISPR Plasmids By Function Genome ... these independent gRNA plasmids can be paired with a wide variety of plasmids and therefore are not limited.... Whether the plasmid contains Cas9 and if so, which function of Cas9. We have plasmids with Cas9 that...none, need plasmid 65775 N. meningitidis Joung VVT1 65779 Mammalian BsmBI none, need plasmid 65776 S. aureus...expression of Csy4 (eg Plasmid 53369 ). Joung pX333 Mammalian Single all-in-one plasmid for tandem expression...types of plasmids available for use in this Golden Gate and Gateway based assembly method: plasmids for assembly...

  13. Validated gRNA Sequences

    Type
    Collection
    ...CRISPR experiments. This datatable is compiled from plasmids in Addgene's database as well as from sequences..., the categories described on Addgene's CRISPR Plasmids and Resources page have been used to indicate ...Datatable Target Gene Target Species Target Sequence Plasmid ID Application Cas9 Species PubMed ID Depositor...ACCATCTAATTCAACAAGAATT 73221 interfere S. pyogenes 26689101 Hudson pgi C. glutamicum TGACCGATCATTACTCAAACTTCC...ACCATCTAATTCAACAAGAATT 73224 interfere S. pyogenes 26689101 Hudson xylR C. beijerinckii CGAGTTAGACATAATAGTGA...

  14. CRISPR Guide

    Type
    Guide
    ...editing CRISPR 101: Homology directed repair CRISPR 101: Validating your genomic edit CRISPR 101: Multiplex...Blog References Website CRISPR plasmids and resources at Addgene CRISPR 101 eBook (3rd edition) CRISPR software...Addgene's guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening. Science... cell must be validated experimentally. Browse Plasmids: Double-Strand Break (Cut) Multiplex Genome Engineering...cases, delivering multiple gRNAs using a single plasmid ensures that all gRNAs will be expressed in the...likelihood that any cell containing the CRISPR plasmid will have all desired genomic edits carried out...genetic loci by cloning multiple gRNAs into a single plasmid — though some have achieved targeting in the double...

  15. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ... Biosensors Luciferase Plasmids Subcellular Localization Optogenetics Plasmids Viral Service: AAV Biosensors...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Collection of plasmids encoding fluorescent biosensors to measure biomolecules or genes via FRET or other... Plasmid Collections Fluorescent Proteins Fluorescent Biosensors Fluorescent Proteins: Biosensors...our catalog . Use the article links to find the plasmids associated with the article. We also offer ready-to-use...ready-to-use AAV preparations of select plasmids; these are noted with the viral service icon . Metal Ions... localized dTomato fluorophore Ting Lab GCaMP6 Plasmids 2014 Jonathan Ting Calcium Intersectional viral...

  16. EXPRESs plasmids for Plasmodium vivax

    Type
    Collection
    ...vivax EXPRESs Plasmids for Plasmodium vivax You may also like... Julian Rayner Lab plasmids Gavin Wright...Wright Lab plasmids This is a collection of plasmids that was created for systematic screening of extracellular...stage of the malaria parasite, P. vivax . Plasmid ID Plasmid Name 68505 MSP1-bio 68506 MSP3.1-bio 68507...merozoite protein plasmids for screening protein interactions Special... Special Collections EXPRESs Plasmids Plasmodium...68535 PVX_116775-bio 68536 RBP2-like-bio 68537 PVX_001015-bio 68538 PVX_110945-bio 68539 PVX_110950-bio...

  17. Structural Genomics Consortium Plasmids

    Type
    Collection
    ... Plasmid Collections Structural Genomics Consortium Plasmids Structural Genomics Consortium... Consortium Plasmids You may also like... David Waugh Lab plasmids The Structural Genomics Consortium ...available to request. Browse the SGC plasmid collection by All Plasmids SGC Toronto SGC Oxford SGC Stockholm...Stockholm Bacterial Expression Plasmids (empty backbones/controls): ID Plasmid GenBank Key Features 26092 ...Request Plasmids To order, Login or Register a new account. Add the kit or individual plasmids to your...Plasmids from the Structural Genomics Consortium (SCG), which are primarily used for determining protein...the public domain without restriction. Many SGC plasmids from the Toronto, Oxford and Stockholm sites have...

  18. EXPRESs plasmids for human erythrocytes

    Type
    Collection
    ... Special Collections EXPRESs Plasmids Human Platelet EXPRESs Plasmids for Human Erythrocyte Receptors...may also like... Gavin Wright Lab plasmids This is a collection plasmids for expressing secreted recombinant...2011 Nov 9;480(7378):534-7. PubMed 22080952 Plasmid ID Plasmid Name 73089 bio-CD4d3+4-Kell blood group 73090...-CD4d3+4-bio 73100 Basigin-isoform1-CD4d3+4-bio 73101 Lutheran-CD4d3+4-bio 73102 ICAM4-CD4d3+4-bio 73103...

  19. FlyCRISPR

    Type
    Collection
    ... CRISPR flyCRISPR Lab CRISPR Plasmids flyCRISPR Plasmids Available from Addgene You may also like...window) Article Individual plasmids can be ordered via the links below: ID Plasmid 46294 pBS-Hsp70-Cas9 : ...Gratz, et al. (2013). Plasmid is low copy number. 45946 pU6-BbsI-chiRNA : Plasmid for expression of chiRNA...Browse CRISPR Cas9 gRNA plasmids for drosophila research from the O'Connor-Giles, Wildonger, and Harrison...within a month. phsp70-Cas9 and a single chiRNA plasmid can be coinjected to generate mutations via imperfectly... repaired DSBs. Coinjection of phsp70-Cas9 and plasmids encoding two chiRNAs can induce defined deletions...cloned by annealed oligos into the pU6-BbsI-chiRNA plasmid via the BbsI restriction sites. Design oligos based...

  20. EXPRESs Plasmids for Plasmodium falciparum

    Type
    Collection
    ...EXPRESs Plasmids Plasmodium falciparum EXPRESs Plasmids for Plasmodium falciparum You may also like........ Gavin Wright Lab plasmids This is a collection of plasmids that was created for systematic screening...2011 Nov 9;480(7378):534-7. PubMed 22080952 Plasmid ID Plasmid Name 36145 ama1CD4d3+4-bio 36146 ama1CD4d3...list of EXPRESs plasmids used with AVEXIS for screening in Plasmodium falciparum. Plasmid... Plasmid Collections EXPRESs..._1136200-bio 47731 MSP3-bio 47732 MSP6-bio 47733 H101-bio 47734 MSP11-bio 47735 MSP7-bio 47736 MSRP1-bio...
Showing: 381 - 400 of 408 results