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  5. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    ...Protein-DNA Interactions CRISPRainbow and Genome Visualization CRISPR Between the Genes: How to Experiment...methods and tools for studying chromatin and the epigenome. If you have been considering some new techniques...Regulation CRISPR 101: Epigenetics and Editing the Epigenome CUT&RUN: An Improved Method for Studying Protein-DNA...Experiment with Enhancers and Epigenomics I would like to thank the many scientists with me at the Keystone ...

  11. Clear and Accessible Writing

    Type
    Blog Post
    ...are captured at the 17%, 25%, and 60% layers, and genome-containing virions are captured at the 40% layer...

  13. Working with Nuclear Receptors

    Type
    Blog Post
    ...receptors and signaling cascades conceivably allows the genome to sense the impact of the total environment on...

  14. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...loss of function alleles. To introduce specific genomic changes, researchers use ssDNA or dsDNA repair ... Publication Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...the nickase to the target site by homology to a genomic DNA locus and encodes a primer binding site and...Cas9 (dCas9) can be used to purify a region of genomic DNA and its associated proteins, RNA, and DNA. ...pLtetO-1 Fujii Efficient isolation of specific genomic regions and identification of associated proteins...

  15. CRISPR Plasmids - Parasites

    Type
    Collection
    ...Plasmids: Parasites Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...loss of function alleles. To introduce specific genomic changes, researchers use ssDNA or dsDNA repair ...Knockout Pooled Library - Toxoplasma gondii CRISPR genome-wide knockout gRNA pooled library designed with...

  16. CRISPR Plasmids - CRISPR Transposases (CAST)

    Type
    Collection
    ...Transposases (CASTs) Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...multiple components) to target locations in the genome defined by a guide RNA. The transposase can then...insertions. CASTs are most often used in bacteria genome editing, though some mammalian systems have been...

  17. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ... plasmids for CRISPR-Cas and Cascade for large genomic deletions....Cascade-Cas3 Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...into a DNA “shredder” system that can cause large genomic deletions. Compared to deletions generated by Cas9...

  18. Bacterial Expression Systems

    Type
    Collection
    ... and mutations to the bacterial genome itself. Manipulating the genome directly can give you more subtle...Expression Systems Bacterial Expression Systems Genome Engineering Visualization and Tagging Purification... bacterial expression systems to find out more! Genome Engineering Although overexpressing or knocking...there are many different ways to alter bacterial genomes. From recombineering to CRISPR , this collection...variety of tools to help you harness bacterial genomes for your research. Plasmid ID Technique PI Purpose... attachment site found in many enterobacterial genomes. The bacteria of interest are transformed with ...vectors for protein purification from the Structural Genomics Consortium and Nicola Burgess Brown containing...
Showing: 381 - 400 of 535 results