... comes in. Let’s take a look at All_in_one_CRISPR/Cas9_LacZ, a CRISPR gRNA plasmid from Lynne Postovit...

...021-00430-1 More resources on the Addgene blog CRISPR 101: RNA Editing with Cas13  RNA Interference ...

...efficiency of up to 20%, comparable to commonly used CRISPR/Cas9 methods for large inserts. However, this system...

...translation in living single cells. However, before CRISPR-Cas9 these techniques were quite tedious and, even...

...temperature conditions, thereby enabling efficient CRISPR-Cas mediated editing for psychrophilic systems ...

...or preparation, and deliver CRISPR components for gene disruption or CRISPRi. While designed to study the...

...Blog Read the Plasmid 101 blog posts Check out CRISPR Meets Synthetic Biology: A Conversation with MIT...

...Service Tips for 1st Time CRIPSR User (By a 1st Time CRISPR User) Tips for Titering Your Lentivirus Preps ...

... In mammalian cells this would be possible with CRISPR/Cas-based genome editing. Selecting a bright fluorescent...

...pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR Renilla EF1α AAV9-CRISPR knockout plasmid. Renilla luciferase is used to...Paul Khavari 109395 EFS-Vector Firefly EF1α AAV-CRISPR knockout plasmid. Firefly luciferase is used to...transfection Sidi Chen 109396 TBG-Vector Firefly TBG AAV-CRISPR knockout plasmid. Firefly luciferase is used to...

... Lentivirus FAQ Learn How Lentivirus is Used in CRISPR Genome-Wide Screening Learn How Lentivirus Has ...

...where you learn biology terms (plasmid, 5-prime, CRISPR, growth strains, vector backbone, etc.) and you...

...transient gene expression in a particular cell type to CRISPR genome editing, optogenetics, and chemogenetics...

...downregulation via RNAi or gene editing/deletion using CRISPR. In some cases, a decrease of the expression efficiency...

...testing in the lab will be greatly facilitated by CRISPR reagents and other specific gene sequences available...

...For instance, through open plasmid sharing, the CRISPR community has created hundreds of useful variants...

... RNA amplification, new toehold switch designs, CRISPR/Cas9-mediated genotyping, as well as our electronic...

...our Viral Vectors Featured Topic Page Learn about CRISPR-AAV vectors Learn about Addgene AAV Quality Control...

...including a bot for biosensor papers, microbe articles, CRISPR papers, and more. Saving papers There isn’t technically...

.... You’re going to engineer the best thing since CRISPR; you are ready to clone! But...how? Cloning is...