We narrowed to 451 results for: crispr

1. Developing a cold tolerant E. coli using specialized chaperones

   Type

   Blog Post

   ...temperature conditions, thereby enabling efficient CRISPR-Cas mediated editing for psychrophilic systems ...

2. Plasmid Tools for Microbiome Studies

   Type

   Blog Post

   ...or preparation, and deliver CRISPR components for gene disruption or CRISPRi. While designed to study the...

3. Plasmids 101: Screening Strategies Used in Plasmid Cloning

   Type

   Blog Post

   ... comes in. Let’s take a look at All_in_one_CRISPR/Cas9_LacZ, a CRISPR gRNA plasmid from Lynne Postovit...

4. Visualizing Protein Turnover In Situ

   Type

   Blog Post

   ...translation in living single cells. However, before CRISPR-Cas9 these techniques were quite tedious and, even...

5. Hot Plasmids - May 2022

   Type

   Blog Post

   ...efficiency of up to 20%, comparable to commonly used CRISPR/Cas9 methods for large inserts. However, this system...

6. Viral Vectors 101: An Introduction to AAV

   Type

   Blog Post

   ...transient gene expression in a particular cell type to CRISPR genome editing, optogenetics, and chemogenetics...

7. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

   Type

   Blog Post

   ...testing in the lab will be greatly facilitated by CRISPR reagents and other specific gene sequences available...

8. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

   Type

   Blog Post

   ...downregulation via RNAi or gene editing/deletion using CRISPR. In some cases, a decrease of the expression efficiency...

9. Developing Lab Management Software for Biology

   Type

   Blog Post

   ...where you learn biology terms (plasmid, 5-prime, CRISPR, growth strains, vector backbone, etc.) and you...

10. Tips for Titering Your Lentiviral Preps

    Type

    Blog Post

    ... Lentivirus FAQ Learn How Lentivirus is Used in CRISPR Genome-Wide Screening Learn How Lentivirus Has ...

11. Botman-Teusink Yeast FP Collection

    Type

    Collection

    ...Markerless Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit Other Addgene Plasmids Bas Teusink...

12. CRISPR Guide

    Type

    Guide

    ...Guide CRISPR Guide Jump To: CRISPR Guide Overview CRISPR Basics Small Edits Large Edits Genome-Wide Screening...Figure 7: Overview of CRISPR transposases Browse Plasmids: CRISPR transposases CRISPR Recombinases and Integrases...screening using CRISPR libraries What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries...References Website CRISPR plasmids and resources at Addgene CRISPR 101 eBook (3rd edition) CRISPR software and... Addgene Blog CRISPR topic page How to design your gRNA for CRISPR genome editing CRISPR 101: Homology.... Figure 1: Overview of the basic CRISPR mechanism Engineered CRISPR systems contain two components: a...generating gRNAs makes CRISPR one of the most scalable genome editing technologies, making CRISPR perfect for genome-wide...

13. Synthetic Biology - Overview

    Type

    Collection

    ...Orthogonal Switches Other Addgene Tools and Resources CRISPR Fluorescent Proteins FREQ-Seq Kit FX Cloning Kit...

14. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

    Type

    Blog Post

    ...For instance, through open plasmid sharing, the CRISPR community has created hundreds of useful variants...

15. Plasmids 101: 5 factors to help you choose the right cloning method

    Type

    Blog Post

    .... You’re going to engineer the best thing since CRISPR; you are ready to clone! But...how? Cloning is...

16. Overcoming the Challenges of Lentiviral Production

    Type

    Blog Post

    ...for transfer plasmids with small inserts (such as CRISPR guides) while a single 72h harvest is best for ...

17. Educational Planarian Tools for Teaching Developmental Biology Techniques

    Type

    Blog Post

    ...Biology with BioBuilder 3D Models for learning about CRISPR Resources at Addgene.org Find plasmids from the...

18. Early Career Researcher Toolbox: Finding Relevant Papers

    Type

    Blog Post

    ...including a bot for biosensor papers, microbe articles, CRISPR papers, and more. Saving papers There isn’t technically...

19. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type

    Blog Post

    ...our Viral Vectors Featured Topic Page Learn about CRISPR-AAV vectors Learn about Addgene AAV Quality Control...

20. Adapting Toehold Switches to Detect Zika Virus

    Type

    Blog Post

    ... RNA amplification, new toehold switch designs, CRISPR/Cas9-mediated genotyping, as well as our electronic...