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Addgene
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  1. DAP arrays for multiplex gene editing

    Type
    Blog Post
    ...higher Cas9-independent off-target editing in comparison with single-site editing. Encouraged by the results...#186715) and hCtRNA_VT (#186716).   Fig. 2: Comparison of previous representative dual-gRNA architectures...

  3. How to Lead a Great Meeting

    Type
    Blog Post
    ...Project Meetings: You do not have to include every person who could potentially be impacted by a project....team meetings, provide agenda sections for each person to add a bullet or two about what they are working...

  4. Advice for Scientists Starting a Lab

    Type
    Blog Post
    ...." She also recommends to seek out advice from seasoned PIs who can offer support. “It is an exciting ...Email. Nothing prepared me for what a PI without a personal assistant has to deal with. My inbox currently...

  6. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ...classification system, CasID, to help facilitate direct comparison of different enzymes. CasPEDIA is meant to be...isn’t always the winner!).   Fig. 3 – Comparison of different Cas enzymes using CasPEDIA. Adapted...

  8. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    ...precision genome editing workflow. Digital PCR comparison to other validation methods As mentioned, these...PMC3773023. 7. Vouillot, L., Thélie, A. & Pollet, N. Comparison of T7E1 and surveyor mismatch cleavage assays...

  13. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    ...For safety and production reasons, multiple plasmids with many components are used to package lab grown...will discuss them together. Gene expression The reason for most viral experiments is to deliver a genetic...

  16. Addgene: The First Twenty Years

    Type
    Blog Post
    ...molecular biology and cloning, since they have reasonable introductions to lots of molecular biology,” ... Rachel Leeson...

  17. Antibodies 101: Fab Fragments

    Type
    Blog Post
    ...world of research affinity reagents, and for good reason. However, sometimes you want a tool that is a little...Variable (scFv). Size: ~25 kDa.   Figure 2: Comparison between a full IgG antibody and common antigen...

  19. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    ...fluorescent protein can be identified by a head-to-head comparison of several fluorescent proteins of the same spectral.... In figure 1 we provide an example of such a comparison between cyan fluorescent proteins using widefield...

  20. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    ...leads with excellence. They are one of the main reasons I have so few worries and can often focus on the... reflect this diversity, remarkably so for a 90 person company. We were and remain ahead of most companies...
Showing: 381 - 400 of 718 results