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  1. 2022 and 2023: Reflections and Looking Forward

    Type
    Blog Post
    ...CRISPR plasmids and 6,000 biosensors plasmids. Our most exciting milestone of 2022, however, is arguably...from 231 countries. Guides and protocols were our most popular content, while our beloved blog received...

  2. Your Top Requested Plasmid in 2015!

    Type
    Blog Post
    ...dug into the data from our repository to find our most requested plasmid in 2015. In line with all of the...year, we're excited to announce that the plasmid most requested from the Addgene repository in 2015 was...

  4. Developing Transferable Skills During Science Training

    Type
    Blog Post
    ...You are finishing your PhD or perhaps you have almost completed a postdoctoral position… or two. You ...of nonacademic science careers is that a team is almost always involved. Demonstrating your ability to ...

  5. Quick, Versatile Plant Transgenesis with GreenGate Plasmids

    Type
    Blog Post
    ... the kit in December, GreenGate includes the six most frequently used elements in plant expression cassettes...generate multi-construct transgenic plants. In the most practical terms, what GreenGate can do is “save ...

  6. Management for Scientists: Seeking Feedback

    Type
    Blog Post
    ...feedback from the team. Successful management can almost be boiled down to one, key concept: Creating a ... style.   Seeking feedback frequently One of the most important paths of information flow is the one that...

  8. Science as Art

    Type
    Blog Post
    ...organism I study, to be able to see it all scales. Most modern biologists focus on one aspect alone. They...requires an awful amount of skill,  time for which most researchers wouldn't have. They are motivated to...

  11. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    ... of information to fit into a single plasmid, so most first-generation binary vectors are quite large ...promoter. Originally isolated from the Mirabilis mosaic virus, the constitutively active M24 promoter has...

  12. SunTag and Fluorescent Imaging

    Type
    Blog Post
    ...display protein aggregation, it’s recommended for most imaging applications. What can you do with SunTag... experiments in their paper, and so can you! In almost any case you would use a traditional FP fusion,...

  13. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    ...Montgomery, or Louis Miller led these sessions. The most beneficial aspect of this course was breaking up...works.  4. Practice writing everyday Overall, the most important instruction the classes taught me was ...

  14. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    ...with clear documentation, so we were excited when Amos Bairoch from the Swiss Institute of Bioinformatics... be very informative. Spoiler alert: HeLa is the most common contaminator line.  For those wanting to ...

  15. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    ...advancing the field.   What is a plasmid? At their most basic level, plasmids are small circular pieces ...that replicate independently from the host's chromosomal DNA. They are mainly found in bacteria, but also...

  16. Antibodies 101: Affinity Tags

    Type
    Blog Post
    ...they are peptides which do not naturally exist in most expression systems that can be added to the N or...downstream applications Below, we describe a few of the most common tags available. Maltose binding protein Maltose...

  17. Management for Scientists: Managing vs. Leading

    Type
    Blog Post
    ...levels of an organization and is also one of the most common criteria required for a promotion. Yet little...fulfilling the requirements of the current task. Most of us start as doers, some become managers and some...

  19. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    ...Wikipedia, accessed 25 November 2013. Author: James Atmos (2). Many CRISPR/Cas moieties carry out these...Wikipedia, accessed 25 November 2013. Author: James Atmos. http://en.wikipedia.org/wiki/File:Crispr.png ...
Showing: 381 - 400 of 759 results