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We narrowed to 449 results for: CRISPR

Showing: 191 - 200 of 449 results

  1. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ...accounts with  Addgene. We now have the incredible CRISPR/cas9 tools at our disposal to to modify the worm...would be useful to our model  so that we can use CRISPR/cas9 to build the mutants, analyse their motor ... crispr...

  2. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    ...publications and funding, bioethics in the age of CRISPR, and the challenges of science becoming increasingly...rapidly changing world of genome engineering via CRISPR.  Though the individual topics are not surprising...scientific world? Would biohackers even be a thing if CRISPR hadn’t made manipulating the genome cheap and relatively...shocking. Mainstream media outlets have addressed CRISPR frequently over the last several years, but lay...scientists themselves.  The sections on optogenetics and CRISPR of course address some of the ethical implications...easy, fast, and cheap distribution of the newest CRISPR-Cas plasmids, has been a key driver of the technology...own books. Multiple books have been written about CRISPR alone, and Zimmer himself has written entire books...

  3. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type
    Blog Post
    ...protocols, and more. Selecting crispr in Guides will return links including our CRISPR guide.  For a closer look...for “CRISPR” plasmids keeps more than half of these. Instead we can navigate first to the CRISPR Resources... search field front and center. Let’s type in “crispr” and see what comes up. Before even clicking the...Type” you can filter by experimental purpose (e.g. CRISPR) Beyond search What can you do if you don’t have...Resources page: The page links to guides, our CRISPR eBook, plasmid kits, and curated pages categorized ...

  5. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type
    Blog Post
    ...Addgene Blog First time CRISPR user? Check out our tips for 1st time CRISPR users Learn how to Choose...Here Brush Up on Your CRISPR Background with Addgene’s CRISPR Guide Page ...resolution, the Xu lab created a dCas9-based CAPTURE (CRISPR Affinity Purification in situ of Regulatory Elements...at hundreds of loci at once (Liu et al., 2020). CRISPR Affinity Purification in situ of Regulatory Elements... crispr...

  6. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    ...enChIP using CRISPR Addgene: Your lab recently developed a retroviral enChIP system using CRISPR technology... molecule technologies such as TAL proteins and CRISPR became available, I was very excited because genome-editing...retroviral enChIP system enables us to express the CRISPR complex much easier in various cells, including...a Retroviral Expression System for enChIP Using CRISPR. Fujita T, Fujii H. PLoS One. 2014 Jul 22;9(7):...molecule-mediated chromatin immunoprecipitation (enChIP) using CRISPR. Fujita T, Fujii H. Biochem Biophys Res Commun.... crispr...

  7. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    ...vector. CRISPR/Cas9-based systems have been developed for generating knockin mice but because CRISPR/Cas9...recombination for precise gene insertion. However, CRISPR/Cas9 systems are more useful for generating knockout...used to engineer knockout mice. ZFN, TALEN, and CRISPR/Cas9 systems can all be targeted to specific regions...can introduce multiple base pair deletions, but CRISPR/Cas9 can also introduce point mutations. While ...Additional resources on the Addgene blog Find our CRISPR blog posts Read blog posts about using Cre-lox ...

  8. The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years

    Type
    Blog Post
    ...our collection. CRISPR/Cas9 - At Addgene, 2013 will be known as the "Year of the CRISPR." The newest genome...many different purposes that we had to create a CRISPR page to keep up with all the new plasmids. So far... far, we have distributed over 10,000 CRISPR/Cas9 plasmids to more than 3,000 labs around the world. ...

  9. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...Bargmann’s keynote speech and in the workshops on CRISPR techniques and new tools for conditional expression...of your gene of interest.  SapTrap Tool Kit for CRISPR Cas9 edits At Addgene, we have the entire SapTrap...toolkit and plasmid assembly line for the creation of CRISPR targeting vectors that each contains a guide RNA...Schwartz and Jorgensen, 2016). Using SapTrap, this CRISPR plasmid can be created in a single tube. You can...Jorgensen. "SapTrap, a toolkit for high-throughput CRISPR/Cas9 gene modification in Caenorhabditis elegans...Addgene blog Learn more about SapTrap Read about CRISPR/Cas9 in C. elegans Browse our Plasmids 101 blog...

  10. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type
    Blog Post
    ...other CRISPR reporters Browse our CRISPR blog posts Resources on Addgene.org Browse the CRISPR guide...the genome, but they’re inefficient compared to CRISPR/Cas9 edits that rely on double strand DNA breaks...converted into BFP (blue fluorescent protein) upon CRISPR/Cas9 homology directed repair (HDR) (Glasser et...Conversion: A Versatile Assay for the Quantification of CRISPR/Cas9-mediated Genome Editing. Molecular Therapy...guide Find CRISPR plasmids for your research ...
Showing: 191 - 200 of 449 results