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We narrowed to 218 results for: ung

Showing: 21 - 40 of 218 results

  1. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ..., 20234Kaludov et al., 2001; Walters et al., 20015Dhungel et al., 2025 Depending on how you count, there...has broad tissue tropism, including liver, muscle, lung, CNS, kidney, retinal, and pancreatic cells (Ahuja... is particularly well-suited for transduction of lung cells via airway administration (Halbert et al.,...identifying a binding receptor published this year (Dhungel et al., 2025)! It can transduce neurons for retrograde...AAV1, AAV8, AAV9 Kidney AAV8 Liver AAV8, AAV9 Lung AAV6 Pancreas AAV2, AAV8 Photoreceptor cells...1359–1368. https://doi.org/10.1089/hum.2008.123 Dhungel, B. P., Xu, H., Nagarajah, R., Vitale, J., Wong...Transduction of Airway Epithelial Cells in Mouse Lungs Compared to That of AAV2 Vectors. Journal of Virology...

  2. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...References Abudayyeh OO, Gootenberg JS, Konermann S, Joung J, Slaymaker IM, Cox DBT, Shmakov S, Makarova KS... Abudayyeh OO, Lee JW, Essletzbichler P, Dy AJ, Joung J, Verdine V, Donghia N, Daringer NM, Freije CA,..., Bhattacharyya RP, Livny J, Regev A, Koonin EV, Hung DT, Sabeti PC, Collins JJ, Zhang F (2017) Nucleic...science.aam9321 Gootenberg JS, Abudayyeh OO, Kellner MJ, Joung J, Collins JJ, Zhang F (2018) Multiplexed and portable...Wolf YI, Gootenberg JS, Semenova E, Minakhin L, Joung J, Konermann S, Severinov K, Zhang F, Koonin EV ...Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin EV, Zhang F (...

  3. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    ...to their own devices. Chytrid fungi (Lillian Fritz-Laylin) Chytrid fungi are useful for the study of basic... basic evolution of fungi and eukaryotes and includes the fungus that is killing frogs all over the world...diverse plants, animals, microbes, viruses, and fungi for which these methods are presently unavailable...world. These are less well known fungi (they are not yeast or mushrooms) but there are over 1,000 species...

  4. Zinc Finger Consortium Reagents

    Type
    Collection
    ...deposited by Zinc Finger Consortium members like the Joung Lab, Voytas Lab, and Wolfe Lab... Consortium Reagents You may also like... Keith Joung Lab plasmids Daniel Voytas Lab plasmids Scot Wolfe...zinc finger technology. Consortium members Keith Joung and Daniel Voytas have deposited at Addgene various...

  5. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    ...possible TF-induced cellular states was generated (Joung et al., 2023). Fig. 1: Barcoded TF library...performed to map TFs to cell fates. (Adapted from Joung et al.)    This atlas linked TF expression to... nominated reference cell types.  (Adapted from Joung et al.)    Putting MORF to work Screens and ...addgene.org! References and Resources  References Joung J. et al., A transcription factor atlas of directed...186 (1) 209-229. doi: 10.1016/j.cell.2022.11.026 Joung J. et al., Genome-scale CRISPR Cas-9 knockout and...

  6. Microbiology Resources

    Type
    Collection
    ...interest, including bacteria, viruses, protozoa, fungi, and more. ...bacteria, viruses, parasites such as protozoa, and fungi. Find plasmids below for the species you work with...Plasmids for Cyanobacteria Cyanobacteria Plasmids for Fungi Species Aspergillus sp. Cryptococcus sp. Plasmids...

  7. TALEN Guide

    Type
    Collection
    ...best-selling kit in Addgene’s history. Dr. Keith Joung’s lab at Massachusetts General Hospital also recently...serial ligation protocol to assemble arrays. Dr. Joung, co-founder of the Zinc Finger Consortium with Dr...Sander JD, Cade L, Khayter C, Reyon D, Peterson RT, Joung JK, Yeh JR. Nat Biotechnol. 2011 Aug 5;29(8):697...

  8. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    ...//doi.org/10.3390/cells8121510 Sung H, Kang SH, Bae YJ, Hong JT, Chung YB, Lee CK, Song S (2006) PCR-based...is quick, efficient, reliable and cost-effective (Sung, 2006). The sensor cell-based method uses specialized... mycoplasmas but also avoid other bacterial and fungal contamination. Proper PPE Since humans are the ... changed regularly as mycoplasma, bacteria, and fungus can reside in these containers. Clean up any media...

  9. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    ...stories Zombie ants Ophiocordyceps unilateralis is a fungus that infects ants and controls their behavior. ...point where the temperature and humidity favors fungal growth. The ants bites down and locks their jaws...its muscles atrophy. After the ant’s death, the fungal fruiting bodies being to sprout out of the ant’...it out of hiding to become exposed to predators. Hungry birds spots the “caterpillar” and then poke out...

  10. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

    Type
    Blog Post
    ...variants derived from analysis of lung adenocarcinoma genomes (Lung Cancer Mutant Collection) or “pan-...variants and genes: High-throughput Phenotyping of Lung Cancer Somatic Mutations. Berger AH, Brooks AN, .... Cancer Cell, 2016. PubMed PMID: 27478040. The lung cancer mutant collection is described in this paper...

  11. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type
    Blog Post
    ...Zhang Lab had described eSpCas9, while the Keith Joung Lab had developed SpCas9-HF1. Both of the enzymes...off-target activity relative to Cas9. Since then, the Joung and Jennifer Doudna Labs used targeted mutagenesis...also increased specificity. Most recently, the Jungjoon Lee Lab used directed evolution to generate Sniper-Cas9...29512652. PubMed Central PMCID: PMC5951633. Lee, Jungjoon K., et al. "Directed evolution of CRISPR-Cas9 ...

  13. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

    Type
    Blog Post
    ...and much more. Now, researchers from the Zhang, Joung, and Doudna labs have improved the on-target specificity... from the Zhang lab at the Broad Institute, the Joung lab at Harvard Medical School, and the Doudna Lab...testing of SpCas9-HF1 Kleinstiver et al and the Joung Lab similarly thought that if they could weaken ...structural knowledge, in collaboration with the Joung Lab, they used targeted mutagenesis to create HypaCas9...

  14. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    ...workshops at the Future of Research Symposium 2014, young scientists are acutely aware of these problems that...new approaches that address the need to train our young scientists for futures both inside and outside of...Paula Stephan, professor of economics at the Andrew Young School of Policy Studies at Georgia State University...diversity of trainee backgrounds, publishing from a young scientist’s perspective, and the many different ...

  15. Hot Plasmids: Summer 2025

    Type
    Blog Post
    ...fluorescent protein mGold2 By Mike Lacy Get your sunglasses — these proteins are bright! Most current yellow...libraries By Mike Lacy Whether you’re planning on lounging poolside or swimming laps, Addgene has pooled ...engineering applications and is especially well-suited for lung cells via airway administration. AAV11 can transduce...reported to target skeletal muscle, kidney, spleen, lung, heart, stomach, and brain. C) Recent studies have...

  16. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    ...linings of certain areas of the body, including the lung, the heart, and the abdomen. Long story short, when...frequently, ingested), it can get into the lining of the lungs or travel to one of the other areas of the body,...of other diseases, such as asthma, pneumonia, or lung cancer. As a result, mesothelioma is often misdiagnosed... from the government as more common cancers like lung (about 224,000 diagnosed this year) or breast cancer...

  17. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    ...Many thanks to our guest blogger Samantha Young. Samantha Young is a Medical Writer with a PhD in reproductive... post was contributed by guest blogger Samantha Young. The use of CRISPR/Cas9 for gene editing has expanded... in scientific content.      References 1. Cho, Seung Woo, et al. "Analysis of off-target effects of CRISPR...

  18. Zhang Lab CRISPR Page

    Type
    Collection
    ...mutated genes in lung adenocarcinoma. Delivery of a single AAV vector ( AAV-KPL ) in the lung generated loss-of-function...complex. Konermann S*, Brigham MD*, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib N, Gootenberg...

  19. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...proteins protect fragile samples during cryo-EM grid plunge freezing By Rachel Leeson Cryo-EM samples can be...glutaraldehyde. Adding RvLEAMshort to the sample before plunge freezing represents a convenient, effective approach...protectant for fragile samples during cryo-EM grid plunge freezing. bioRxiv 2024.02.06.579238. doi: https..., J. M., Adriaens, C., Ramadoss, G. N., Shi, Q., Hung, K. L., Samelson, A. J., … & Weissman, J. S. (2021...

  20. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ...genotyping options. Figure reproduced from Tsai and Joung 2016 with permission. Even though progress has...experiments move closer to the clinic (Tsai and Joung 2016). Table 2: Unbiased Genotyping Options Technique...specificities of CRISPR–Cas9 nucleases”
by Tsai and Joung 2016. Many thanks to our guest blogger Søren Hough...PMC3651036. 15. Tsai, Shengdar Q., and J. Keith Joung. "Defining and improving the genome-wide specificities...
Showing: 21 - 40 of 218 results