...expression vector Cas enzyme promoter can be constitutive (CMV, EF1alpha, CBh) or inducible (Tet-ON); U6 ...efficiency Lentiviral transduction Cas9 and gRNA can be present in a single lentiviral transfer vector or separate...components to make lentiviral particles (for details about lentivirus, see our Lentivirus Guide ) Stable,...lines (for details, see Plasmids 101: Mammalian Vectors ) Transient or stable expression of Cas9 and/or...separate transfer vectors May contain reporter gene (e.g. GFP) or selection marker to identify and enrich...CRISPR elements are inserted into an AAV transfer vector and used to generate AAV particles (for details...oligos and clone these oligos into your desired vector. In many cases, targeting oligos are synthesized...

...Bacteria Culture Collections Lentiviral Plasmids Tetracyline Inducible Plasmids Empty Backbones Bacterial...their options for tools such as empty backbones, lentiviral packaging systems, and luciferase expression.... Top Five by Views Guides CRISPR Guide Lentiviral Guide AAV Guide Optogenetics Guide γ-Retrovirus... CRISPRTwenty Years of Sharing: Addgene's Viral Vector Service...help users get started with tools like CRISPR, lentivirus, and optogenetics — and shared the protocols ...

.../ARS vector (Leu2) that contains dCas9 fused to N 51025 pSLQ1661-sgMUC4-E3(F+E) Lentiviral vector that...bacteria ) contains an anhydrotetracycline (aTc)-inducible dcas9 gene on a p15A vector with chloramphenicol...CRISPR imaging 51024 pSLQ1651-sgTelomere(F+E) Lentiviral vector with sgRNA targeting human telomeres 46913...endogenous TEF1 promoter 46923 pSNR52-sgTET Yeast CEN/ARS vector (Ura3) that contains sgRNA controlled...constitutive sgRNA expression cassette on a ColE1 vector with ampicillin resistance, wherein the N20 can...constructs 46910 pHR-SFFV-dCas9-BFP Human expression vector containing SFFV promoter, dCas9 that is fused to... 46911 pHR-SFFV-dCas9-BFP-KRAB Human expression vector containing SFFV promoter, dCas9 that is fused to...

...Plant Expression Stem Cells Synthetic Biology Tet Inducible Expression Worm Expression Kits Kits are groups...Proteins Protein Expression Synthetic Biology Viral Vectors Communities Find a scientific community that has...Luciferase Optogenetics Chemogenetics Viral Plasmids Lentivirus Adeno-associated Virus (AAV) γ-Retrovirus Adenovirus...

...pooled format into lentiviral transfer vectors (Figure 8B). Libraries come in 1-vector systems, in which...CRISPR libraries What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries are a heterogeneous...heterogeneous population of lentiviral transfer vectors. In most cases, each plasmid contains an individual...Cas9-based genome engineering from a single lentiviral vector. Nucleic Acids Research , 42 (19), e147. PMID...8E). In a 2-vector system, you’ll need to either co-infect with a Cas9-producing lentivirus or use a Cas9...positively-charged grooves on Cas9. gRNA binding induces a conformational change in Cas9, shifting it into...targeting in the double digits. These multiplex gRNA vectors can be used to knock out, activate, or repress ...

...Douglas Kim , GENIE Project Calcium Bicistronic vector expressing GCaMP6 and mRuby2 for imaging neural... Dual- and Triple-Feature Intersectional Single-Vector Delivery of Diverse Functional Payloads to Cells...-X overcomes the calcium channel perturbations induced by the calmodulin in GCaMP. Nat Commun. 2018 Apr...exocytosis and spine enlargement during chemically induced long-term potentiation. J Neurosci. 2006 Feb 15... Mitophagy Dual-emission mitophagy biosensor (lentiviral expression of pH sensitive mt-mKeima) Spatiotemporal...ubiquitin kinase PINK1 recruits autophagy receptors to induce mitophagy. Nature. 2015 Aug 20;524(7565):309-14...

...expression vector Cas enzyme promoter can be constitutive (CMV, EF1alpha, CBh) or inducible (Tet-ON); U6 ...efficiency Lentiviral transduction Cas9 and gRNA can be present in a single lentiviral transfer vector or separate...components to make lentiviral particles (for details about lentivirus, see our Lentivirus Guide ) Stable,...lines (for details, see Plasmids 101: Mammalian Vectors ) Transient or stable expression of Cas9 and/or...separate transfer vectors May contain reporter gene (e.g. GFP) or selection marker to identify and enrich...CRISPR elements are inserted into an AAV transfer vector and used to generate AAV particles (for details...oligos and clone these oligos into your desired vector. In many cases, targeting oligos are synthesized...

...pooled format into lentiviral transfer vectors (Figure 8B). Libraries come in 1-vector systems, in which...CRISPR libraries What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries are a heterogeneous...heterogeneous population of lentiviral transfer vectors. In most cases, each plasmid contains an individual...Cas9-based genome engineering from a single lentiviral vector. Nucleic Acids Research , 42 (19), e147. PMID...8E). In a 2-vector system, you’ll need to either co-infect with a Cas9-producing lentivirus or use a Cas9...positively-charged grooves on Cas9. gRNA binding induces a conformational change in Cas9, shifting it into...targeting in the double digits. These multiplex gRNA vectors can be used to knock out, activate, or repress ...

...Commonly used cloning vectors include Gateway entry vectors and TOPO cloning vectors. If you are looking...combination of a DNA fragment with a plasmid or vector DNA backbone generates a recombinant DNA molecule...specifically engineered plasmids, commonly referred to as vectors, which have become possibly the most ubiquitous...facilitate the cloning of DNA fragments. Cloning vectors tend to be very simple, often containing only a...expression (for the purposes of gene study). Expression vectors must contain a promoter sequence, a transcription...the transcription process to stop. An expression vector can also include an enhancer sequence which increases... amount of protein or RNA produced. Expression vectors can drive expression in various cell types (mammalian...

...188572 Tet-off TauRD (P301L/V337M) MAPT Myc TRE Parkinson's, FTD Franz-Ulrich Hartl 188573 Tet-off Full...Other, non-FP Plasmid Type Mammalian, non-viral Lentiviral AAV Retroviral Bacterial Yeast Other Promoter...19 James Trimmer 129409 Slc1a3-CreERT2 Targeting Vector SLC1A3 PGK Episodic ataxia Walker Jackson 129410...Romet-Lemonne 92200 pINDUCER20-delta-Syn SNCA Myc TRE2 Parkinson's Huda Zoghbi 92201 pINDUCER20-Tau MAPT Myc ...Ataxia Richard Youle 176485 Piggybac-TetO-hNurr1-blast NR4A2 TetO Parkinson's Marius Wernig 176852 UBQLN2...Ronald Hart 190811 pTet-O-APOE-E2-T2A-Puro APOE UbC Alzheimer's Ronald Hart 190812 pTet-O-APOE-E3-T2A-Puro... CMV Ataxia telangiectasia Stephen Elledge 43918 tetO-ALN NR4A2 His, V5 TRE Parkinson's John Gearhart ...