...integrated by the virus. If an oncogene overexpression construct is being delivered, that is a higher risk cargo... GFP. Similarly, if the viral vector carries an shRNA to a gene essential for cellular function, this ...

...AAV vectors to deliver chemogenetic/optogenetic constructs into our cells of interest. Although they have...time until expression, it is possible to use AAV constructs with self-complementing structure (scAAV) (7)... would result in cell death. With several new constructs becoming available, AAVs could be much better...such as microRNAs (miRNAs), short hairpin RNAs (shRNAs), antisense oligonucleotides (ASOs), zinc finger...

...the different vectors used, lentiviral vector constructs have proven very useful due to their ability ...lentiviral vectors fantastic options for delivering shRNA, CRISPR/Cas9 components, and fluorescent sensors...

...Howarth lab created the MBPx-SpyCatcher construct. This construct is used to produce a maltose binding protein...However, the laborious construction of repair templates and guide RNA constructs, as well as the difficult... Human Kinase Domain Constructs Kit, a library of human kinase domain constructs developed through a large-scale... that results in the creation of many E. coli constructs with greatly varied levels of expression. The...can be used to easily create protein expression constructs fused with epitopes or fluorophores, generate...platform for generating both simple and complex DNA constructs Have you ever wished you could design a gene...overexpression experiment, generate the necessary constructs, and complete screening in just 3 days? Thanks...

...promoter is included in the pSico plasmid to drive shRNA expression. Other features that can be included ...those that can are primarily third generation constructs. Lentiviral transfer plasmids are not designed... lentiviral transfer plasmids normally used for shRNA expression? Yes, it is feasible, but first the promoter...within the transfer plasmid must be modified. Most shRNA‐expressing lentiviral plasmids, such as pLKO.1, ... to drive RNA pol III-directed transcription of shRNAs. cDNA expression requires RNA pol II, and thus ...envelope, and transfer vectors, and the resulting construct is packaged into a viral particle, it is not possible...

...transfer plasmid encoding a transgene, sgRNA, or shRNA of interest: The transgene sequence is flanked by...wildtype LTR promoter is no longer present in these constructs. Why do many γ-retroviral plasmids have similar...

...the RNA polymerase. A transcription complex is constructed from the RNA polymerase and several transcription...Polymerase III also transcribes small RNAs, such as shRNAs and gRNAs. Resources Addgene's blog , including...

...interested in. Self-replicating - Once you have constructed a plasmid, you can easily make an endless number...frequently accomplished through expression of an shRNA targeting the mRNA of the gene of interest. These...

...leukoencephalopathy David Root 78523 pFUChW Parkin shRNA Mul1 shRNA PRKN H1 Parkinson's David Chan 78622 pX335...28195 tdp43-EGFP construct2 TARDBP GFP CMV ALS Zuoshang Xu 28196 tdp43-EGFP construct3 TARDBP GFP CMV ALS...28197 tdp43-EGFP construct4 TARDBP GFP CMV ALS Zuoshang Xu 28198 tdp43-EGFP construct5 TARDBP GFP CMV ALS...28199 tdp43-EGFP construct6 TARDBP GFP CMV ALS Zuoshang Xu 28200 tdp43-EGFP construct7 TARDBP GFP CMV ALS...28201 tdp43-EGFP construct8 TARDBP GFP CMV ALS Zuoshang Xu 28202 tdp43-EGFP construct9 TARDBP GFP CMV ALS...28203 tdp43-EGFP construct10 TARDBP GFP CMV ALS Zuoshang Xu 28204 tdp43-EGFP construct11 TARDBP GFP CMV ...Josef Kittler 163858 VPS35 shRNA1 VPS35 Parkinson's Lei Lu 163859 VPS35 shRNA2 VPS35 Parkinson's Lei Lu ...