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  1. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ...deliver mammalian antibiotic resistance (e.g., puromycin, blasticidin), which enables selection of a stable...preparation Appropriate antibiotic for selection (e.g. puromycin, blasticidin) Reagent Preparation DMEM Complete...

  2. Zhang Lab's CRISPR Frequently Asked Questions

    Type
    Collection
    ...marker such as puromycin, you can (also) select for the positive colonies by puromycin selection. You ...transfected cells (for example, plasmid with a puromycin resistance cassette, such as PX459 ). After you...

  3. Lentivirus Plasmids

    Type
    Collection
    ...-pLKO-puro 3rd inducible expression of shRNA; puromycin selection. See plasmid 21916 for Neo selection...19319 pLJM1-EGFP 3rd for EGFP fusion; PGK driven puromycin Sabatini 25895 pLX301 3rd Gateway plasmid for ...

  4. Tetracycline Inducible Expression

    Type
    Collection
    ... shRNA; neomycin selection; plasmid 21915 has puromycin selection TetR On Wiederschain 41393 pCW57.1 Lentiviral...from pCW57.1. rtTA was replaced with tTA, and Puromycin was replaced with Blasticidin selection. Please...

  5. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...and Port pAc-sgRNA-Cas9 dU6 BspQI Transfection Puromycin yes, cut Ji-Long Liu U6-BbsI-crRNA dU6 BbsI Transfection...

  6. Retrovirus Plasmids

    Type
    Collection
    ... For cloning and gene expression; select with puromycin or screen for GFP Bartel 32702 pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE...

  7. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...-Puro Mammalian, piggyBac hU6 pegRNA BsmBI No Puromycin Jacob Giehm Mikkelsen pPBT-PE2-PuroTK-pegRNA_GG...

  8. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...1000000074 (Puromycin) Activation Human Zhang 3rd 3 70,290 SAM v1 - 3 plasmid system 1000000075 (Puromycin) Activation...

  9. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...EGFP, mCherry, iRFP, tdTomato, luciferase, LacZ, puromycin or CreERT2 can be combined with custom-cloned ...a separate reporter gene (EGFP, iRFP, IFP1.4, puromycin, neomycin or luciferase) to create a final lentiviral...

  10. Fujii Lab CRISPR Plasmids

    Type
    Collection
    ...repeat. A retroviral expression vector with the puromycin resistance gene 63591 3xFN-Tel-TAL/pMXs-neo Expresses...

  12. Sequencing Primers

    Type
    Guide
    ...primer Puro-F GCAACCTCCCCTTCTACGAGC 3' end of puromycin resistance gene, forward primer pZIP TCCTTTCCAGCGAGGTTCTA...

  13. Lentiviral Guide

    Type
    Guide
    ... genomes have selectable markers, such as the puromycin resistance gene, conferring antibiotic resistance...
Showing: 21 - 35 of 35 results