AAV Titration by qPCR Using SYBR Green Technology
Type
Protocol
...sequences. Hum Gene Ther Methods. 2012 Feb;23(1):18-28. (Link opens in a new window) PMID: 22428977...Heating plate Pipettors 1–10 µL single channel pipette 20–200 µL single channel pipette 200–1000 µL single...single channel pipette 1–10 µL multichannel pipette 2–50 µL multichannel pipette 20–200 µL multichannel...multichannel pipet to minimize pipetting error and variability Mix samples very well by pipetting back and...multichannel pipette Reagents Universal SYBR Master Mix 2X Primer pair targeting AAV2 ITR ( Aurnhammer et al., ...water Microcentrifuge tubes 96-well optical plate Pipette tips General Considerations Always run standards...that result from a variety of causes, such as pipetting error and sample evaporation. Make the master ...