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Showing: 21 - 40 of 292 results

  6. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...operator. Can be induced by IPTG. Commonly found in pET vectors. Very tightly regulated by the lac operators... repression in the presence of glucose or by competitive binding of the anti-inducer fucose Weaker. Commonly...

  8. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...agent (0.4 mM IPTG is a good start if you’re using a pET vector) and antibiotic for plasmid selection. Remove... for protein overexpression in E. coli, such as pET30a. If working with DNA that is refractory to enzymatic...your resultant plasmid libraries into chemically competent E. coli and analyze transformants for appropriately...order to reduce the chances of some clones being outcompeted by others in liquid culture, dilute and plate...transformation should be performed using a highly competent strain that you can use for long-term library ...probing for cell-binding activity.  First, cut out 20 Petri dish-sized circles of nitrocellulose membrane and...inhibitor cocktail. Line the bottom of 20 empty Petri dishes with thick filter paper and saturate each...

  9. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...available! Interested in purifying Cas9? Check out pET-28b-Cas9-His from Alex Schier's lab, designed for...generated mix of fluorescent proteins, determined by competing recombination events at the genetic level. Upon...

  10. Fluorescent Protein Guide: FRET

    Type
    Collection
    ... compartments pCEP4CyPet-MAMM Cyan Mammalian Expresses mammalian optimized CyPet pCyPet-His Cyan Bacterial...monomeric Venus pCEP4YPet-MAMM Yellow Mammalian Expresses mammalian optimized YPet pYPet-His Yellow Bacterial...Bacterial Expresses CyPet with C-terminal His tag SCFP3A Cyan Mammalian Express a gene of interest fused ...Bacterial Expresses YPet with C-terminal His tag SYFP2 Yellow Mammalian Express a gene of interest fused...fluorescent proteins connected via 5 amino acid linkers pET28CLY1 Peptide linker standard consisting of ECFP and...serine-containing peptide linker (GGSGGS) repeat pET28CLY2 Peptide linker standard consisting of ECFP and...and EYFP connected by 2 GGSGGS repeats pET28CLY3 Peptide linker standard consisting of ECFP and EYFP connected...

  11. Genetic Code Expansion

    Type
    Collection
    ...Bacterial TAG Peter Schultz 48696 pANAP AnapRS E. coli fluorescent AA, Anap Mammalian TAG Peter Schultz 49086...jannaschii p-azido-l-phenylalanine Bacterial TAG Peter Schultz 31190 pEVOL-pBpF p-benzoylphenylalanine ...jannaschii p-benzoyl-l-phenylalanine Bacterial TAG Peter Schultz 48215 pULTRA-CNF tyrosyl-tRNA synthetase...aliphatic diazirine amino acid Bacerial, Mammalian TAG Peter Schultz 50831 pAcBac2.tR4-OMeYRS/GFP* tyrosyl-tRNA...coli various unnatural amino acids Mammalian TAG Peter Schultz 50832 pAcBac1.tR4-MbPyl PylRS M. barkeri... variety of unnatural amino acids Mammalian TAG Peter Schultz 51401 pAM1 NLL-MetRS E. coli azi-donorleucine...tyrosyl-tRNA synthetase M. jannaschii Bacterial TAG Peter Schultz 196486 pcPylRS(Y384F Y306A) PylRS(Y384F ...

  12. CRISPR Plasmids for Genomic Visualization

    Type
    Collection
    ...has been used for dynamic tracking of repetitive and non-repetitive genomic loci, as well as chromosome ...given region. For example, chromosome-specific repetitive loci can be efficiently visualized in living ... targeted sequences in close proximity. A non-repetitive genomic locus can also be labeled by co-delivering...

  13. Structural Genomics Consortium Plasmids

    Type
    Collection
    ...cleavage, AmpR 26093 pET15-MHL EF456738 Hexahistidine tag with TEV cleavage, AmpR 26094 pET28a-LIC EF442785 Hexahistidine...26101 pET28GST-LIC EF456739 GST-tag and hexahistidine tag with Thrombin cleavage, KanR 26096 pET28-MHL ...

  14. CRISPR Guide

    Type
    Collection
    ...have been used for dynamic tracking of repetitive and non-repetitive genomic loci, chromosome painting, and...labeled proteins to the site. Chromosome-specific repetitive loci can be efficiently visualized in living ...multiple target sequences in close proximity. A non-repetitive genomic locus can also be labeled by co-delivering...924–927. PMID: 30377362 Casini, A., Olivieri, M., Petris, G., Montagna, C., Reginato, G., Maule, G., Lorenzin... N. T., Zheng, Z., Gonzales, A. P. W., Li, Z., Peterson, R. T., Yeh, J. J., Aryee, M. J., & Joung, J. .... Cell . 184 (9), 2503-2519.e17. PMID: 33838111 Peters, J. M., Koo, B., Patino, R., Heussler, G. E., Hearne... S., Silvis, M. R., Harden, M. M., Osadnik, H., Peters, J. E., Engel, J. N., Dutton, R. J., Grossman, ...

  15. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...facilitates screening by allowing a multichannel pipette to be used for the remainder of the steps in step...well) to a 96-well PCR plate using a multichannel pipette. Centrifuge PCR plate at 400 x g for 5 min and ...clone present. Resuspend cells in 200 μl of media. Pipette mix to detach cells. Plate 100 μl each into two...

  18. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...Anti-Keyhole limpet hemocyanin [N132A/12R] Keyhole limpet hemocyanin Giant keyhole limpet Mouse IgG2a ...

  20. Qi Lab CRISPR Page

    Type
    Collection
    ...Lentiviral vector that contains sgRNA targeting repetitive sequence of human MUC4 exon 3 46915 pU6-sgGAL4...
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