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  1. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    ...above. In addition, because the moxFPs are highly monomeric, they are an excellent choice for fusions with...Lindsey M. Costantini, and Erik L. Snapp. "Superfolder GFP is fluorescent in oxidizing environments when targeted...Lindsey M., et al. "Cysteineless non-glycosylated monomeric blue fluorescent protein, secBFP2, for studies...
  2. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...-positive assay) as well as stabilization (Nanog-GFP reporter activation and silencing of factor-linked...Ryohei Yasuda have teamed up to develop a novel monomeric cyan-excitable red fluorescent protein (mCyRFP1...shift, long fluorescence lifetime, and a stable monomeric form as determined by the organized smooth endoplasmic...Listen to our mCyRFP1 Podcast Segment New bright monomeric red fluorescent protein - mScarlet! The Dorus ...along with EGFP while retaining an emission spectrum that is easily separable from that of EGFP. This makes...spectra (N- and C-terminal tags for mTagBFP, TagRFPt, EGFP, mVenus, mCerulean3, mKOFP2) and empty vectors that...and combined it with a variety of reporters (i.e. eGFP) and effectors (i.e. DREADDs). The team demonstrated...
  3. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...aptamers able to bind to a small molecule derivative of GFP called 3,5-difluoro-4-hydroxybenzylidene imidazolinone...and may be a better choice for imaging than the monomer. Broccoli has been developed into a split aptamer...
  4. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...dim GFPs The availability of the three dimensional structure of GFP, its brighter mutant S65T-GFP, and...and GFP shRNA. Mice carrying the R26DsRedR; CRUSH-GFP; and Nestin-Cre alleles showed efficient GFP knockdown...identified a family of GFP proteins in the cephalochordate Branchiostoma floridae, named bfloGFPs. They went on...and demonstrate that they can successfully create GFP fusion proteins with a variety of genes across the...enabled scientists to engineer a wide variety of GFPs with diversity in fluorescence brightness, intensity...sensitivity at lower expression levels. APEX2 is a monomeric, 27 kD genetic tag which can be fused to proteins...Cas9 gene editing. Dr. Masahito Ikawa has created a GFP reporter plasmid for scientists to validate the efficacy...
  5. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...are generated by inserting a circularly-permuted GFP into an intracellular loop of various G-protein-coupled...genetically encoded calcium indicator using small monomeric NIR fluorescent proteins (miRFP670nano and miRFP720...
  6. TALEN Engineering

    Type
    Collection
    ...enable users to identify both dimeric TALEN and monomeric TALE transcriptional activator target sites and...TALENs for Endogenous Human Genes TALENs for the EGFP Reporter Gene Additional Plasmids compatible with...
  7. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    ...multi-parametric search queries.  Looking for a bright, monomeric FP published in the last few years with an emission...relating to that specific protein (for instance: EGFP).  Users can “favorite” certain proteins, or create...
Showing: 21 - 29 of 29 results