...kD single heavy domain fragments derived from a camelid antibody called a heavy chain antibody (HCAb). ...

...CRE-ER(TM) - Tamoxifen inducible Hsp68 Mammalian Melton 15508 pBS-OMP-CreFNF OMP targeting vector with ...

... Agarose gel or uMelt analysis dsDNA dye (Sybr)  High No No No Melt curve Hybridization...cycle machine. Melt Curves An advantage of Sybr chemistry is the ability to run a melt curve to validate... up as a smear or band around 30-50 bp. uMelt analysis  uMelt is an online tool that uses the  amplicon...temperature range) to predict PCR melting curves and dynamic melting profiles (Dwight, 2011).  Reference...Interpreting melt curves: An indicator, not a diagnosis. IDT, 2014. Dwight Z; Palais R.; Wittwer CT. uMELT: prediction...prediction of high-resolution melting curves and dynamic melting profiles of PCR products in a rich web...Probes Low Yes, two Yes Yes Agarose gel or uMelt analysis Hairpin probes Low Yes, one Yes...

...https://doi.org/10.1101/354480 Ghanta KS, Mello CC (2020) Melting dsDNA Donor Molecules Greatly Improves ...loci. The first approach – co-CRISPR from Craig Mello’s lab – used inactivation of the unc-22 gene as their...animal, are rescued progeny, which makes screening extremely rapid. This stringent selection allowed me to ... repair templates (Farboud et al., 2019).   The Mello lab also reported that modifying the 5’ modification...They further built on this work, discovering that melting and rapidly cooling dsDNA repair templates dramatically...., 2020). Together, these improvements from the Mello lab resulted in strikingly high editing efficiencies...selectable markers.  CRISPR in C. elegans The Meyer and Mello labs also identified several experimental set ups...

...Nanobodies from camelids have gotten a lot of press for being a promising approach for treatment or prophylaxis...a llama over several days and isolated special camelid antibodies that bind to the spike proteins in cell... immunization has its drawbacks: it requires a camelid animal facility which can be expensive to gain ... like membrane proteins that may unfold due to camelid high body temperature (37.2 - 39.2°C). The Seeger... a single heavy domain fragment derived from a camelid antibody called a heavy chain antibody (HCab). ...Neutralization of Betacoronaviruses by Single-Domain Camelid Antibodies. Cell 181:1004–1015.e15 . https://doi.org...

...fly (Drosophila melanogaster) Another popular model organism is Drosophila melanogaster, or as it is more...such as animals that express fluorescent markers (Mello et al., 1991). Genes can also be easily knocked-...actually rewarded depositors Andrew Fire and Craig Mello a Nobel Prize in 2006. One of  major advantages ...PMID: 28804628. PubMed Central PMCID: PMC5525645. Mello, Craig C., et al. "Efficient gene transfer in C....Nichols. "Human disease models in Drosophila melanogaster and the role of the fly in therapeutic drug ...

...guests, Melina Fan, Kenneth Fan, and Benjie Chen! Melina Fan is a co-founder of Addgene. Melina received... podcast, we sit down with Addgene’s Founders - Melina Fan, Ken Fan, and Benjie Chen. We learn what it...University, where she studied diabetes and metabolism. Melina has worked at Genetics Institute in Cambridge, ...

...Executive Director, Joanne Kamens, and founders, Melina Fan, Ken Fan, and Benjie Chen. Last week, the Addgene...weren’t able to join us in person. Joanne Kamens, Melina Fan, and Ken Fan each spoke about what Addgene ...without whom Addgene could not do what it does.  Melina talked about her grandfather’s humble beginnings... heartfelt portion of the entire night was when Melina and Ken called their parents up to the podium to... great (maybe too great!) time. Ten years ago, Melina Fan had an idea and her family helped bring it ...

...photosensitivity in melanocytes with the goal of understanding both XP and melanoma more broadly. “This...to sunlight and prone to developing metastatic melanomas and other cancers. Joshua Wang (bottom right),...events in the transformation of healthy cells into melanoma cells. They’ll combine induced pluripotent stem... parallel.” Alternating Hemiplegia of Childhood Melodi McSweeney (right), a graduate student at Duke who...

... Many thanks to our guest blogger David Mellor! David Mellor leads the policy and incentive programs ...This post was contributed by David Mellor from the Center for Open Science. In the last decade, researchers...Before coming to the Center for Open Science, Dr. Mellor worked with citizen scientists to design and implement...at https://orcid.org/0000-0002-3125-5888 and @EvoMellor.  ...

...Flybow adapts Brainbow 2.0 for use in Drosophila melanogaster and is under the control of Flp recombinase....downstream of a ubiquitous promoter. Zebrabow is an extremely versatile technology as recombination can be induced...labeling for neural circuit analysis in Drosophila melanogaster." Nature methods 8.3 (2011): 260. PubMed PMID...21297619. Köhler, Corinna, et al. "Mouse cutaneous melanoma induced by mutant BRaf arises from expansion and... and dedifferentiation of mature pigmented melanocytes." Cell stem cell 21.5 (2017): 679-693. PubMed PMID...

... thanks to our guest blogger Fredrik Wermeling! Fredrik Wermeling has a research group at the Centrum ...post was contributed by guest blogger Fredrik Wermeling, leader of a research group at the Centrum for...academic labs, targeting mouse, human, Drosophila melanogaster, and T. gondii are available through www.addgene.org...More information can be found through https://wermelinglab.com/.   References 1. Panda, Sudeepta Kumar, et...

...from them during a panel discussion with Dr. Craig Mello and Dr. Paul Stoffels from J&J. The panel was moderated...the mechanisms of RNAi, the field for which Craig Mello and Andrew Fire won the Nobel Prize. What did Dr...applications. During the panel discussion, Dr. Mello took it upon himself to explain the science to the...can be and how much we have yet to learn. As Dr. Mello said, “Cells have been in the information age for...

...services! Many thanks to Joe Mellor, our guest writer from seqWell Inc. Joe Mellor is the Founder and CEO of...This post was contributed by guest blogger Joe Mellor, Founder and CEO of seqWell Inc. Plasmids and PCR...pieces of synthetic DNA, as well as the advent of extremely fast, cheap and accurate sequencing (“reading”...

...the Association for Creatine Deficiencies! Mark Mellett: IL-36 biology in generalized pustular psoriasis...that would inhibit IL-36 release,” says Dr. Mark Mellett, a senior researcher at University Hospital Zurich...involved in IL-36 release in keratinocytes,” says Mellett. This would help the lab identify therapeutic targets... targets in the future. Congratulations Mark Mellett! Siddharth Prakash: X chromosome genes involved in...

...degradation of MELK. Cell lines were engineered to express FKBP12F36V-MELK and endogenous MELK was inactivated...Featured molecules: dTAG-7 and dTAG-13 Huang et al. MELK is not necessary for the proliferation of basal-...

...needed steps to continue where the guide left off - namely, a blog post devoted to the other affinity reagents...alternative affinity reagents" - everything from camelid antibodies to antibody fragments to tools completely...commercial name nanobodies, are derived from a camelid antibody lacking a light chain, called a heavy ...derived from nature. Single-domain antibodies are extremely small at 12-15 kD but can have weak signals due...

...should take the following steps: 1. Define your timeline Start with your thesis in mind. Imagine the kind...your first (R-level) research grant.  Set your timeline from there, then start identifying the resources...accomplish that goal. 2. Identify the tools in that timeline Avoid writing down prices as you create your list...1. First make decisions based on experimental timelines and specific aims. 2. Then make decisions based...

...will ligate. Subsequent cycling through rounds of melting and annealing allows linear amplification of the...for certain, but may have to do with the slight melting of the 5 ́-terminus during the reaction. This “...incubating the ligation at a temperature near the melting temperature (Tm) of the DNA strands. This selectively...Electrochemiluminescent assay for detection of extremely rare mutations based on ligase reaction and bead...

...the experts in the CRISPR/Cas field, have been extremely helpful for scientists that are looking for more...on “Making Your Own Competent Cells” has been extremely popular, generating many additional questions....and Money by Making Your Own Competent Cells By Melina Fan Plasmids 101: The Promoter Region – Let's...