...expression? Check out our Retro expression vector and Lenti expression vector. Turn it on for just a little ...mechanism to integrate your choice cDNA. Both lentivirus and retrovirus can package cDNAs for genomic ...transiently induce expression? Check out Addgene's Lentiviral dCas9 SAM vector and Piggybac dCas9 SAM vector...promoters and modes of action Check out Addgene's Lentiviral Tet-on and AAVS1 targeted Tet-on transgene vector...Adapted from Nabet et al. Check out Addgene's Lentiviral dTAG cloning vector and SMASh degron tagging ...Addgene website Promoter Guide CRISPR Guide Lentiviral Guide  ...

...cells optimized for viral production (AAVpro or Lenti-X), but any HEK293T cell line should work. This ...checking for protein expression. Sample Data Legend: Lenti-X 293T cells were transfected using 1:1, 1:2, 1:...

...S. pyogenes Puro Zhang lenti sgRNA(MS2)_zeo backbone 61427 Mammalian/Lentiviral BsmBI none S. pyogenes...Yang pLCKO 73311 Mammalian/Lentiviral hU6 none S. pyogenes Puro Moffat lenti sgRNA(MS2)_puro backbone 73795...pyogenes URA3 Davis lenti sgRNA(MS2)_puro optimized backbone 73797 Mammalian/Lentiviral U6 none S. pyogenes...Mammalian/Lentiviral BbsI none S. pyogenes Puro, TagBFP Yusa lentiGuide-Puro 52963 Mammalian/Lentiviral BsmBI...Mammalian/Lentiviral H1-Tet none S. pyogenes Herold lentiGuide-Crimson 70683 Mammalian/Lentiviral hU6 none...50662 Mammalian/Lentiviral See paper none S. pyogenes Blast Sabatini and Lander lentiCRISPR v2 52961 Mammalian...Mammalian/Lentiviral BfuAI none S. pyogenes Puro Wolfe pL-CRISPR.EFS.tRFP 57819 Mammalian/Lentiviral BsmBI...

...protocol on Lentivirus Production provides a general approach for producing lentivirus in Lenti-X 293T cells...production of lentivirus. Purification of this lentivirus results in the pooled library lentiviral prep. B)... for using pooled lentiviral libraries. A) Amplification, validation, and lentiviral generation of a pooled...Usage of a pooled library lentiviral prep for experiments. Pooled library lentiviral preps are generally aliquoted...Pooled Lentiviral CRISPR Libraries- A nice overview of what to consider when using pooled lentiviral CRISPR.... Most of the pooled libraries at Addgene are lentiviral pooled libraries, though other types exist.  ...plasmids requires amplification, verification, and lentiviral production steps before you can start transducing...

...McClure MO, Weiss RA (1997) Sensitization of rhabdo-, lenti-, and spumaviruses to human serum by galactosyl(...target cells which produce lentiviral particles.   Why pseudotype? Lentiviruses and retroviruses typically...related to lentivirus, many retroviral envelope proteins have been pseudotyped on lentiviral particles.... rabies glycoprotein into the lentiviral envelope allows the lentiviral vector to be transported retrograde... Quick Guide to All Things Lentivirus Get tips for titering your lentiviral preps Resources on Addgene.org...Addgene.org: Check out the lentivirus guide Find ready-to-use lentiviral preps at Addgene ...To deliver genes using lentiviral vectors, you need an envelope protein on the virus’s surface and a ...

...plasmid Attila Karsi 105621 Lenti-luciferase-P2A-Neo Firefly EF1α Lentiviral expression of firefly luciferase...fusions using Gateway cloning. Lentival. Mikko Taipale 87078 pLenti6.2-Nanoluc-ccdB NanoLuc® Creation ...fusions using Gateway cloning. Lentiviral. Mikko Taipale 87077 pLenti6.2-Firefly-ccdB Firefly Creation ...transfection Feng Zhang 21471 pLenti PGK V5-LUC Neo (w623-2) Firefly PGK Lentiviral expression of firefly luciferase...luciferase Eric Campeau 21474 pLenti CMV V5-LUC Blast (w567-1) Firefly CMV Lentiviral expression of firefly luciferase...Firefly Ubc Lentiviral expression of firefly luciferase Linzhao Cheng 74444 pLenti.PGK.blast-Renilla_Luciferase...Marcel Bruchez 108542 pLenti-EF1a-Luciferase-IRES-Blast-WPRE Firefly EF1α Lentiviral expression of firefly...

...produce lentivirus? 293T cells are usually used to produce lentivirus. What dictates lentiviral host cell... Guides Lentiviral Guide Lentiviral Guide...FAQ References Glossary Lentiviral Components To increase the safety of lentivirus, the components necessary... below. Third Generation Lentiviral Plasmids 2nd vs 3rd Generation Lentiviral Systems Feature 2nd Generation...generation lentiviral systems? For a full description of 2nd and 3rd generation lentiviruses, please review...What is the difference between a lentivirus and a retrovirus? Lentiviruses are a subtype of retrovirus. From...difference between lentiviruses and standard retroviruses (γ-retroviruses) is that lentiviruses are capable ...

...Bonifacino 173579 Lenti-sgAtm#1/Cre ATM CMV Ataxia telangiectasia Charles Swanton 173580 Lenti-sgAtm#2/Cre ...Venus CMV Lewy body dementia Tiago Outeiro 89643 Lenti-sgAtm/Cre ATM U6 Ataxia telangiectasia Monte Winslow...FusionRed CUP1 ALS Michihiko Kataoka 188460 pKDR-Lenti-CBh.6-SOD1 SOD1 CBh ALS Erik Dent 188572 Tet-off...LRRK2 His T5 Parkinson's Suzanne Pfeffer 208392 Lenti-sgRNA-Cre-GpNLuc-sgmATM ATM Ataxia telangiectasia...Cookson 13318 pLenti6-DEST PINK1-V5 G309D PINK1 V5 CMV Parkinson's Mark Cookson 13319 pLenti6-DEST PINK1-... Cookson 29412 pLenti6-DJ1-V5-E18N PARK7 V5 CMV Parkinson's Mark Cookson 29413 pLenti6-DJ1-V5-C106D PARK7...Cookson 29414 pLenti6-DJ1-V5-L166P PARK7 His, V5 CMV Parkinson's Mark Cookson 29415 pLenti6-DJ1-V5-E18D ...

...descriptions of the lentiviral elements and the 2nd and 3rd Generation Lentiviral Systems can be found...found in our Lentiviral guide. Popular packaging and lentiviral envelope plasmids are listed here. Please...pre-treated with a compound of interest. Lentiviral vectors Popular Lentiviral Transfer Vectors    Expressing your...3rd generation lentiviral vector backbone from the Shih lab. You can find more lentiviral expression vectors...ready-to-use lentiviral prep from the Addgene viral service. CRISPR technology lentiCRISPR v2 (Plasmid ... ~10-fold higher titer of lentiviral particles than the original lentiCRISPR v1. Special note from the...in the genome editing field. Lentiviral Plasmid Kit Multiple Lentiviral Expression System Kit (Kit #1000000060...

...of producing CRISPR lentivirus. As we’ve heard from scientists, producing lentivirus is not always an easy... can make lentivirus production frustrating - a researcher can complete an entire lentiviral production...titering, testing, and providing lentiviral preps of select lentiviral vectors from the repository for ... in lentiviral format. Finally, while we can’t currently produce custom gRNA-containing lentivirus, we... make new lentiviral preps on your own, fear not - anytime you order ready-to-use lentivirus from Addgene...you can use along with lentiviral packaging plasmids to create your own lentivirus stocks. Our research ...re very excited to provide ready-to-use CRISPR lentiviral preps to researchers across the globe. Why provide...

...pSpCas9(BB)-2A-GFP (PX458). lentiCRISPR v2 - This 3rd generation lentiviral transfer vector also from the...promoter. It’s a 3rd generation lentiviral backbone that must be used with lentiCas9-Blast or with cell lines...the last 15 years - the majority of these being lentiviral or CRISPR tools. How many times have these plasmids...Addgene psPAX2  - psPAX2 is a 2nd generation lentiviral packaging plasmid from the Didier Trono lab that...genes. It can be used with 2nd or 3rd generation lentiviral vectors and an envelope expressing plasmid, such...the top requested plasmid of 2015. What makes lentiCRISPR v2 great? It produces a ~10 fold higher titer...CRISPR pooled library screening experiments. Find lentiCRISPR v2.(If you’ve peeked through the rest of the ...

...does it work? LentiCRISPR v2 purpose As its name suggests, lentiCRISPR v2 is a lentiviral transfer vector...the Addgene repository in 2015 was... lentiCRISPR V2   LentiCRISPR v2 originates from Feng Zhang’s lab...designed to have increased lentiviral titer as compared to previous lentiviral vectors. It can be used both...respectively, use lentiCRISPR v2 as the backbone for their many gRNAs. The high titer lentivirus production ... the Addgene Website Visit our Lentiviral Guide Pages Find Lentiviral Plasmids Find CRISPR Pooled Libraries...production afforded by lentiCRISPR v2 is key to its efficient use in screening experiments; large amounts of virus...pooled library screens. Availability and Use LentiCRISPR v2 is available from Addgene both as a single...

...Browse our AAV vector collection. Lentivirus Production Lentiviral preparations ("-LV” catalog items)...Concentrated lentiviral preparations (“-LVC” catalog items) are generated from lentiviral preparations...being frozen. Specifically, lentiviral particles are collected from the lentiviral preparation by precipitation...for in our reported titers. Lentiviral vectors are titered using a lentiviral droplet digital PCR (ddPCR...of the transfer plasmid.   Lentivirus resources Browse lentiviral preps available from Addgene. After...through how we produce both our AAV preps and our lentiviral preps.   Viruses are generated using standard... Cell culture medium (OptiPro SFM) containing lentivirus is first cleared by low-speed centrifugation ...

... of 2017 is the lentivirus envelope plasmid pMD2.G from Didier Trono’s lab! Lentivirus envelope vector... producing our ready-to-use lentivirus preps. You can find our lentivirus production protocol on our website...titering your lentivirus in another blog post. If you need a quick primer on how lentiviruses work, check...2015 and 2016 were CRISPR plasmids), but so are lentiviral vectors, many of which are used to deliver Cas9...promoter. Envelope proteins are a key component of lentiviruses. These proteins coat the virus and help determine...widely used and requested because it enables lentiviruses to infect many different types of cells. This...check out our lentivirus guide page. For more protocols and information, be sure to check out the Trono Lab...

... screens rely on lentiviral systems, a fact supported by the wide array of lentiviral-based CRISPR libraries... while both AAV and lentivirus have low immunogenicity, AAV still beats lentivirus. In mice, AAV is almost...vector delivery methods. Lentivirus has limited packaging options, with most lentiviral vectors being packaged...large packaging capacity and stable expression of lentiviral vectors make them an attractive option. However...made at high titers equal to those achieved with lentivirus but offer an improved safety profile, customization...expression in many mouse tissues. Safety AAV and lentivirus have different safety profiles that often affect...experiments. AAV can be safely handled at BSL-1, while lentivirus requires a BSL-2 setup. This requires a less ...

...use lentivirus to deliver a pooled population of gRNAs to Cas9 expressing cells. Pooled lentiviral CRISPR...generate lentivirus (B). Cas9-expressing cells or wild-type cells are treated with lentivirus containing... Quick Guide to All Things Lentivirus Get Tips on Titering Your Lentivirus Plasmids 101: NGS Quality Control...a heterogeneous population of gRNA-containing lentiviral transfer vectors, each targeting a specific gene...synthesized. Pooled gRNAs are then cloned into lentiviral transfer vectors to create the CRISPR library... concentration that is sufficient to generate lentivirus. Be sure to use next-generation sequencing to...amplification. If you've obtained a ready-to-use lentiviral preparation from Addgene, you can skip the steps...

... cells a virus can infect. Note: While both lentiviruses and γ-retroviruses use the same gene products... well as the viral LTRs, differ. As a result, lentiviral and γ-retroviral packaging plasmids are not interchangeable...using 293T cells This method is very similar to lentiviral packaging methods. The three components described...How do engineered γ-retroviruses differ from lentiviruses? There are a few important differences between...between engineered γ-retroviruses and lentiviruses. First, these viruses were derived from different genomes...genomes (MoMLV and MSCV for γ-retrovirus; HIV for lentivirus). Additionally, γ-retroviruses can only transduce...during mitotic breakdown of the nuclear envelope. Lentiviruses can transduce both dividing and non-dividing...

... Service Suggestion Form. Lentivirus As some of the most popular lentiviral vectors in the repository ... of our new Viral Service, we’re distributing lentivirus (with many CRISPR tools included among the preps...collection. We’ll provide a brief rundown of what lentiviral and AAV preps are available below, but you can... tools, we’re starting the Viral Service with lentiviral preps for the delivery of Cas9 endonuclease, ...we’ve got viruses to help you out. Of course, lentiviruses are used in experiments other than just those...those involving CRISPR and so we also have lentivirus for delivering control shRNAs, GFP, and rtTA from ...with viruses including advice on how to titer lentiviral preps, troubleshoot viral transductions, and ...

...considered safe for clinical applications.     Lentiviral: Lentiviral vectors infect dividing and nondividing...Excisable Cre-lox lentiviral vectors: This approach is the same as using a standard lentiviral vector, but ...transgene reactivation, residual expression Lentivirus Integrating High Low Efficient and Stable Genome...iPSC-transplanted mice. Cre-deletable or Tet-inducible lentiviruses address some of these concerns, but overall ...reprogramming factors driven by one promoter. While lentivirus has similar rates of reprogramming when compared...available from Addgene. Another drawback is that lentiviral vectors insert in different genetic locations...could be affected by the location of insertion. Lentiviral insertion can also disrupt the expression of ...

...Sakuma et al   Multiplexed Lentiviral Expression Cassettes lentiviral transduction One vector expresses...expressing the Cpf1 multiplex system from a lentiviral vector.  Lentiviruses carry a (+) strand RNA copy of the... options for Cpf1 multiplexing: Transfection, lentivirus, and AAV Cpf1’s ability to process its own pre-crRNA... multiple expression platforms: transfection, lentiviral transduction, and AAV transduction.  In most ...Cpf1. This means Cpf1 can bind and cleave the lentiviral RNA, preventing packaging of the virus.  Reversing...of the direct repeat protects the (+) stranded lentivirus RNA from Cpf1-mediated cleavage. This reversing...