Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering
Type
Blog Post
...to avoid MMR:
1) E. coli with inactivated MMR: Using E. coli with inactive MMR is definitely the easier...minutes quickly inactivates the repressor and allows for expression of the recombination genes. After ...confirmation of recombinant clones
If a antibiotic resistance gene has been inserted, recombinants can first...genome engineering and is based on homologous recombination. It allows for direct modification of DNA within...called recombineering - short for homologous recombination-mediated genetic engineering. It can be used... These enzymes then catalyze the homologous recombination of the substrate with the target DNA sequence...nucleotides of homology to the target site for recombination.
Key components of the Lambda Red recombineering...