... et al., 2007). Alternatively, if you prefer Golden Gate assembly, there are a series of plasmids from...and Dinneny, J. R. (2013) ‘A robust family of Golden Gate Agrobacterium vectors for plant synthetic biology...Plasmids from the Tsuyoshi Nakagawa lab are binary gateway vectors and can be used for promoter swapping and...transformant with a single insertion because it will segregate 3:1 in the T2 generation. You also have no control...genotype in T1 than T2. Your transgene should not segregate in T1, but it probably will in T2.
Laura Lee...PMC4426443.
4. Nakagawa, T. et al. (2007) ‘Improved Gateway Binary Vectors: High-Performance Vectors for Creation...