...add overhangs to your insert of interest for Gibson assembly. The steps following primer design and the ...

...gene of interest into the FP-SEC vector using Gibson assembly. This construct will serve as the repair template...

...budget is a concern. The faster methods, like Gibson assembly, are typically more expensive, so you’ll have...

...ligase-mediated method, the more recently developed Gibson Assembly Cloning and Gateway® cloning technologies, ...

...example, DNA assembly methods, such as Gibson Assembly® and NEBuilder® HiFi DNA Assembly, require nick-selective...Plasmid Cloning Techniques Pushing the Limits of DNA Assembly Plasmid Cloning by PCR Resources on Addgene.org...

...Restriction Cloning Try SLIC cloning Learn about Gibson Assembly Additional Resources on Addgene.org Read ...

...extra elements, like a repair template, using Gibson Assembly or restriction enzyme cloning. Streptomyces...are easily inserted using BbsI and Golden Gate Assembly. Either plasmid can be also linearized with XbaI...Microbiome Bacteria with a Toolkit for Modular Assembly of Broad-Host-Range Plasmids. ACS Synth Biol 7...

... recommend using overlap extension PCR and Gibson assembly to piece together the registers. All parts ...