...dedication to resource sharing has led us to develop a searchable and sortable datatable that contains validated...and dCas9-FokI. This table is both sortable and searchable, which can help exclude the data that you are... numeric columns.  The data in any column are searchable and you can search by any part of the data. For...

...in cancer. Yet, the majority of them remain uncharacterized. This represents a considerable barrier to ...Their work culminated in the first functional characterization of over 1,000 mutations, and the group has...are also sensitivity issues when attempting to characterize mutations in a pooled cell population, as mutations... Michael G. Lemieux...

...plasmids is available in an easily searchable format on Addgene’s Michael Davidson Fluorescent Protein Collection...great work of Michael Davidson and his colleagues." Acknowledgments Thank you to Michael Davidson, Michelle...proteins and their use for imaging cell biology, Michael Davidson’s lab at Florida State University has ... the cell, use these markers as your guide. As Michael Davidson told the Tallahassee Democrat in a story...

...installment in our podcast series, we chat with new Addgene Board Member, Michael Koeris. Dr. Koeris did his graduate... capital firm, Flagship Ventures, Koeris helped change Novophage's focus. The company went from developing...pathogens in food products. Along the way, the company changed its name from Novophage to Sample 6. To learn ...

...excited to announce the recipients of this year’s Michael Davidson and Roger Tsien Commemorative Conference...conference of their choice while honoring the late Michael Davidson and Roger Tsien who have played enormous...fluorescent protein tools. This year, we’ve made some changes. In addition to supporting in-person conference...health and disease,” says Aggarwal. This biosensor changes fluorescence based on the release of glutamate ...colleagues Filip Tomaska and Tim Hanson, are characterizing variants of iGluSnFR3 in vivo and have used...be transferred to the roGFP2 cysteine thiols and change its fluorescence properties. The biosensor can ...allow us to better understand how neuron activity changes following opioid withdrawal, providing insight ...

...Susanna Bachle Anion-conducting channelrhodopsins (ChR) are ion channels that can be used to silence the...PubMed PMID: 30166441. MerMAIDs - marine channelrhodopsins for the optogenetic toolbox Article contributed...

...Listen to the photoswitchable protein podcast segment Reversible control of photoswitchable proteins, which...the light- and dark- states of the photoswitchable cPYP, which changes in conformation in response to blue...picking approach to select cells with variant characteristics they wanted, the team identified Archon1, a... Discovery of new protein binder tools for photoswitchable proteins cPYP and AsLOV2 Article contributed... which change in protein conformation in response to light, offers a way to spatially and temporally control...structural knowledge of the proteins. To overcome this challenge, the Uppalapati and Woolley lab developed a phage...bind to only the light- or dark- state of the photoswitchable proteins offers a powerful approach to control...

...Anti-Pan-Nav1 Na+ channel [N97A/31R] Pan-Nav1 Na+ channel Human Mouse IgG2a 114553 Anti-Pan-Nav1 Na+ channel [N230...Anti-Kv2.1 K+ Channel [D3/71R] Kv2.1 K+ Channel Rat Mouse IgG2a 128613 Anti-Kv1.4 K+ Channel [K13/31R] Kv1.4... Kv1.4 K+ Channel Rat Mouse IgG2a 128614 Anti-Kv1.2 K+ Channel [K14/16R] Kv1.2 K+ Channel Human Mouse ...KCNAB2 K+ Channel [K17/70R] Kvbeta2/KCNAB2 K+ Channel Mouse Mouse IgG2a 128616 Anti-Kv1.1 K+ Channel [K20/....1 K+ Channel Rat Mouse IgG2a 128617 Anti-Pan-Kvbeta K+ Channel [K25/73R] Pan-Kvbeta K+ Channel Mouse ...Anti-Nav1.1 Na+ channel [K74/71R] Nav1.1 Na+ channel Rat Mouse IgG2a 128627 Anti-Kv4.3 K+ Channel [K75/41R] ...] Kv4.3 K+ Channel Rat Mouse IgG2a 128628 Anti-Kv2.1 K+ Channel [K89/34R] Kv2.1 K+ Channel Rat Mouse IgG2a...

...GAL1 Huntington's Michael Sherman 1385 pYES2/103Q HTT Flag, GFP GAL1 Huntington's Michael Sherman 8412 pMTH...Friedreich ataxia Michael Ristow 14977 pCINeo-hFX(I154F)-HA FXN HA, AU1 CMV Friedreich ataxia Michael Ristow 14978... ataxia Michael Ristow 14979 pGL3-basic-hFX-promotor(1,3kb) FXN hFXN Friedreich ataxia Michael Ristow ...ataxia Michael Ristow 14981 pGL3-basic-hFX-promotor(2kb, distal) FXN hFXN Friedreich ataxia Michael Ristow...Friedreich ataxia Michael Ristow 14988 pBlue-hFX(I154F)-HA FXN HA, AU1 T7 Friedreich ataxia Michael Ristow 14989...Friedreich ataxia Michael Ristow 14990 pBlue-hFX(T133E)-HA FXN HA, AU1 T7 Friedreich ataxia Michael Ristow 14991...ataxia Michael Ristow 14992 pET28(a)-hFX(I154F)-HA FXN HA, HIS, AU1 T7 Friedreich ataxia Michael Ristow...

...incorporation Michael Jewett 69495 rEc.13.delA 13 amber sites changed to TAA, RF1 function removed Michael Jewett...Methanogenic archaeon Bacterial Ahmed Badran 209739 pAB228i24 G1 PylRS Methanogenic archaeon Bacterial Ahmed...TAG sites changes to UAG, RF1 function removed George Church 48999 C321 all TAG sites changes to UAG George...sites changes to UAG, RF1 function removed Farren Isaacs 87359 C321.∆A.opt all TAG sites changes to UAG...the tRNA is incorporated into the growing peptide chain. To expand the genetic code, modified tRNAs, codons...amber codon) that is recognized by the cognate charged tRNA. Once these plasmids have been introduced ...machinery. To expand the genetic code, 4 major changes to the standard translation machinery are needed...

...mEmerald* Michael Davidson 54491 mCherry-Lifeact-7 Actin Filaments LifeAct mCherry* Michael Davidson 54610...LifeAct EGFP Michael Davidson 55148 mCherry-Tubulin-C-18 Microtubules alpha-tubulin mCherry* Michael Davidson...mApple Michael Davidson 55165 mCherry-ZO1-C-14 Tight Junctions Zonula Occludens-1 mCherry Michael Davidson...pEGFP-GFP11-Clathrin light chain Clathrin-coated vesicles Clathrin light chain GFP Bo Huang 54040 mEmerald-Clathrin...endosomes RAB7 GFP Richard Pagano 12661 DsRed-rab7 WT Late endosomes RAB7 DsRed Richard Pagano 158006 pCMV-mGold-Lysosome-N...endosomes Rab11a GFP Richard Pagano 12679 DsRed-rab11 WT Recycling endosomes Rab11a DsRed Richard Pagano *Fusions...targeted to the same structures as these well-characterized proteins. General Structures The cell contains...

.... You can therefore change the genomic target of the Cas enzyme by simply changing the target sequence...surface-exposed positively-charged grooves on Cas9. gRNA binding induces a conformational change in Cas9, shifting...Fanton, A., Tycko, J., Hinks, M., Chandrasekaran, S. S., Perry, N. T., Schaepe, J., Du, P. P., Lotfy, P., Bassik...approach. Figure 1: Overview of the basic CRISPR mechanism Engineered CRISPR systems contain two components... In this section, we will introduce the basic mechanisms of CRISPR and some of the ways the CRISPR components...efficiency, or flexibility of the Cas enzyme. Basic Mechanisms of CRISPR The requirements for a CRISPR knockout...target DNA. Figure 2: Overview of the NHEJ repair mechanism Cas9 will only cleave a given locus if the gRNA...

..., a conformational change in the sensing region of the construct leads to a change in signal of a circularly... of membrane charges A FRET sensor enables quantitative measurements of membrane charges in live cells...affinities or negative-control constructs used to characterize the sensor's behavior in the target system. ...sensor behavior may differ from the original characterization (e.g. in vitro vs. in vivo , cytoplasmic vs...Improved calcium sensor GCaMP-X overcomes the calcium channel perturbations induced by the calmodulin in GCaMP...GECI) that reports with lifetime and intensity changes A turquoise fluorescence lifetime-based biosensor...calcium sensors (fRCaMP1/2, GECO1/2) The kinetic mechanisms of fast-decay red-fluorescent genetically encoded...

...made up of four protein chains: two large heavy chains and two small light chains. These four domains come... producing antibodies, can change the constant region, and therefore change the isotype of antibodies ...: scFv - Single-chain fragment variables (scFvs) contain only the variable heavy chain and variable light... it is determined by a region called the “heavy chain constant region” or Fc. Different isotypes play ...light chain regions of an antibody. They are small enough to be produced in bacteria (~25 kD), but tend... derived from a camelid antibody called a heavy chain antibody (HCAb). Their small size allows them to... conjugation reaction. Many antibodies can be purchased already conjugated, which can save significant...

...construct expressing mCherry-tagged channelrhodopsin-2 driven by chandelier cell-targeting enhancer Gordon ...213830-PHPeB pAAV_BiCHATe27_ChR2_mCherry AAV construct expressing mCherry-tagged channelrhodopsin-2 driven...mCherry AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by SST interneuron-targeting enhancer...mCherry AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by PV+ basket cell-targeting enhancer...dTomato_nlsdTomato AAV construct expressing dTomato driven by chandelier cell-targeting enhancer Gordon Fishell Viviana...mCherry AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by Lamp5 interneuron-targeting enhancer...mCherry AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by VIP interneuron-targeting enhancer...

...promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades. Michael Bruchas 139505-AAV8...promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades. Michael Bruchas 139505-AAV9...promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades. Michael Bruchas 140135-AAV1...AAV9 pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST Cation channelrhodopsin ChrimsonR fused to mRuby2 fluorophore and ...AAV9 pAAV-hSynapsin- soCoChR-GFP A somatic channelrhodopsin, which enables single cell, single millisecond... pAAV-hSynapsin-FLEX-soCoChR-GFP A somatic channelrhodopsin, which enables single cell, single millisecond...pAAV-CAG-DIO-ChroME-ST-P2A-H2B-mRuby3 Cation channelrhodopsin ChroME targeted to the neuronal soma and proximal...

...-strand break. CRISPR systems, found in 95% of archaeal and 48% of bacterial genomes, are highly diverse...defines 5 types and 16 subtypes based on shared characteristics and evolutionary similarity. These are grouped... aeruginosa . Anti-CRISPR genes employ varied mechanisms to block CRISPR. Some prevent the CRISPR-Cas ...genome. Multiple Cas9 orthologs have also been characterized. Staphylococcus aureus (SaCas9) is smaller than... as guanosine, so the overall effect is an A->G change. Since this method targets RNA rather than DNA,...Xenopus References Barrangou R, Fremaux C, Deveau H, Richards M, Boyaval P, Moineau S, Romero DA, Horvath P....Wiedenheft B, Maxwell KL, Davidson AR. 2015. Multiple mechanisms for CRISPR-Cas inhibition by anti-CRISPR proteins...

...Neurodegenerative diseases are a group of disorders characterized by the progressive degeneration and ultimate... within 3-5 years from symptom onset. Disease mechanisms are still poorly understood but several genes... of nerve cells in the brain. Symptoms include changes in behavior and emotion, uncontrolled movements... -related antibodies including: Anti-KChIP1 K+ channel [K55/7R] Anti-Fig4/Sac3 [N202/7R] Anti-Ankyrin-...window) Rodent Models (Link opens in a new window) Michael J Fox Foundation (Link opens in a new window) A...therapies for those living with Parkinson's today. The Michael J. Fox Foundation has made a number of tools (Link...provided by the foundation can be found on our Michael J. Fox Foundation Plasmid Resource page . CHDI ...

...opsins, which are membrane channels and pores derived from algae and archaebacteria, that can be opened and...widely used Channelrhodopsin-2 (ChR2) from Chlamydomonas reinhardtii . ChR2 is a membrane channel that opens...cations to flow into the cell. Variants of channelrhodopsin have been engineered or identified to increase...microbial opsins, such as the Halorhodopsins, Archaerhodopsins and Leptosphaeria rhodopsins, are pumps that...

... 1 guanine nucleotide exchange factor VAV VAV2 vav 2 guanine nucleotide exchange factor - VAV3 vav 3 guanine... guanine nucleotide exchange factor FLJ40431 ZAP70 zeta-chain (TCR) associated protein kinase 70kDa FLJ17670...lab of Ian Frew . MXS Chaining Kit : From the lab of Pierre Neveu , the MXS-chaining system allows efficient...applications in mammalian systems. The MXS-chaining kit includes chaining vectors with multiple cloning sites...