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Showing: 21 - 40 of 560 results

  1. Viral Production

    Type
    Collection
    ...immunogenic properties of the final product, particularly in large animal studies. Endotoxin contamination...detected. pAAV-Ef1a-mCherry-IRES-Cre was a gift from Karl Deisseroth (Addgene viral prep # 55632-AAVrg). ...being discarded and replaced with a new vial of early passage cells. Approximately 2 weeks post-thaw, ...

  2. Brain Initiative Collection

    Type
    Collection
    ...pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE Expresses optimized PdCO in frame with mScarlet under control of...

  3. CRISPR Guide

    Type
    Collection
    ...Publications CRISPR Overview Class 2 C lustered R egularly I nterspaced S hort P alindromic R epeat (CRISPR...strand separation and cutting at off-target sites. Similarly, SpCas9-HF1 lowers off-target editing through ...target DNA based on the gRNA targeting sequence. Early experiments demonstrated that targeting dCas9 to...genetic screening. Forward genetic screens are particularly useful for studying diseases or phenotypes for...Cas12a (also known as Cpf1) CRISPR C lustered R egularly I nterspaced S hort P alindromic R epeat, a bacterial...2013. Mali P, Yang L, Esvelt KM, Aach J, Guell M, DiCarlo JE, Norville JE, Church GM. Science . 339(6121)...

  4. Zhang Lab CRISPR Page

    Type
    Collection
    ...Delivery AAV - Cas9 Mouse CRISPR ( C lustered R egularly I nterspaced S hort P alindromic R epeats) is ...oligos (design is indicated below) can be cloned scarlessly into the vector before the sgRNA scaffold. The..., and a pair of annealed oligos can be cloned scarlessly into the vector before the sgRNA scaffold. The...

  5. Bacterial Expression Systems

    Type
    Collection
    ...transcription factors, and reporter genes. pCS-PesaRlux 47640 PesaR 3OC6HSL (Quorum sensing molecule) ...The EsaR-responsive promoter can be found in pCS-PesaRlux controlling luciferase expression. pCW-LIC 26098...performing a bacterial two-hybrid (B2H) screen of modularly assembled zinc fingers. Return to Top Do you have...

  6. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...the construct leads to a change in signal of a circularly permuted fluorescent protein (cpFP) or Förster...Neuron. 2020 Jun 20. pii: S0896-6273(20)30432-3. Karl Deisseroth , INTRSECT 2.0 Project Calcium Expression...Neuron. 2020 Jun 20. pii: S0896-6273(20)30432-3. Karl Deisseroth , INTRSECT 2.0 Project Calcium Mitochondrial... voltage sensor to detect neural activity (Ace-mScarlet) A high-speed, bright, red fluorescent voltage...

  7. Antibody Plasmid Collection

    Type
    Collection
    ...Companion to Methods in Enzymology 8, 94–103 (1995). Carlos Barbas Variant of the pComb3 phage display vector...J Immunol Methods. 2000 Aug 28;242(1-2):159-81. Carlos Barbas Vector system for expression, dimerisation...

  8. TALEN Guide

    Type
    Collection
    ...context for each TAL effector within an array, but early studies suggest the likelihood of context having... paper on TAL effectors in Nature Biotechnology early in 2011 and they described their kit for assembling...

  9. TALEN Plasmids and Kits

    Type
    Collection
    ...Gate TALEN 2.0 38142 RCIscript-GoldyTALEN Daniel Carlson The plasmids pC-GoldyTALEN and RCIsricpt-GoldyTALEN...Golden Gate TALEN 2.0 47388 pcDNA3.1-GoldenGate Charles Gersbach These new destination vectors can be used...

  10. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...: An Adaptive Immune System CRISPR (Clustered Regularly Interspaced Short Palindromic Repeat) sequences...26411297 Mali P, Yang L, Esvelt KM, Aach J, Guell M, DiCarlo JE, Norville JE, Church GM. 2013. RNA-guided human...

  11. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ... YouTube . Abstract The prokaryotic clustered regularly interspaced short palindromic repeats (CRISPR)...genomic deletion efficiency mediated by clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 ...

  12. Immunology Research Plasmids and Resources

    Type
    Collection
    ...bA350J20.7 RAET1G retinoic acid early transcript 1G ULBP5 RAET1L retinoic acid early transcript 1L - RELB v-rel...mutated in early stage tumors ARP, MANF, MGC142148, MGC142150 ARMETL1 arginine-rich, mutated in early stage...PA28-gamma, PA28G, REG-GAMMA RAET1E retinoic acid early transcript 1E LETAL, MGC125308, MGC125309, RAET1E2...

  17. Worm Expression Resources

    Type
    Collection
    ...to adult) and easy lab maintenance. Worms are particularly well suited for studies in cell biology, development...

  18. Qi Lab CRISPR Page

    Type
    Collection
    ...bacterial immune system, CRISPR ( C lustered R egularly I nterspaced S hort P alindromic R epeats) pathway...

  19. Plant Plasmids and Resources

    Type
    Collection
    ... in tomato, tobacco, M. truncatula , wheat, and barley - Daniel Voytas. A CRISPR/Cas9 Toolkit for use ...

  20. Open Enzyme Collection

    Type
    Collection
    ... lab costs and overcome supply chain issues, particularly in areas of the world where enzymes are harder...
Showing: 21 - 40 of 560 results