...be easily knocked-down through RNA interference (RNAi) by feeding the worm bacteria expressing specific...of interest (Fire et al., 1998). The discovery of RNAi in the worm actually rewarded depositors Andrew ...

...started with their labs studying the mechanisms of RNAi, the field for which Craig Mello and Andrew Fire...

...hypersaline samples, were engineered and adapted for RNAi experiments for mammalian cell lines. The Turner...

...RNA targeting, with a leg up on the alternatives - RNAi and Cas13. CRISPR-Csm doesn’t degrade proximal RNAs...

... using the pLKO.1 vector, a backbone used by the RNAi consortium for targeting human and mouse genes. ...

...proteins (when knockouts aren’t viable). siRNA or RNAi are small, double stranded RNA molecules that degrade...

...shRNA is used as a negative control for mammalian RNAi experiments.  Find the scrambled shRNA vector. pLKO...

... & CRUSH – rapid & conditional gene silencing in RNAi transgenic mice The Jackson-Grusby lab has designed... in conditional and reversible gene silencing in RNAi transgenic mouse models and embryonic stem (ES) ...transgenic mice, the lab established a dual-color RNAi “sensor” mouse strain in which Cre expression causes...

...Listen to the TRIM-Away podcast segment CRISPR and RNAi have helped researchers alter DNA sequence and RNA...

...bacterial expression, lentiviral, retroviral, AAV, RNAi, luciferase, cre/lox, yeast expression, worm expression...

...translation either by blocking initiation or splicing. (RNAi, in contrast, engages existing gene regulatory mechanisms...

...targeting or genome editing technologies, such as RNAi or CRISPR, non-targeting controls may be appropriate...

...It’s possible to actually silence this gene with RNAi to make a polygamous species monogamous. Anolis ...

...expected protein. Common approaches include using RNAi to knock-down expression of your target or CRISPR...

... Kay. "Future of rAAV gene therapy: Platform for RNAi, Gene Editing and Beyond." Human Gene Therapy ja...

...skyrocketing popularity of CRISPR echoing that of RNAi and iPS cells. Genome engineers continue to work...

... gene-specific plasmids (including reporters and RNAi) for:  NANOG  OCT4 SOX2 MYC KLF4 LIN28 Check ...

...Initial steps in the CRISPR-pooled screening using sgRNAiBAR. Image from Zhu et al., 2019.  Wensheng Wei's...their loop region. After infecting cells with sgRNAiBAR library containing lentiviral vectors the iBARs...the gRNAs and their iBAR sequences. Since the sgRNAiBAR library covers every annotated human gene, it ...number of cells and in vivo settings. Find the sgRNAiBAR library!  Zhu et al., Genome Biology, 2019. PubMed...

...can be used to detect specific phosphoinositides (Varnai and Balla, 2008) (Figure 2). RNA production can...28533371. PubMed Central PMCID: PMC5468623. 11. Várnai, Péter, and Tamas Balla. "Live cell imaging of ...

...polynucleotide chains. J Virol 9(2): 394-396. Lecomte E, Tournaire B, Cogné B, Dupont J-B, Lindenbaum P, Martin-Fontaine...