... not need to obtain more competent cells and retransform. Bacteria on an LB agar plate can be stored at...prepare your plasmid DNA. In the morning, when you retrieve your liquid bacterial culture, take 500 μL of ...

...resolution (the ability to distinguish between two discrete objects). Figure 1 depicts an image of a compound... sample information so that you or others can interpret your images in the future. Conclusion Like any...

...results in cell populations that are more likely to retain stable transgene expression. Other methods of generating...well in sparse/individual cultures due to lack of secreted growth factors, so not every cell type will be...

...position 15-19. Avoid targeting introns. Avoid stretches of 4 or more nucleotide repeats, especially repeated...Incubate for 20-30 minutes at room temperature. g. Retrieve HEK-293T cells from incubator. The cells should...

...end, and the 5’ end of the primer usually has stretches of several nucleotides. Also, both of the 3’ ends...

...cloning can be used anytime you need to add a short stretch of DNA to a plasmid, such as: Changing a Multiple...

...cloning scars"). A “stuffer” sequence allows for electrophoretic separation of linearized vector from the reaction...

...Modification by Annealed Oligo Cloning Add a short stretch of DNA to a plasmid Gibson Assembly Combine overlapping...

...first. Pro-Tip The pH of this solution will drift pretty rapidly upon addition of acid or base. Add only...

...indicates the various controls: Control Ligase Interpretation Uncut vector - Checks viability of competent...

...dropwise. Cap the flasks and swirl 5–10 times to mix. Return the flask to the incubator. Section 3: BCD Feed...

...details on doing diagnostic digests and how to interpret them please see the Diagnostic Digest page. Purifying...

...manufacturer to make sure this is true in your case. Retrieve your molten agar mix from the autoclave. Pro-Tip...

...little chance for mis-priming, so you can use a pretty wide range of annealing temperatures, but you may...

...background staining. Pro-Tip A large shift in the electrophoretic mobility of the bands (sample AR0016 in Figure...

...Takeharu Nagai Magnesium MagFRET-1 Mg2+ sensor, based on Cerulean/Citrine MagFRET: The First Genetically...Campbell Zinc FRET-based zinc sensor for imaging in live cells (CALWY) Genetically encoded FRET sensors to...-2 Zn2+ FRET sensor, cytosolic or ER-targeted eZinCh-2: A Versatile, Genetically Encoded FRET Sensor for...Cytosolic or Nuclear zinc-binding FRET biosensor (Zap) New alternately colored FRET sensors for simultaneous monitoring...Wolf Frommer ATP yAT1.03 FRET-based ATP sensor for yeast An Improved ATP FRET Sensor For Yeast Shows Heterogeneity...cyclic AMP) FRET sensors of cAMP levels for imaging in live cells Fourth-generation epac-based FRET sensors... for Imaging Insulin Secretion A Ratiometric Sensor for Imaging Insulin Secretion in Single beta Cells...

...Collection . Return to top Secretory Pathway The secretory pathway is named for its role in secreting proteins...Endoplasmic Reticulum ER retention signal moxGFP Erik Snapp 68126 ERoxBFP Endoplasmic Reticulum ER retention...38770 pEF.myc.ER-E2-Crimson Endoplasmic Reticulum ER retention signal E2-Crimson Benjamin Glick 36204 ...36204 pmTurquoise2-ER Endoplasmic Reticulum ER retention signal mTurquoise2 Dorus Gadella 137804 ER-mNeonGreen...Endoplasmic Reticulum KDEL sequence mNeonGreen Dorus Gadella 137805 ER-mScarletI Endoplasmic Reticulum KDEL ...Endoplasmic Reticulum BiP ER signal sequence BFP Gia Voeltz 61807 Rtn4a-GFP Endoplasmic Reticulum Rtn4a AcGFP...Endoplasmic Reticulum Sec61 mCherry Gia Voeltz 49154 BFP-Sec61 beta Endoplasmic Reticulum Sec61 BFP Gia...

...PSPS1, PTP, REG RETN resistin ADSF, FIZZ3, MGC126603, MGC126609, RETN1, RSTN, XCP1 RETNLB resistin like ...ULBP4, bA350J20.7 RAET1G retinoic acid early transcript 1G ULBP5 RAET1L retinoic acid early transcript 1L... 3 RAET1N Return to top BCR Signaling B cells mediate humoral immunity through the secretion of antibodies...chromogranin A (parathyroid secretory protein 1) CGA CHGB chromogranin B (secretogranin 1) SCG1 CKLF chemokine-like...FMRFAL NPPA natriuretic peptide precursor A ANF, ANP, ATFB6, CDD-ANF, PND NPPB natriuretic peptide precursor...precursor B BNP NPPC natriuretic peptide precursor C CNP NPR1 natriuretic peptide receptor A/guanylate ...cyclase A (atrionatriuretic peptide receptor A) ANPRA, ANPa, GUC2A, GUCY2A, NPRA NPR3 natriuretic peptide...

...displays enhanced transduction of the retina following subretinal administration and enhanced transduction...transduction and lateral spread in the retina following subretinal injection. It was identified by John ...transduction and lateral spread in the retina following subretinal injection as compared to the parental...delivered directly to the brain parenchyma or subretinal space Serotypes The following serotypes were ...demonstrated to have enhanced transduction of the retina following intravitreal injection in mice and marmosets...trpYF) serotype shows enhanced transduction of the retina following intravitreal injection in mice and macaque...2009. High-efficiency transduction of the mouse retina by tyrosine-mutant AAV serotype vectors. Mol Ther...

...Tamoxifen inducible Retroviral Jacks 24064 MSCV-Cre Cre and EGFP coexpression Retroviral Littman 24333 ins-cre_cryaa-Venus...inducible PGK Retroviral Lowe 33342 LGmCreER (non-self deleting) Cre-ERT2 PGK Retroviral Lowe 33344 pLEW100cre-EP1...pMSCVpuro-Cre Cre 5'LTR Retroviral Ge 34565 pMSCVhygro-Cre Cre and HygroR coexpression Retroviral Ge 34568 WZLneo-Cre...GFP coexpression CAG Retroviral Gage 49054 CAG-GFP/cre Cre-GFP fusion CAG Retroviral Gage 49056 AAV-GFP/...AAV Deisseroth 59701 pRetroQ-Cre-ERT2 Cre-ERT2 -Tamoxifen inducible cmvIE Retroviral Youle 59720 Nanog-CreER...6xHis T7 Bacterial Liu 63704 pRetroX GFP T2A Cre GFP and Cre tight TRE Retroviral Foijer 64769 pTW040 Cre ...neurons Retroviral Luikart 66693 pRubiG-T2A-cre GFP and Cre expression in newborn neurons Retroviral Luikart...