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  1. Plan Your Experiment

    Type
    Collection
    ...Desired Genetic Manipulation Different genetic manipulations require different CRISPR components. Selecting...experimental goal, you are ready to start navigating the different reagents that are available for your particular...targeting promoter elements of target gene Many different activators exist, including the multi-plasmid ...two mutated alleles, each mutated allele may be different owing to the error-prone nature of NHEJ. In HDR... is PCR amplified, PCR products are denatured-renatured, treated with a nuclease that cleaves DNA heteroduplexes...

  2. AAVED

    Type
    Collection
    ... School Dr. Melina Fan, Dr. Susanna Bachle, Dr. Karen Guerin, Dr. Leila Haery, Dr. Meghan Rego, Addgene...Campus Kenton Woodard Penn Vector Core Melina Fan, Karen Guerin, and Leila Haery from Addgene will also be...combination with other technologies to achieve different experimental goals. How does this method compare...this technology or its application? What are some current obstacles that researchers face right now (e.g....an openly shared communication representing a reference for AAV use in neuronal intersectional control...

  3. Neurodegeneration Research Collection

    Type
    Collection
    ...cortical neuron differentiation. Held et al. Cell Rep. 2023 Aug 30. Enhance differentiation to mesencephalic...Collection CRISPR Tools AAV Viral Preps iPSC Differentiation Factors Antibodies Fluorescent Proteins Other...of these are due to a mutation in one of three different inherited genes: Presenilin 1, Presenilin 2, and...al. Nat Methods. 2023 May 4. See More iPSC Differentiation Factors Find plasmids used to transform iPSCs...

  4. COVID-19 Resources

    Type
    Collection
    ...SARS-CoV-2 sequences are now available and more are currently undergoing quality control analysis in our lab...information on these genes, see the links and references below: ACE2 - (Human Angiotensin Converting Enzyme...articles related to SARS-CoV-2 research during the current outbreak: Cell Press Coronavirus Resource Hub COVID...Addgene's Protocol for RNA Extraction Without A Kit References ACE2 - Andersen, KG, et al, 2020, Nature Medicine...2014, J Virol. TMPRSS2 and ADAM17 cleave ACE2 differentially and only proteolysis by TMPRSS2 augments entry...

  5. Deisseroth INTRSECT Collection

    Type
    Collection
    ...Plasmid Collection INTRSECT Implementation Plasmids References The development of powerful molecular reagents...pAAV-Ef1a-Con/Fon/Von-GCaMP6M Cre AND Flp AND Vcre References Fenno LE, Mattis J, Ramakrishnan C, Hyun M, Lee...Conzelmann KK, Staiger JF. 2019. Mapping Brain-Wide Afferent Inputs of Parvalbumin-Expressing GABAergic Neurons...neurons. Preprint (Link opens in a new window) Yu K, Ahrens S, Zhang X, Schiff H, Ramakrishnan C, Fenno L, ...Lazaridis I, Tzortzi O, Weglage M, Märtin A, Xuan Y, Parent M, Johansson Y, Fuzik J, Fürth D, Fenno LE, Ramakrishnan...

  6. All Antibodies

    Type
    Collection
    ...all the antibodies currently available from our catalog. Addgene values transparency and accessibility ...recommended for an application or species. We currently assess western blot, immunohistochemistry, and...should be added to this collection? Check out our current collection of Antibody Plasmids , fill out our ...

  7. Ras Pathway

    Type
    Collection
    ...migration, survival, adhesion, growth, and differentiation. Schematic of Ras signaling cascade that starts...ultimately turn on genes involved in cell growth, differentiation and survival. Ras is essential for regulating...Growth factor receptor bound protein 2 ICMT Isoprenylcysteine carboxyl methyltransferase INSR Insulin receptor...Initiative - at the National Cancer Institute (NCI) References Ras oncogenes and their downstream targets. Rajalingam...

  8. Plasmid Preps: Different Purity, Different Quantities, Different Uses

    Type
    Blog Post
    ...premises we can classify a plasmid preparation in 3 different ways: Transformation grade DNA Cloning grade ... our Plasmids 101 blog posts Learn about the differences between transformation, transduction, conjugation...Resources on Addgene.org Read the molecular biology reference Find plasmids for your research Brush up on molecular...

  9. CRISPR Plasmids - Tagging

    Type
    Collection
    ...throughput over traditional tagging methods. Several different tagging techniques, as well as the plasmids and...plasmids for a CRISPR-based system to add a tag (currently using FLAG) to endogenous proteins. This CRISPR... in each row. If your own gene of interest is currently unavailable, you will need to design and clone...Note that the principle of the PITCh system is different from the conventional HDR-mediated gene knock-...

  11. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ...instructors teaching different types of courses, at any type of institution, and at different points in their...regional hubs, access a teaching mentor, and more. References for teachers Using Bioinformatics and Molecular...

  12. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ... a collection of empty plasmid backbones with different fluorescent tags for you to create fusion proteins...Talley Lambert at Harvard Medical School More References below Jump to: Blue/UV Cyan Green Yellow Orange...fluorescent proteins in the pBAD vector Jump to Top References: Cranfill et al. : Nature Methods July 2016,...

  13. Worm Expression Resources

    Type
    Collection
    ...engineer specific transgenic worm lines. The transparent body of the C. elegans hermaphrodite consists...some great plasmid tools for the community that aren't in the repository? Help us widen our resources ...providing the research community with accurate, current, accessible information concerning the genetics...

  14. p53 Pathway

    Type
    Collection
    ...Background p53 Pathway Plasmids p53 Gene List References Background p53 is a transcription factor and ...sclerosis 2 TSP1 Thrombospondin 1 Return to top References Unravelling mechanisms of p53-mediated tumour...9215639 . Mutant p53 gain-of-function in cancer. Oren, M. and V. Rotter. Cold Spring Harbor Perspectives...

  15. Zebrafish Plasmid Collection

    Type
    Collection
    ... (~3 months), ease of care and breeding, and transparency of the embryo and larvae, it is easy to culture...Ac/Ds transgenic system enabling CRISPR/dCas9-interference (CRISPRi) modulation of enhancers and non-coding... Zebrafish Genome Project - Access the latest reference sequence assembly for the zebrafish genome. Zebrafish...

  16. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ...buffer for vectors delivered directly to the brain parenchyma or subretinal space Serotypes The following serotypes...Shannon Boye’s lab at the University of Florida. References for each serotype can be found below under Citations...following subretinal injection as compared to the parental AAV44.9 serotype. It was developed by Shannon ...

  17. Fujii Lab CRISPR Plasmids

    Type
    Collection
    ...additional information and protocols, check our references for the plasmids or browse the Fujii Lab web ... human IRF-1 promoter for the Sa-Cas9 system. References An enChIP system for the analysis of bacterial...j.bbrc.2018.03.150. PubMed . enChIP systems using different CRISPR orthologues and epitope tags. Fujita T,...

  18. Tetracycline Inducible Expression

    Type
    Collection
    ...neuronal-specific promoter and enhancer options tTA Off Uney References Tight control of gene expression in mammalian...Tn10 encoded tetracycline resistance. Hillen W and Berens C. Annu Rev Microbiol . 1994;48:345-69. PubMed ...transregulators. Krueger C, Pfleiderer K, Hillen W, Berens C. Biotechniques . 2004 Oct;37(4):546, 548, 550...

  19. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...VCP His T5 ALS Graham Warren 14667 pQE9-His-p97(QQ) VCP His T5 ALS Graham Warren 14976 pCINeo-hFX-HA FXN...Parkinson's, FTD Karen Ashe 46905 pRK5-EGFP-Tau AP MAPT GFP CMV Parkinson's, FTD Karen Ashe 46906 pRK5-...Parkinson's, FTD Karen Ashe 46907 pRK5-EGFP-Tau E14 MAPT GFP CMV Parkinson's, FTD Karen Ashe 46908 pRK5...Parkinson's, FTD Karen Ashe 46909 pRK5-EGFP-Tau E14 P301L MAPT GFP CMV Parkinson's, FTD Karen Ashe 46924 YFP-Parkin...mKate2 hsp70 ALS Caren Norden 105971 pME mKate2-dynactin1-811 DCTN1 mKate2 ALS Caren Norden 106093 pFRT_TO_FlagHA_TIA1...

  20. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...IgG2a 206555 Anti-Alpha-2C adrenergic receptor [N330A/80R] Alpha-2C adrenergic receptor Human Mouse IgG2a... Mouse IgG2a 220412 Beta-2 adrenergic receptor [N430/30R] Beta-2 adrenergic receptor Mouse Mouse IgG2a...Plasmids scFv Plasmids Nanobody Plasmids Protocols References The UC Davis/NIH NeuroMab Facility (Link opens...scFv] N324/2 Rem2 Rat Mouse 219492 Anti-Alpha-2C adrenergic receptor scFv [N330A/80 scFv] N330A/80 Alpha-...NeuroMab Protocols (Link opens in a new window) References Andrews NP, et al. 2019. A toolbox of IgG subclass-switched...
Showing: 21 - 40 of 1014 results