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Showing: 21 - 40 of 81 results

  1. Antibodies 101: Epitope Availability

    Type
    Blog Post
    ...If you work in a lab that regularly does immunoimaging, there’s likely a large collection of antibodies...frustration when designing and troubleshooting immunoimaging assays. It can also elucidate information about...

  2. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ...variability in the strength of staining and background noise. If you do use different lots of your polyclonal...antibodies produce a higher level of background noise compared to monoclonal or recombinant antibodies...

  4. CRISPR in the Clinic

    Type
    Blog Post
    ...functional correction of the gene in clonally expanded organoids. This work, and many others, pushed the field ...of CFTR by CRISPR/Cas9 in Intestinal Stem Cell Organoids of Cystic Fibrosis Patients." Cell Stem Cell. ...

  6. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ...quantify specific amplicons.  Sybr has high background noise, due to the large amount of dsDNA in most starting... more sensitive than Sybr, with less background noise, and is not affected by AT content or length of ...

  8. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...improvements. The new variant has increased signal-to-noise ratio and better ability to record subthreshold ...vitro and in vivo, including in human retinal organoids and live mice. Importantly, REVeRT avoids the ...

  9. The Challenges of Cell Culture

    Type
    Blog Post
    ...Required for Acquisition of Anoikis Resistance and Metastatic Potential in Adenoid Cystic Carcinoma, Up-Regulation...

  10. Lentivirus Plasmids

    Type
    Collection
    ...marker. See plasmid 14749 for Thy1.1 selection. Benoist and Mathis 21915 Tet-pLKO-puro 3rd inducible expression...

  11. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...al. 2022). This new version has higher signal to noise ratio and is better localized for synaptic glutamate...requires a probe with fast kinetics and high signal/noise ratio. Here, Takeharu Nagai and colleagues present...

  13. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    ...brighter a fluorophore, the better the signal-to-noise ratio, and the better the detection of the target...brightness makes it easier to distinguish a signal from noise; therefore, it is recommended to use brighter fluorophores...

  14. Which Fluorescence Microscopy Techniques is Best for Me?

    Type
    Blog Post
    ...us. Without contrast, objects simply vanish into noise. Due to its unrivaled contrast, fluorescence imaging... By collecting more photons, a higher signal to noise ratio can be achieved (6). Super-resolution techniques...

  15. Rett Syndrome

    Type
    Collection
    ... receptor Co-Repressor 1/Silencing Mediator of Retinoic acid and Thyroid hormone receptor (NCoR/SMRT) ...

  16. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...to see if lower concentrations reduce background noise. Temperature and time You may need to optimize your... used solutions for washing. If your background noise is high, you can consider adding a detergent like...
Showing: 21 - 40 of 81 results