...Recombinase-deficient strains like Stbl2, Stbl3, and NEB stable E. coli reduce the rate of recombination in...

...DNA efficiently at the end of a linear piece (see NEB for more information). Thus, we recommend that you...

... recombinant-deficient bacterial strains such as NEB Stable, Stbl3, JC8111, SURE2, or XL10-Gold will help...

...Resources Review of restriction modification systems NEB's Resource describing Dam and Dcm Methylases Discovery...

...ligation mix in different bacterial strains (stbl2, NEB stable, etc.), and/or use a different temperature...

...blog High Complexity Golden Gate Assembly with NEB’s DAD Interview with Nicola Patron on MoClo Plasmids...

...however, there are a few popular lab strains, such as NEB Stable, JM109, XL1 Blue or XL10 Gold, that are F’...

...reagents. Thermo Fisher Scientific Inc., Origene, and NEB® are all good places to look for some helpful kits...

...questions (besides Addgene, I know both Abcam and NEB have Ph.D.s answering emails and phones). We can’...

... plasmids with inverted terminal repeats. We use NEB Stable to transform and prep high quality DNA that...

...bacteria designed to minimize rearrangements such as NEB Stable or use a standard strain, like DH5α, grown...

...stability, such as some of our viral vectors, in NEB Stable cells. Resource information: This section ...

...time. Resources and References New England Biolabs (NEB) (Link opens in a new window) sells DNA Assembly ... also sell ET SSB (Link opens in a new window) . NEB has other resources, such as a primer design tool...Assembly kits and master mixes, including NEBuilder HiFi (Link opens in a new window) and Gibson Assembly (Link...

...expressed in SHuffle T7 Express competent E.coli (NEB) using IPTG induction. To biotinylate the nanobodies...

...viral production are propagated in the endA-mutated NEB Stable strain of E. coli. In addition, plasmids are...

...for storage and distribution at Addgene are DH5α, NEB stable, ccdb survival, and Pir1. The majority of ...

...to your own research needs. Other companies like NEB and ATCC can provide you with additional validated...

...al., 1981). E. coli strains such as DH5α, HB101, NEB Stable, Stbl3, etc., are recA- mutant strains, and...

...information about restriction enzymes check out NEB's website . Insert The insert is the gene, promoter...(lac- proAB) [F traDelta36 proAB lacIqZDeltaM15] NEB Stable New England Biolabs F' proA+B+ lacIq ∆(lacZ...

...Cloning High Complexity Golden Gate Cloning with NEB’s DAD New MoClo basics blog ...