...presence of specific molecules or other factors. Most often, a conformational change in the sensing region... astrocyte calcium signaling in the hippocampal mossy fiber pathway. Neuron. 2014 Apr 16;82(2):413-29....from ATP-Mg Hydrolysis Contributes to Mitotic Chromosome Condensation. Curr Biol. 2018 Feb 5;28(3):444...intracellular environment of S. cerevisiae . The MOSAIC Kit from the Cohen laboratory includes 19 lentiviral...

...exhibit almost unidirectional insertions, but are more prone to off-target insertions. CASTs are most often...

...Parasites such as Plasmodium (malaria), Toxoplasma (toxoplasmosis), Trypanosoma (African sleeping sickness), Cryptosporidium...homology-directed repair (HDR) instead of NHEJ. In most experimental systems, HDR occurs at a much lower...

...Selective Actuator Modules (PSAMs). DREADDs are the most widely used chemogenetic receptors and are G-coupled...recently developed class of tools are Luminopsins (LMOs), or luminescent opsins: fusion proteins of a light-emitting...

...by ordering cloning grade DNA for over 200 of our most popular plasmids!...but helps to inform us of which plasmids would be most helpful to requesting scientists. Return to top ...

... provides information for the assembly standards most commonly used by part registries, including the ... Cloning Techniques Guide for an overview of the most common methods. BioBrick BioBrick-2 BglBrick Silver...

...range of dividing and non-dividing cell types with almost 100% efficiency. Unlike lentiviruses or other retroviruses...Adenoviruses can be produced by several methods, the most common of which involves homologous recombination...

...Cancer Impact p53 is the most frequently mutated gene (>50%) in human cancer; most of these mutations are...

...region of DNA in the genome of your favorite species almost as easily as correcting a typo in the latest draft...make arrays from 12-31 repeats. Perhaps one of the most appealing features of the TAL effector arrays is...

...small GTPase and constitutively active Ras is the most common oncogene in human cancer....leading to cancer. Constitutively active Ras is the most common oncogene in human cancer, found in around...

...upload. Currently supports: Human, mouse, rat, marmoset, pig, chicken, frog (X. tropicalis and X. laevis...human, rat, rabbit, pig, possum, chicken, dog, mosquito, and stickleback. E-CRISP Identifies gRNA target...

... to choose which site is likely to result in the most efficient on-target cleavage. The gRNA target sequence...nature of NHEJ. In HDR gene editing experiments, most mutated alleles will not contain the desired edit...

... The beauty of CRISPR is that it’s adaptable to almost any model system or biological topic, and you don...Pederson T. 2015. Multicolor CRISPR labeling of chromosomal loci in human cells. Proc Natl Acad Sci U S A...

...determining subcellular localization. One of the most common is to use fluorescence microscopy to compare...junctions, gap junctions, tight junctions, and desmosomes. Browse or search the table below to find markers...

...work in your lab. Find the FLINC biosensors here. Mo et al. Nat Methods. 2017. PubMed PMID: 28288122. ...

...Link opens in a new window) at the NIH. Reference Ramos et al. 2021. Tackling neurodegenerative diseases...

... regulating the growth of the cell. p53 p53, the most frequently mutated gene in human cancer, is a transcription...

...homology-directed repair (HDR) instead of NHEJ. In most experimental systems, HDR occurs at a much lower...

...homology-directed repair (HDR) instead of NHEJ. In most experimental systems, HDR occurs at a much lower...

...belongs to the Class 1 family of CRISPR systems, the most abundant type found in bacteria and archaea. While...