...2019). This dual lentivirus system pairs ubiquitous expression of sgRNA(s) and mCherry (vector 1) with ...by the Day Lab, who developed neuron-optimized lentiviral vectors for in vitro and in vivo CRISPRa (Savell...neuronal physiology. The Day Lab’s neuron-optimized lentiviral vectors can be found here. High-throughput screening...Cas9 activators can be delivered to the CNS by lentivirus, which has a larger packaging capacity than AAV... such as EF-1α and CAG. Figure 2: Dual lentivirus system with ubiquitous expression of sgRNA (top...

...module and the SspB-mCherry-p60 katanin module, which are also available as lentiviral plasmids if you want... activity. EB3N-VVDfast-iLID (anchor) and SspB-mCherry-p60 (katanin) are recruited to microtubules under...

...expression Chen 52114 pMSCV-IRES-mCherry FP MSCV A bicistronic IRES-mCherry containing retroviral plasmid...Resources: γ-Retrovirus Guide Biosafety Guide Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook...

...facilitates endosomal escape. Combining Cas9-PAGE with lentiviral sgRNA expression yielded editing of 80–90% in...assess Cas9-PAGE editing with viral sgRNA targeting mCherry reporter (mChe), with co-incubation with assist...

...The pgRNA_humanized also contains a CMV-puro-t2A-mCherry expression cassette, useful for selection or fluorescent...CRISPR imaging 51024 pSLQ1651-sgTelomere(F+E) Lentiviral vector with sgRNA targeting human telomeres 46913...dCas9 fused to N 51025 pSLQ1661-sgMUC4-E3(F+E) Lentiviral vector that contains sgRNA targeting repetitive...

...FUGW-APPwt-T2A-mCherry APP mCherry UbC Alzheimer's Ronald Hart 190804 FUGW-APPSwe-T2A-mCherry APP mCherry UbC Alzheimer's...-T2A-mCherry APP mCherry UbC Alzheimer's Ronald Hart 190807 FUGW-APOE-E2-T2A-mCherry APOE mCherry UbC ...T2A-mCherry APOE mCherry UbC Alzheimer's Ronald Hart 190809 FUGW-APOE-E4-T2A-mCherry APOE mCherry UbC ...115331 pLNCX2-mCherry-CHMP2B CHMP2B mCherry CMV ALS Sanford Simon 115333 pLNCX2-mCherry-CHMP2B-siRNAres...Lazarou 119683 pBMN mCherry-OPTN(D474N) OPTN mCherry ALS Michael Lazarou 119684 pBMN mCherry-OPTN(F178A/D474N...Calamai 196704 mCherry-APP-mGFP APP GFP, mCherry CMV Alzheimer's Martino Calamai 196705 mCherry-APPP1-mGFP... CMV Parkinson's Richard Youle 23956 mCherry-Parkin PRKN mCherry CMV Parkinson's Richard Youle 23966 pTreTight-Htt94Q-CFP...

...expression of fluorescent NADH/NAD+ biosensor Peredox-mCherry in plants In Vivo NADH/NAD+ Biosensing Reveals ... Mitophagy Dual-emission mitophagy biosensor (lentiviral expression of pH sensitive mt-mKeima) Spatiotemporal...MOSAIC Kit from the Cohen laboratory includes 19 lentiviral plasmids for a variety of fluorescent sensors...