... Traditionally, they have been made as either polyclonal antibodies or monoclonal hybridomas, but those...those techniques have several drawbacks. Polyclonal antibodies produced in animals may be variable among...& Plückthun, 2015a).   Figure 1: (Top) Polyclonal antibodies are produced in animals and consist...Recombinant antibodies offer numerous advantages over polyclonal antibodies and traditional hybridomas.  First...

...cloning capacity of ~8 kb, so either a single polycistronic vector or multiple single-gene vectors can be...retroviral vectors. This makes it possible to use a polycistronic cassette that expresses multiple reprogramming...factors can be delivered either as a single polycistronic transgene or with four different adenoviruses... ~14.5kb minicircle expressing a 2A peptide polycistronic cassette comprised of OCT4, SOX2, LIN28, NANOG...

... CRISPR knockout library Article contributed by Alycia Bittner CRISPR pooled libraries are popular tools...ApoB-LPs) are indirectly measured through their triglyceride or cholesterol contents (what is commonly called...and indirect measurement of ApoB-LPs through triglyceride/cholesterol does not provide a clear view of...

...targets human glycosyltransferase genes and other proteins related to cellular glycosylation. Get the library... PAM. Get the plasmids at Addgene The Human GlycoGene CRISPR Library is a new lentiviral CRISPR library...

...protein folding, formation of disulfide bridges, glycosylation and acetylation to create functional, stable...systems enable high expression of very complex, glycosylated proteins that cannot be produced in  E. coli...optimized. Yeast cells are also known for hyperglycosylating proteins which may be an issue depending ...

... Addgene - DNA sent from depositors, bacterial glycerol stocks, prepped DNA ready for our quality control...storage on Thursday. The lab prepares bacterial glycerol stocks on Fridays. From each overnight culture... to transformed cells and finally to bacterial glycerol stocks that are used to fulfill orders.  The barcoded...

...repair mediated by uracil glycosylase (Figure 1). The presence of a uracil glycosylase inhibitor (UGI) minimizes...promoter driven expression system. We use a single polycistronic tRNA-gRNA (PTG) gene system to clone multiple...

...positively charged polycation, polybrene. Interestingly, one study found that alternative polycations such as DEAE-dextran...efficiency of lentiviral vectors: effect of sera and polycations.” Molecular Biotechnology 53.3 (2013). 308-14...

...dUTPase and uracil deglycosidase. dUTPase prevents the buildup of dUTP and uracil deglycosidase removes uracil...uracil-labeled template strand is degraded by uracil DNA glycosylase (UDG) and Exonuclease III (ExoIII) (step 4). ...

...Single polycistronic lentiviral vector for the expression of human Oct4, Sox2, and Klf4 Polycistronic lentiviral...27(5):1042-9. Townes Lentivirus Human Single polycistronic lentiviral vector for the expression of human...(8):1221-9. Cibelli Lentivirus Human Single polycistronic, doxycycline-inducible lentiviral vector for...Non-integrating mini-intronic plasmid (MIP) polycistronic expression of human Oct4, Klf4, Sox2, c-Myc ...episome Human Non-integrating EBNA1-mediated polycistronic expression of human Sox2, KLF4, L-Myc, Lin28...Replicating EBNA1 episome Human Non-integrating polycistronic expression of human Oct4, Klf4, Sox2, c-Myc,...episome Human Non-integrating EBNA1-mediated polycistronic expression of human Oct4, Sox2, Myc, Klf4, BCL2L1...

...or repair. Fusing an inhibitor of uracil DNA glycosylase (UGI) to dCas9 prevents base excision repair,...producing a double strand break. UGI = uracil DNA glycosylase. Created with BioRender.com. The BE3 system...byproducts likely resulted from excision by uracil N-glycosylase (UNG) during base excision repair. Adding a second...

...distribution funds our operation. Antibodies are glycoproteins that are part of the humoral immune system. ...against a target antigen. This contrasts with polyclonal antibodies, which are mixtures of monoclonal ...

...biorender.com.    Since antibodies, especially polyclonal antibodies, can vary lot-to-lot, this range needs...this starting point. You can also consider using polyclonal or recombinant multiclonal antibodies to increase... time Not enough antibody binding sites Polyclonal or recombinant multiclonal antibodies Protein...

...as glyphosate, in water. He aims to leverage a glycine oxidase reaction to indirectly detect glyphosate...will co-express together with a plasmid encoding glycine oxidase. The goal is to determine potential water...

...one epitope. (It can sometimes be an issue with polyclonal antibodies, so it's good to keep epitope availability...Antibodies 101: Monoclonal Antibodies Antibodies 101: Polyclonal Antibodies Antibodies 101: Introduction to Antibodies...

...for antibody production have several drawbacks. Polyclonal antibodies produced in animals may be variable...potential for further engineering as compared to polyclonal or monoclonal antibodies. You can learn more ...

...single colony and do not subculture directly from glycerol stocks, agar stabs, or liquid cultures. Freshly...higher plasmid yield that colonies streaked from a glycerol stock. Incubation for 12-16 hours tends to give...

...Cassettes transfection gRNAs expressed from a polycistronic transcript  Requires co-transfection of Cas9...Cassettes transfection gRNAs expressed from a polycistronic transcript Requires co-transfection of Cas9  ...

...plasmids, such as VSV-G (Vesicular Stomatitis Virus glycoprotein), however, may be used across both systems.  ...Envelope VSV-G Vesicular stomatitis virus G glycoprotein; Broad tropism envelope protein; Pseudotyped...

...incubate the sample with your antibody.  Both polyclonal and monoclonal antibodies can work well in ChIP...Antibodies 101: Immunoprecipitation  Antibodies 101: Polyclonal Antibodies Antibodies 101: Introduction to Antibodies...