...library allows researchers to reconstruct the full individual sequence. Traditional NGS workflows are limited...costly library prep, as each sample requires an individual library. The plexWell™ workflow is a sequential...continuing to process 100s to 1000s of samples individually, plexWell™ reduces every 96 well plate to a ...often arises from the fact that large numbers of individual input DNA samples (e.g. a collection of 96 plasmids... prep methods because the yield or quality of individual libraries can be influenced by the quantity or...usually include a separate normalization step where individual input DNA samples are brought in line with each...normalization, it is also often the case that individual libraries prepared from multiple samples will...

...perfect for my next experiment!” but were unable to find the plasmid at Addgene? Well, we’re here to help... we’re asking you for plasmid suggestions. Can’t find a plasmid you’re looking for in the repository? ...name of the plasmid and a publication where we can find the plasmid. While we cannot guarantee that everyone...

... understand. I did, however, find it very hard to speak with individuals who had very strong beliefs in...applied for a role in Industry. I wanted to get "a real job" and roles in Industry do tend to pay better... (GLP) (important buzzwords in industry) as well as skills in industry positions. There were, however... a little different. Industry and academic jobs do exist, but working in industry just wasn't for me, ...roles in the field require experience. Working in industry: Microbiology On returning from Ghana at the beginning...personal bank loan. I saved money from my role in Industry and took evening work to help cover the costs....growing. These factors left me optimistic that I would find a genetics related role soon after finishing my ...

...many find it helpful to obtain support and ideas from those who seem to be managing. Being reminded of ...time. #1 Thou shalt find enjoyment in your work If you are happy at work, you will find balance. If you enjoy... you are not happy at work, you probably need to find a new job. If you are miserable at home or work,...better. #8 Thou shalt enthusiastically foster independence Your partner can make his or her own dinner ...assignments, the better. In my opinion, a level of independence is better for them anyway. You will worry less...the time my eight-year-old daughter vehemently reminded her doctor that she was allergic to penicillin...

...other great tips for finding mentors in this recent blog 5 Strategies for Finding Your Ideal Mentor. One...describe some of the many ways you can approach finding someone to give you advice and guidance. Second... the more potential mentors you will identify. Finding potential mentors is one of the many reasons to...group mentoring program in many of its chapters. Find out what other groups have going on locally. Your...good coach can make a big difference, if you can find one. Create your own peer mentoring circle. This..., “This was really helpful, perhaps you wouldn’t mind doing it again next month?” Some mentoring relationships...

... increase its stability and DNA binding activity, and allow it to bind transcriptional co-activators and...not indicate a specific relationship. p53 Pathway Color is used for clarity and does not indicate a specific...and DNA-damage-inducible; alpha, beta, or gamma IGF-BP3 Insulin-like growth factor binding protein 3 KAI...protein p53 regulated apoptosis inducing protein 1 p53R2 p53 inducible, ribonucleotide reductase M2 B ...apoptosis effector PIDD p53-induced death domain protein 1 PIGs Etoposide induced 2.4 PIRH-2 Ring finger and...these mutations are missense mutations in the DNA-binding domain, often at one of six hotspot residues. These... cancer. p53 Pathway Plasmids Click on a name to find available plasmids for the gene, or browse the gene...

...Find intersectional expression plasmids that combine multiplexed recombinases, including Cre, Flp, and...Standard Operating Procedure (Link opens in a new window) with general principles for working with INTRSECT...experimental design. How-to guide (Link opens in a new window) for the molecular design and testing of novel ...plasmids. Online spreadsheet (Link opens in a new window) of Flp-recombinase-expressing transgenic mouse...inhibitory opsins , genetically-encoded calcium indicators, and rabies targeting genes. Recombinases Addgene...Methods 11(7):763-72. PubMed (Link opens in a new window) Chuhma N, Mingote S, Yetnikoff L, Kalmbach A, ...Elife. 7. pii: e39786. PubMed (Link opens in a new window) Cummings KA, Clem RL. 2020. Prefrontal somatostatin...

...Link opens in a new window) . GenET (Genome Editing Toolkit) (Link opens in a new window) is a library of...endonuclease) Tools: Cas-OFFinder & Cas-Designer From the Kim Lab , Cas-OFFinder identifies gRNA target ...of California, Berkeley. CRISPR Software Sanger Indel Analysis ICE (Inference of CRISPR Edits) This new... datasets and allows the direct comparison of individual experiments. PubMed PMID 27404874 . MAGeCK Model-based...their efficiency. DeepSpCas9 (Link opens in a new window) : a webtool that provides models for SpCas9 gRNA...gRNA design. DeepBE (Link opens in a new window) : a webtool that provides models for base editor (BE)...BE) gRNA design. DeepPrime (Link opens in a new window) : a webtool that provides models for prime editor...

...beautiful Addgene “Find Plasmids” badge in the image below. If an abstract on PubMed has the “Find Plasmids” badge...Have you ever thought - Is there an easy way to find published plasmids? Do you spend a lot of time in...badge, you’ll know that within that article you’ll find new plasmids. Simply click on the badge to be taken...them in experiments. Best practices!). Making the find plasmids badge appear So how do you make this magical...are browsing article abstracts you should see the “Find Plasmids” box on papers that have deposited plasmids... find plasmids...

...MIcrobiology Pages The problem: Host binding proteins are hard to find Sequencing and cloning phage DNA can...evolution of their bacterial prey. Currently, receptor binding proteins (RBPs, the host recognition factors of...study. These are highly useful for the biotech industry, which is in the process of capitalizing on phage..., phages represent a reservoir of carbohydrate binding proteins waiting to be explored and exploited. ...phage genome to be probed for proteins with host-binding activity without the need for genome sequencing...sequenced genome that encode for functional cell-binding proteins in the (all-too-common) event that homology-based...coli and subsequent proteins screened for cell-binding abilities (6). This can be accomplished using a...

...! The starting point: Ligation-independent cloning Ligation-independent cloning (LIC) was first developed... personalities, SLIC (sequence- and ligation-independent cloning) would be a true rebel. Not only does...specially designed plasmids. Sequence- and ligation-independent cloning: A SLIC makeover In 2007, LIC received...His new method, named sequence- and ligation-independent cloning (SLIC), eliminated many of LIC’s constraints...PubMed. SLIC: a method for sequence- and ligation-independent cloning. Li MZ, Elledge SJ. Methods Mol Biol....guide to SLIC Resources at Addgene Ligation-Independent Cloning Protocol Gibson Assembly Blog Post Choosing...

...FAP-TAP system works. Find the Plasmids from the Publication Here Light-induced cell ablation with MG-... your model system? Turns out that with light-inducible photosensitizers and a quick zap of the proper...case). The fluorogen is not fluorescent until it binds its protein partner thus forming the full FAP-TAP...FAP-TAP complex where dL5** is the FAP. It’s job is to bind MG-2I, a photosensitizer fluorogenic dye.  MG-2I...MG-2I-dL5** He et al tested out light-induced cell ablation with MG-2I-dL5** in two different experimental...present 96 hours post treatment (see figure 2). Induced cell death was confirmed by TUNEL staining.  The...ablation efficiency, but dL5** phototoxocity is induced when expressed on the cell surface, in the cytosol...

...). Best uses Finding papers frequently cited by others. This is a great hack for finding relevant papers...Finding research papers is not particularly hard. There are millions of them. The real challenge is finding... finding relevant papers. The latest installment of the Early Career Researcher Toolbox will highlight...highlight four tools for finding journal articles related to your actual interests while also staying on top ...PubMed PubMed is the classic search engine for finding papers. It’s been around since 1996, but is getting...be summarized in 280 characters! Using Twitter to find papers  You’ll need to start following relevant ...accounts in order to turn Twitter into a paper-finding-machine. There are four general types of accounts...

...same information in our search indices in order to support different kinds of matches: exact words, partial...Fortunately, we’ve just made it easier for you to find what you need quickly.  Through the years, we have... a result, resources on the website were hard to find and many remained undiscovered.  To remedy this,...Now, let’s take a look at the least visible part behind these changes: the backend technology that powers...powers the new website search. Tackling findability from multiple angles    We assembled a cross-team team...needed to improve more than that: the general “findability” of the information on the site. Different users...users have different strategies for finding what they need, and we wanted to support all of them — searching...

...cost a fortune for a successful outcome. How do I find a coach? Start with referrals. Are there scientists...referrals. There are bad coaches out there, so finding a good one that is a style match for your needs... of transitioning a scientist from academia to industry, you might want to consult the Cheeky Scientist...We Turn Academics into Confident and Successful Industry Professionals”. This larger shop might appeal ...excellent in coaching for communication skills. Industry transition is also an area of specialty for Michelle...Resume That Gets Seen  Guide Your PhD with An Individual Development Plan Learn a Little about The Academic...Check out Our Career Page for Recent Job Openings Find Plasmids to Expedite Your Research   ...

...the ligand-binding pocket Bind specific subunit pairs, outside the ligand-binding pocket Bind specific ...antibodies that uniquely: Bind to the alpha subunit, outside the ligand-binding pocket Bind to the beta subunit... opens in a new window) based on yeast display technology (Link opens in a new window) and an optimized...Find unique recombinant antibodies targeting families of cell surface and secreted proteins from a collaboration...Protein Innovation (IPI) Collection! Here, you’ll find unique recombinant antibodies targeting families...model. Learn more about IPI (Link opens in a new window) Glypican Antibodies Glypicans are a family of ...specific subunit pairs, blocking ligand binding and receptor function Figure courtesy of IPI Browse Integrin ...

...ULBP1 UL16 binding protein 1 RAET1I ULBP2 UL16 binding protein 2 N2DL2, RAET1H ULBP3 UL16 binding protein...kinase binding protein DAP12, KARAP, PLOSL ULBP1 UL16 binding protein 1 RAET1I ULBP2 UL16 binding protein...RING11 TAPBP TAP binding protein (tapasin) NGS17, TAPA, TPN, TPSN, tapasin TAPBPL TAP binding protein-like...factor beta binding protein 1 MGC163161 LTBP2 latent transforming growth factor beta binding protein 2 ... RAB GTPase binding effector protein 1 RAB5EP, RABPT5 RABEP2 rabaptin, RAB GTPase binding effector protein...cell is primed to recognize a specific antigen, it finds and attacks pathogens or infected cells expressing...T cell, whereas T cell tolerance or anergy are induced when the TCR is engaged without co-stimulation ...

...CAG Alzheimer's Dennis Selkoe 30145 pCAX APP Swe/Ind APP CAG Alzheimer's Dennis Selkoe 30146 pCAX APP ...Aleksandra Radenovic 114194 pc DNA FRT TO- APPSwed/Ind APP CMV Alzheimer's Aleksandra Radenovic 114410 AICSDP...Huntington's Susan Lindquist 1178 p416 46Q GPD HTT GFP GPD Huntington's Susan Lindquist 1179 p416 72Q GPD...Huntington's Susan Lindquist 1180 p416 103Q GPD HTT GFP GPD Huntington's Susan Lindquist 1181 p426 25Q GPD...Huntington's Susan Lindquist 1182 p426 46Q GPD HTT GFP GPD Huntington's Susan Lindquist 1183 p426 72Q GPD...Huntington's Susan Lindquist 1184 p426 103Q GPD HTT GFP GPD Huntington's Susan Lindquist 1185 p416 25Q GAL...Huntington's Susan Lindquist 1186 p416 103Q GAL HTT GFP GAL1 Huntington's Susan Lindquist 1187 p426 25Q GAL...

... in a new window) The Malate Dehydrogenase CUREs Community (MCC) (Link opens in a new window) develops...mentor future MCC members. Find a regional hub (Link opens in a new window) and contact the hub director...opens in a new window) — Learn how to get started with a CURE, explore MDH projects, find teaching resources...expression in bacteria and cloned into an IPTG-inducible His-tag expression vector. All His-tags are cloned... cloned at the C-terminus of MDH and, if indicated on the plasmid map, a TEV cleavage site is present ...plasmid page. Use the search bar or sort buttons to find plasmids based on: Organism or species (e.g., human.... DOI: 10.24918/cs.2021.43 (Link opens in a new window) External Collaboration Results in Student Learning...

...separate the individual crRNAs. (3) The tracrRNA and Cas9 nuclease form a complex with each individual, unique...directly after where the crRNA would bind. (5) After the complex binds, Cas9 separates the double stranded...CRISPR (Clustered Regularly Interspaced Short Palindromic Repeat) sequences were initially discovered in...protospacer adjacent motif (PAM). The CRISPR complex binds to the foreign DNA and cleaves it to destroy the...foreign DNA. Protospacers are separated by short palindromic repeat sequences. (1) The CRISPR array is transcribed...pre-crRNA). (2) The pre-crRNA is processed into individual crRNAs by a special trans-activating crRNA (tracrRNA...(tracrRNA) with homology to the short palindromic repeat. The tracrRNA helps recruit RNAse III and Cas9...