...Acinetobacter spp., Aeromonas spp., Agrobacterium spp., Alcaligenes spp., Aliivibrio salmonicida, Anabaena spp., ...Gram-negative and Gram-positive  Acinetobacter calcoaceticus, Actinobacillus pleuropneumoniae, Actinomyces...Aeromonas hydrophila, Agrobacterium tumefaciens, Alcaligenes eutrophus, Azotobacter vinelandii, Brevibacterium...pSa, pR388 Gram-negative  Acinetobacter calcoaceticus, Aeromonas liquefaciens, A. salmonicida, Agrobacterium...Agrobacterium tumefaciens, A. rhizogenes, Alcaligenes eutrophus, Enterobacter sp., Erwinia amylovora, E... pBBR1 based pBBR1  Gram-negative  Alcaligenes eutrophus, Bartonella bacilliformis, Bordetella...

...doi: 10.1101/2022.08.13.503850.  iGECInano NIR calcium reporter Near-infrared (NIR) fluorescent proteins...’s lab has generated a new genetically encoded calcium indicator using small monomeric NIR fluorescent...photochemical characteristics than previous NIR calcium reporters iGECI and NIR-GECO2G, and its small size...Characterization of a Small Near-Infrared Fluorescent Calcium Indicator. Front Cell Dev Biol. 2022 Jun 29;10:...response size and reduced sensitivity to pH and calcium compared to previous lactate biosensors, and its...additional AAV serotypes of the most recent jGCaMP8 calcium sensors: pAAV-hSynapsin1-axon-jGCaMP8s-P2A-mRuby3...

...luciferase Many intracellular sensors of events like calcium release have been made using fluorescent resonance...generally consist of a responsive protein (e.g. calcium binding protein) fused to two fluorescent proteins...proteins. When the responsive protein binds to calcium it changes conformation and brings the two fluorescent... due to its bright luminescence. Intracellular Calcium Sensor Yang, J. et al. (7) wished to trigger intracellular...this optogenetic tool, they needed to create a calcium sensor with the following characteristics: No ...CalFlux VNT responds to changes in intracellular calcium and is useful for direct imaging of events in cells...

...pAAV-hSyn-DIO-EGFP   Biosensor AAV Calcium sensors Tracking calcium dynamics that govern such activities...studying neural networks. We have expanded our calcium sensor collection to include our first Flp-dependent...Several new options are also available for the calcium sensor jGCaMP7 from Janelia, including three jGCaMP7...technology, see our blog post Overview of AAV Encoded Calcium Sensors). Plasmid Serotype Name 104489 AAVrg...

...tap water. Add ethyl alcohol or acetone drop by drop onto the smear until the alcohol runs clear and immediately... one type, Streptococcus pneumoniae, while the alcohol used in the staining process bleached another, ...peptidoglycan layer, staining the cell purple. When the alcohol is added, it dehydrates the peptidoglycan layer...positive bacteria. In a gram negative bacteria, the alcohol destroys the outer lipid membrane, and the thin...

...and iGECI calcium indicator (Shemetov et al., 2021). In addition to FRET, intensiometric calcium indicators...performance, and applications of near-infrared calcium indicators in this recent review (Shcherbakova ... genetically encoded near-infrared fluorescent calcium ion indicator. Nat Methods 16, 171-174 (2019). ....A. et al. A near-infrared genetically encoded calcium indicator for in vivo imaging. Nat Biotechnol 39...

...Plasmodium falciparum genome. To put this last point into perspective, the Plasmodium falciparum genome contains...in their work editing the genome of Plasmodium falciparum (the parasite that causes malaria). I attribute...sites, scores and the statistics that go into calculating them. For this, some online tools such as Cas-OFFinder...editing in the human malaria parasite Plasmodium falciparum using the CRISPR-Cas9 system." Nature biotechnology...

...tried to find an app that could help me with calculations. This is how I came across the Gibco Cell Culture...culture and include a counter, lab timer, dilution calculator, and a couple of other nice features. This app...sequence for your enzyme of interest. Lastly, the Tm calculator helps you set up an optimal PCR depending on ...apps: Findings Lab Notebook Cell Culture Split Calculator by DRDP Restriction Enzyme Tool by Promega Material...

...fluorescence protein-based calcium sensors (jYCaMP1), variants of the calcium indicator jGCaMP7, that are...with red fluorescence protein-based calcium sensors. These new calcium sensors include syn-jYCaMP1s (slow...

...Zebrafish receptors Zebrafish antibody plasmids P. falciparum merozoite surface protein plasmids P. vivax merozoite...erythrocyte Babesia microti blood stage proteins P. falciparum sporozoite cell surface and secreted proteins...essential for erythrocyte invasion by Plasmodium falciparum. Crosnier C, Bustamante LY, Bartholdson SJ, Bei...

...SPARC to image the calcium response in the dendrites of T5 neurons by placing the calcium indicator GCaMP6f...intermediate (d), and sparse (e) labeling of the calcium indicator, GCaMP6f, in the Drosophila optic lobe...

.... Factor Xa loves calcium, but metal-chelate matrices, not so much. Maybe a calcium-independent proteinase...This allows washing away of unbound material to calculate the amount of bound ligand (after subtracting ...

...Control AAVs for serotype testing jGCaMP7c for calcium detection PSAM4 GlyR for chemogenetic inhibition...high contrast between its resting state and its calcium-bound state, which was achieved by lowering the...  AAV1  pAAV.hSynapsin.SF-iGluSnFR.S72A New calcium sensors Plasmid Serotype Name 104496  AAV1...

...might not have to do the calculation yourself as many fluorometers will calculate your sample concentration...than 1.5. Then, using the A260 reading, you can calculate the DNA concentration. Generally, A260 of 1.0 ...

... followed by a talk from Professor Karin about alcohol and obesity as driving factors for chronic inflammation...chronic hepatitis caused by hypernutrition and alcohol consumption. Chronic inflammation is a dangerous...cells that are not excitable but responsive to calcium. We’d love to expand our cancer and immunology ...

...robust, with an eNL-based calcium sensor displaying a 500% signal change upon calcium binding. Suzuki et al... to previous publications. They also developed calcium, cAMP, and ATP sensors based on Nano-lantern, showing...

...-mCherry-hPMCA2w/b Expresses a plasma membrane calcium pump hPMCA2w/b Baljit Khakh 112000-AAV1 pAAV-hSynapsin1...135420-AAV1 pAAV-syn-FLEX-jYCaMP1s Yellow protein calcium sensor expressed under human Synapsin1 promoter...pAAV-syn-FLEX-axon-jYCaMP1s Axon-targeted yellow protein calcium sensor expressed under human Synapsin1 promoter...Podgorski 135424-AAV1 pAAV-syn-jYCaMP1s Yellow protein calcium sensor expressed under human Synapsin1 promoter...

...not use CsCl are commercially available. Titer calculation (1 or 10 days) Physical titer (particle count...260nm corresponds to 1.1 x 1012 particles/mL. To calculate the particle number: OD260 reading x dilution ... days later. Plaques are counted and titer is calculated in terms of plaque forming units (pfu), taking...

...used to calculate the concentration of the sample. This concentration can then be used to calculate the viral...Maintaining a high droplet count is important for calculating the concentration of your sample. Even if you...

...Andrew Leifer’s lab utilized the worm to study calcium signaling in the entire adult brain, using a plasmid...plasmid that expresses a genetically encoded calcium sensor, GCaMP6 (Nguyen et al., 2015). If you are ...PMC5349099. Nguyen, Jeffrey P., et al. "Whole-brain calcium imaging with cellular resolution in freely behaving...