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Showing: 51 - 100 of 164 results

  1. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    ...the transfection method (i.e. polyethyleneimine, calcium phosphate or liposomal methods), the volume of ...be optimized prior to starting. We recommend calculating the ratio of the surface area of the larger vessel...

  2. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...vectors expressing GCaMP6f with this promoter. Image calcium transients in PV interneurons with AAV-S5E2-GCaMP6f...terminal providing better signal to noise for imaging calcium dynamics in fine structures. We have expanded our...

  3. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...qPCR Protocol for more details. Pro Tips When calculating the titer for your AAV, remember to account for...tool for gene expression in mammals Use the PEI calculator for planning AAV packaging transfections Find...

  5. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ...to your normal control - you can set up your calculations so that your assay output number (RQ value) ... determination of amplification of Plasmodium falciparum multidrug resistance-1 gene (pfmdr1). J Parasitol...

  6. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ... commonly used are GECIs - genetically encoded calcium indicators (e.g. GCaMP). Others include: pHluorin... of how bright is the emission. Brightness is calculated as the product of extinction coefficient and ...

  7. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...also need to know the extinction coefficient to calculate the concentration. At 280 nm, the extinction coefficient...protein standard and the concentration may be calculated using imaging software. Any bands on the gel ...

  10. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ... ligands (Figure 2). Biosensors, which include calcium and voltage sensors, are the broadest category ... vectors available in the repository. GCaMP, a calcium indicator (biosensor, by Addgene’s categorization...

  11. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ...level of antigen present in the sample and is calculated by extrapolating from the standard curve. Because...amount of antigen present in the sample and is calculated by extrapolating from the standard curve. Scientists...

  12. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...Used for neuronal/CNS expression. Modulated by calcium and calmodulin. GAL1, 10 General expression mRNA...ADH1 General expression mRNA Yeast promoter for alcohol dehydrogenase I Repressed by ethanol Full length...

  13. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    ...value cut off. This method entails arranging all calculated p values from smallest to largest and ranking...ranking them (1 being the smallest value). It calculates the cut off for significance by finding the largest...

  15. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...and tween as extended treatment can lyse cells. Alcohols, like ethanol or methanol, fix and permeabilize...first to freeze everything in place followed by alcohol permeabilization. Users should also keep their ...

  16. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...Degorce et al., 2009), measuring intracellular calcium (Miyawaki, Griesbeck, Heim, & Tsien, 1999), monitoring...which 50% FRET occurs. b Range corresponds to calculated D/A distances for observed FRET efficiencies ...

  17. 2021 Deck the Lab Winners!

    Type
    Blog Post
    ...December 10, 2021   2nd Place: The Winter and the Falcon Tube created by the team efforts of the Yit Heng...

  18. AAV Viral Preps

    Type
    Collection
    ...Archaerhodopsins Recombinases Cre, Flp, Dre Biosensors Calcium Sensors, Glutamate Sensors, Voltage Reporters Controls...

  19. Deck the Lab 2022!

    Type
    Blog Post
    ... have decorated their labs with everything from Falcon tube trees to giant paper mache Krampus performing...

  22. Fueled by Coffee at #SfN14

    Type
    Blog Post
    ... global coffee supply… My back-of-the-napkin calculations put the daily consumption here at ~ 60,000 cups...

  25. Antibody Production

    Type
    Collection
    ...generated. The final antibody concentration is calculated from the standard curve. Using ImageJ software...

  26. Editor's Choice, September 2016

    Type
    Blog Post
    ... can use in the lab. Whether you’re tired of calculating dilutions or you can’t quite remember the hazards...

  30. Sensing Neuronal Dopamine

    Type
    Blog Post
    ... animals (Patriarchi et al., 2018). Similar to calcium and glutamate sensors, the new dLight1 sensor is...

  33. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...fluorescent and luminescent siganls and a ratio is calculated (the BRET ratio), which allows for compensation...multi-gene expression and adapt it for multi-color calcium sensing (plasmids coming soon!). Takai, et al...

  35. Plasmid Cloning by PCR

    Type
    Blog Post
    ...TGGCATATCTCGAAGTACTGAGCGGCCGCTAAGCA-3’) into this calculator we get a final Reverse Primer sequence of 5’-...

  37. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...acceptor molecules and can therefore be used to calculate the distances between the donor and acceptor. ...

  48. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ...doi.org/10.1523/ENEURO.0171-19.2019Jendryka M, Palchaudhuri M, Ursu D, van der Veen B, Liss B, Kätzel D,...

  49. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ..., which are resistant to ethanol and isopropyl alcohol, 0.5% peracetic acid, 10% bleach, or 1% iodine ...

  50. Hot Plasmids February 2024

    Type
    Blog Post
    ...doi.org/10.21203/rs.3.rs-3188559/v1.  Neurogenetic alchemy: CAP-Mac unleashes the secrets of primate brains...
Showing: 51 - 100 of 164 results