Developing a cold tolerant E. coli using specialized chaperones
Type
Blog Post
...transformed mesophilic, for example E. coli, carrying a plasmid expressing a protein of interest). Mesophilic microbes...granules.
We overcame this problem by engineering a plasmid which contained cold tolerant chaperones (the Cpn...pMBP-LbCas12a and pC013-Twinstrep-SUMO-huLwCas13a plasmids from Addgene, which would potentially allow a ...biology blog posts
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