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We narrowed to 893 results for: plasmid

Showing: 361 - 380 of 893 results

  1. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

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    Blog Post
    ...to elute and store your plasmid DNA Learn about the different grades of plasmid DNA preps Resources on...studies and is not used as much for the standard plasmid prep. Diphenylamine method Another absorbance based...Addgene.org Visit the Molecular Biology Reference Find plasmids for your research Browse protocols for molecular...

  2. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type
    Blog Post
    ...The principle of Tetbow In modern neuroscience, plasmid or viral vector-mediated gene delivery is more ... use Tetbow, you can introduce the four Tetbow plasmids (Addgene #104102 - #104105) using in utero electroporation...Tips and troubleshooting your Tetbow experiment Plasmid concentrations and AAV titer - The XFP genes have...achieve the highest color variations. Adjust the plasmid concentrations or AAV titer for the best results...

  3. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    ...been cloned into plasmids. Because their sequences are known and encoded on a plasmid, they can be easily...recombinant antibodies. Recombinant antibodies are plasmid-based and are created synthetically or derived ...regions of a specific antibody are cloned into a plasmid backbone containing all constant regions, just ...

  4. How Do I Place an Addgene Order Using a PO Number?

    Type
    Blog Post
    ...of information about how to deposit plasmids, store your plasmids or viral items, technical support, and...your favorite store is easy. Ordering genes on plasmids online is a little trickier -- but at Addgene,...

  5. Why Do I Need an MTA Anyway?

    Type
    Blog Post
    ...for material transfers. When Addgene entered the plasmid sharing space, we heard over and over again that...with the UBMTA result in quick approvals, so your plasmid requests can get to your lab faster. For Everybody...reference pages. So go ahead and use Addgene plasmids in your experiments. You know that they’re properly...

  6. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    ...transcription from the accessory plasmid (AP) encoding phage gene III. The accessory plasmid library contained all...PAM flexibility. In this system, the mutagenesis plasmid (MP6) permits quick, large-scale mutation of the...doi: 10.1038/nature26155 PubMed PMID: 29512652 Plasmids from this paper are available at Addgene.  Additional...Addgene.org Check out our CRISPR Guide Find CRISPR Plasmids for Mammalian Systems Learn about CRISPR History...

  7. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    ...Gonçalves and Ventura labs’ plasmids, including protocols, check out the plasmids at Addgene: pAdSh.PGK.Cas9...looking for a broader range of CRISPRs plasmid tools, find more plasmids, CRISPR technology guides, FAQs, and...description of their experimental protocol. The three plasmids which have been deposited to Addgene are: pAdSh.PGK.Cas9...

  8. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    ...generation of mutant cell lines. Further, for each plasmid in the kit, the transcript most commonly found ...initiative to curate our collection of cancer-related plasmids such that each is linked to a known major pathway...pathway. Now, using our new Cancer Research Plasmids and Resources page, investigators may select a pathway...investigator to a list of all available Addgene plasmids he or she can use to study it (as seen in the ...Visit our Cancer Collection Pages Deposit Your Plasmids for Studying Cancer Browse Our Other Collections...

  9. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ...: 1262. PMID: 23232392 PMCID: PMC3535334 Find plasmids from this paper at Addgene. Takai, Akira, et ...4352-6. PMID: 25831507. PMCID: PMC4394297 Find plasmids from this paper at Addgene. Suzuki, Kazushi, ... 13718. PMID: 27966527 PMCID: PMC5171807 Find plasmids from this paper at Addgene. Additional Resources...All Our Fluorescent Protein Resources Find FRET Plasmids Find Empty Backbones with Fluorescent Protein ...

  10. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...specific mutations in your plasmids Use REPLACER Mutagenesis to mutate your plasmid Resources on Addgene.... targeted mutagenesis experiments? Find EvolvR plasmids at Addgene! References Gaj, Thomas, and Pablo...Browse our CRISPR guide pages Find bacterial CRISPR plasmids ...

  11. Multiplexed Overexpression of Regulatory Factors (MORF) collection

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    Blog Post
    ...Addgene.org Plasmid Pooled Libraries Pooled library amplification Zhang Lab CRISPR Plasmids The Human TFome...controls. Alternatively, you can order individual plasmids from the library to further investigate specific... of the collection, we’re releasing individual plasmids in batches. If there’s something you’d like that...

  13. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

    Type
    Blog Post
    ...antibiotic selectable markers by the destination plasmid. Antibiotic selection allowed for high throughput...accurate assemblies was achieved by the sequencing of plasmids isolated from blue or white colonies. Sequencing...occasional uncut or cut/re-ligated pGGA destination plasmids. A final validation of the 5-, 12- or 24-fragment...'s work on ligase efficiency Learn about other plasmid cloning techniques Resources on Addgene.org Find... Find basic molecular biology protocols Find plasmid cloning protocols Check out our molecular biology...

  14. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ... great collection of ready-to-use rAbs and rAb plasmids!). Since recombinant antibodies conveniently allow...antibodies can be produced through a single or dual-plasmid transfection, depending on whether the heavy and...and light chain genes are contained to one plasmid or if they’ve been separated onto two. You’ll first...first transfect your cells with your plasmid(s), and then wait 7-14 days (in mammalian cells) to collect ...on the Addgene blog Introduction to Antibodies Plasmid-based Recombinant Antibodies Polyclonal Antibodies...

  15. Editor's Choice, September 2016

    Type
    Blog Post
    ... Tips for Writing a Good Cover Letter Multiple Plasmids at a Low Price: Kits from Addgene Savvy Advocates...Kinome Libraries Available: Pooled and Individual Plasmid Formats Better Dyeing Through Chemistry & Small...Addgene supporters who spoke with me and deposited plasmids on this trip. Here’s to hoping that October is...

  16. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    ...mammalian cells is transiently transfected with a plasmid containing a fluorescent protein (fusion) there...dish. This is caused by the stochastic nature of plasmid uptake by cells. To correct for this variation,...Goedhart et al, 2012, Bindels et al, 2017). The plasmid for co-expressing mTurquoise2 with mVenus as a ...Find Empty Backbones with Fluorescent Tags Find Plasmids for Subcellular Localization Browse the entire...

  17. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    ...transformed mesophilic, for example E. coli, carrying a plasmid expressing a protein of interest). Mesophilic microbes...granules. We overcame this problem by engineering a plasmid which contained cold tolerant chaperones (the Cpn...pMBP-LbCas12a and pC013-Twinstrep-SUMO-huLwCas13a plasmids from Addgene, which would potentially allow a ...biology blog posts Resources on Addgene.org Find plasmids for your synthetic biology experiment Read our...

  18. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

    Type
    Blog Post
    ...that Addgene provides is an open repository of plasmids and other necessary information that’s required...the collection, storage, and distribution of the plasmids and their ability to identify and fulfill a need... helping the scientific community manage those plasmids so scientists aren’t bogged down with distributing...continuing to look at providing other things besides plasmids to the scientific community through Addgene’s ...

  19. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ...exact Cas9 plasmid used by Jinek et al. 2012, or browsing for the latest AsCas12a plasmids, Addgene and...find a link to search Addgene's collection for plasmids containing that enzyme, and embedded in the References...lists on every page you can find direct links to plasmids from the article if they're available through ...

  20. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    ...received the first CRISPR plasmid deposit. Now we have over 8,000 CRISPR plasmids in the repository that ...
Showing: 361 - 380 of 893 results