... which he was a fellow at the Bauer Center for Genomics Research at Harvard University.       ...

...Gordon Fishell 99120-AAV1 pAAV-ihSyn1-tTA An AAV genome that expresses tTA from the hSyn1 promoter that...Gradinaru 99121-AAV1 pAAV-ihSyn1-DIO-tTA An AAV genome with Cre-dependent expression of the tTA from the...Gradinaru 99130-AAV1 pAAV-mDlx-NLS-mRuby2 An AAV genome that expresses nuclear localized mRuby2 from the...Gradinaru 99130-AAV2 pAAV-mDlx-NLS-mRuby2 An AAV genome that expresses nuclear localized mRuby2 from the...Gradinaru 99130-AAV8 pAAV-mDlx-NLS-mRuby2 An AAV genome that expresses nuclear localized mRuby2 from the...Gradinaru 99130-AAV9 pAAV-mDlx-NLS-mRuby2 An AAV genome that expresses nuclear localized mRuby2 from the...Gradinaru 99130-AAVrg pAAV-mDlx-NLS-mRuby2 An AAV genome that expresses nuclear localized mRuby2 from the...

...Collections Structural Genomics Consortium Plasmids Browse All SGC Plasmids Structural Genomics Consortium Plasmids...Browse plasmids from the Structural Genomics Consortium (SGC) for 3D protein structure studies Plasmid...

... Genome Engineering CRISPR Joung Lab CRISPR Plasmids Joung...endonucleases that can be used to perform targeted genome editing. RGNs consist of two components: a short...variable nts at its 5’ end to base pair with a target genomic DNA sequence and a nuclease (e.g.—the Cas9 endonuclease...www.crispr-cas.org . Users are also encouraged to join the Genome Engineering newsgroup here . Links to additional...

...bacterial genome editing is not always straightforward. Read our CRISPR Methods for Bacterial Genome Engineering...using CRISPR in your lab, specific CRISPR tools for genome editing in bacteria, and more. Synthetic Biology...range of organisms. Find CRISPR tools for use in genome editing of bacteria. CRISPathBrick Multiplex Plasmid...opens in a new window) JGI Integrated Microbial Genomes and Microbiomes (IMG/M) (Link opens in a new window...Program (Link opens in a new window) Saccharomyces Genome Database (SGD) (Link opens in a new window) Return...

...Plasmid Collections Structural Genomics Consortium Plasmids Structural Genomics Consortium Plasmids You may...Plasmids from the Structural Genomics Consortium (SCG), which are primarily used for determining protein...like... David Waugh Lab plasmids The Structural Genomics Consortium is a not-for-profit organization that...

...sequencing of the Viral Genome Next-generation sequencing is performed on viral genomes isolated from the final...confirm the identity and integrity of the viral genome and the absence of unexpected DNA contaminants....pAAV-Ef1a-mCherry-IRES-Cre (55634-AAVrg) alone at 1.7E6 viral genomes (vg)/cell, pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2] ...Electron Microscopy The ratio of empty to full (i.e., genome containing) AAV particles within representative...of a lentiviral vector, incubated for 72 h, and genomic DNA is extracted. Primers and probes targeting ...harvesting cells several days later, and isolating genomic DNA. This DNA is then used as a template to amplify...

...exactly match your genomic target? Variation between a given gRNA sequence and your genomic target may reduce...Plasmids: gRNAs Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...gRNA plasmids designed to target various genes or genomic regions. It is highly recommended that you determine...

...gmail.com We have found that a variety of Cas9-mediated genome modifications can be efficiently generated in Drosophila... injection approach, stable lines with targeted genome alterations can be generated within a month. phsp70...invertebrate species. These plasmids are described in: Genome engineering of Drosophila with the CRISPR RNA-guided...homology-directed repair to replace genes or other genomic sequence with an attP docking site. Contains the...

...loss of function alleles. To introduce specific genomic changes, researchers use ssDNA or dsDNA repair ... Publication Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...the nickase to the target site by homology to a genomic DNA locus and encodes a primer binding site and...Cas9 (dCas9) can be used to purify a region of genomic DNA and its associated proteins, RNA, and DNA. ...fluorescent protein (FP) can help visualize specific genomic loci using fluorescent microscopy in living cells...sequence to direct the dCas9-FP fusion to a specific genomic sequence - potential target locations can include...

...inhibits over 18,000 human genes and is used for genome-wide inhibition screening. Find the Dolcetto lentiviral...

...developed heat shock-inducible Cre and Cas9 for easy genome engineering in species like C. elegans and Drosophila...

...AAV preparations were always normalized to their genomic titers). Examples include the use of exo-AAV1 for...

...randomly integrating viral vectors, CRISPR/Cas9 genome editing allows targeted engineering of the TCR ...

.../ .   References 1. Cong, Le, et al. "Multiplex genome engineering using CRISPR/Cas systems." Science ...

...methylation also plays an integral role in regulating genome replication, repairing mismatched basepairs or ...

...plasmids, with the response regulator encoded in the genome. To make the system less bulky and easier to use...

...openness” of the lab (i.e projects like the Personal Genome Project and open source software, hardware, wetware...

...Cas9 is becoming an ever more ubiquitous tool for genome engineering, and studying its structure can help.... Structural biology of CRISPR–Cas immunity and genome editing enzymes. Nature Reviews Microbiology, 20...

...These viruses also integrate stably into the host genome, enabling long term transgene expression. There...because this virus integrates randomly into the host genome, it has the potential for insertional mutagensis...conjunction with the plasmid that encodes the viral genome (typically called the transfer plasmid ). This ...Popular Lentiviral Transfer Plasmids The lentiviral genome (i.e., the genetic material enclosed in the viral...lentivirus is used for research, it is the lentiviral genome that encodes genetic material that the researcher... wants delivered to specific target cells. This genome is encoded by plasmids called "transfer plasmids...