...tumorigenesis The conference opened with a very interesting presentation by Professor Guido Kroemer from ...presentation, starvation or the use of caloric restriction mimetics trigger autophagy and, in a mouse model...References: 1. Pietrocola, Federico, et al. "Caloric restriction mimetics enhance anticancer immunosurveillance...

... indels for “functional knockout” of a gene of interest is one of the most common applications of precision...important difference (Figure 3). If you are interested in harnessing the power of digital PCR screening...Postdoctoral Fellow at the University of Alberta. He is interested in everything molecular biology. Follow him on...

...the bacteria can easily uptake your plasmid of interest. Scientists can also use electroporation, the ...scientists to introduce different DNA sequences of interest into a bacterial cell or a host’s genome. To do...create both transient cell lines, where a gene of interest is expressed but not integrated into the genome...

...first developed in the 1990s. While traditional restriction enzyme cloning uses short sticky ends to insert.... To start the SLIC cloning process, a gene of interest is PCR amplified to add 5′ and 3′ homology regions...multiple fragments without the need for specific restriction sites or DNA ligase! This post was originally...

...efficiency. However, gamma-retrovirus infection is restricted to dividing cells only, so is not suitable for...can target the virus to specific cell types of interest. This is a particularly nice feature as organ-...have an idea which ones could be helpful to you. Interested in learning more about a specific virus? We have...

...been hampered by the need to tag each protein of interest and, for in vivo work, generate a dedicated transgenic...GFP-nanobody rather than a specific protein of interest. This fusion protein can then be co-expressed ...expressing a GFP-tagged version of a protein of interest. The binding of the GFP nanobody to its target...

...activated B cell that produces the antibody of interest with a myeloma cell. This RNA was then used to...Constructs were then verified by both diagnostic restriction digest and colony PCR.   Fig. 1: (A) ... found that the recognition sequence for the restriction enzyme BciVI was present in the aberrant light...

...Researchers express genes of interest from plasmids in order to study gene function or to engineer cells...fluctuations due to copy number by introducing genes of interest into the chromosome. But this has its own set ...thereby intensifying repression of the gene of interest and keeping its expression level constant. In ...

...Health Science Center at Houston. Prakash became interested in studying TS when he was studying thoracic ... Dr. Carolyn Bondy, and Dr. Angela Lin got me interested in TS when I was a newly independent faculty ...channels in the brain.  “I have a long standing interest in the biology of GPCRs and G proteins, with an...

...doing after the injunction? We are particularly interested in whether institutions planning to raise salaries...our FOIA requests, we are starting to see very interesting data on how much postdocs are actually getting...Executive Director of Future of Research. He is interested in advocating for and with junior scientists,...

...fail. Our laboratory manager would definitely be interested to see which part of her procedures are the most...getting up and running and more time working on interesting biotechnology code.There are valid technical ...Shamloo is a Software Engineer at Addgene and is interested in writing software tools to help scientists....

...them a quick scan for interesting key words and download what seems interesting or relevant. Harness the...keeping up with the literature in your field of interest. Set aside some time each day or week to at least...

...When this interaction occurs, BirA* activity is restored, and nearby proteins are labeled with biotin. ...difficult to detect proteins with short half-lives. Interested in using Split-BioID with your research? Plasmids...

...in plants to conditionally deplete proteins of interest in C. elegans (Zhang et al., 2015). In this system...spatially and temporally knock-down proteins of interest.  Plasmids for cGAL and split cGAL systems The...halves of cGAL can drive expression of your gene of interest.  SapTrap Tool Kit for CRISPR Cas9 edits At Addgene...

...which may be of particular interest to MoClo users!). If you’re interested in complex Golden Gate Assembly...overhang set by uploading the set with the Type IIS restriction enzyme and thermocycler protocol. It will also...

...potentially signaling a rough road ahead for students interested in leading the process despite the recent advance...students will pursue this newly available avenue. Interestingly, both of these changes share a common thread...understand human disease.  Outside the lab, he is interested in communicating science and in the intersection...

...integrity of your virus, perform a diagnostic restriction enzyme digest and compare it to some reliable... use the virus immediately upon harvesting. Interestingly, freeze-thawing host cells immediately prior...24003179. PubMed Central PMCID: PMC3760253. 2. Rohwer, Forest. "Global phage diversity." Cell 113.2 (2003): 141...

...and type of target sites that flox your genes of interest, a powerful FLEx switch can be created for your... considered "Cre-On" meaning that your gene of interest starts in the inverse/antisense "off" position...can be employed for "Cre-off" (i.e. your gene of interest starts in the sense orientation and Cre is used...

...chance as entrepreneurs. To make things more interesting, we are working in a new technical discipline...things we could do for work, and they weren’t restricted to science jobs. Keira’s an optimist, I’m a realist...doing makes sense. I can support that.” That was interesting. If people could be against GMOs as food but ...

...with your transfer plasmid, carrying your gene of interest, and the two helper plasmids, commonly called ...Addgene AAV Titration by qPCR Protocol. If you’re interested in the ddPCR titering process, which is what ...non-genome-containing (empty) capsids, thus leading to an overestimate of transducible units in the virus preparation...