...your supervisor’s expectations Every supervisor has their own management style. A hands-on supervisor...agree with all of your ideas, but he or she probably has reasons for it. Do not take criticism personally ... of Finish Your Thesis, an online community that has supported thousands of graduate students since 2009...people: Hostile-aggressives, who are usually antagonistic and impolite, Complainers, who spend most ...

...establish for this to happen.Using red light has a second advantage: red light penetrates deeper into biological... to its unrivaled contrast, fluorescence imaging has emerged as the dominant light microscopy contrast...blue to excite a molecule of GFP. Because red light has approximately half the energy of blue light, two ...imaging depths closer to 1 mm. Traditionally, 2-photon has been a very slow imaging technique but recent advances... modern explosion in light microscopy techniques has provided biologists with a host of tools and opened...

...deactivate specific genes are needed. Targeted mutation has been demonstrated in zebrafish using zinc-finger ... in all cells of the developing zebrafish embryo has been mapped using single cell RNA sequencing approaches...1990’s, two revolutionary large-scale random mutagenesis screens produced ~1,500 zebrafish mutants that...to study development and disease. Additional mutagenesis projects have since been initiated and today ...gene deactivation tools for zebrafish Random mutagenesis screens provided a major toehold into understanding... the one-eyed pinhead mutant from the random mutagenesis screens. Microscopy-based lineage tracing can...profiled 38,731 cells from 694 embryos over multiple stages and reconstructed the patterns of gene activity...

...keynote speaker, Dr. Henry Bourne (shown in photo), has been speaking out and writing about issues in our...the same time, he pointed out, academic research has some big problems resulting from past practices and...alternate training needs to be provided to take advantage of all the possible science careers. She advocated...

...HDR, NHEJ is active throughout the cell cycle and has a higher capacity for repair, as there is no requirement...ends interfere with that step. Accordingly, NHEJ has a vast toolbox of processing factors, including polymerases...Wyatt and Dale Ramsden, UNC at Chapel Hill. One advantage to using the CRISPR/Cas system for genome engineering...Persistence of a Chromosome Break Diminishes Its Mutagenic Potential. PLoS Genet 5:e1000683 . https://doi.org...

...research project can take several years to complete and has multiple benchmarks. Think about what your personal...Have a look at what others have done recently that has been a big hit, and especially look at the new tools... as a stepping stone into the next professional stage of your career by connecting you with people of ...

... genes of interest into the chromosome. But this has its own set of problems. Rapidly dividing cells initiate...rounds of replication are initiated before the cell has divided. This means that genes located near the origin...growing slowly or quickly Growth media and growth stage can both affect gene copy number. Moreover, gene...References 1. Segall-Shapiro, Thomas H., Eduardo D. Sontag, and Christopher A. Voigt. "Engineered promoters...

... human suffering and death. Doing research right has the potential to save lives and money. A 2014 analysis...physiologic or pathologic processes. Much progress has been made in this area in recent years, and you can...important point, however, is that cost at this early stage of research is minimal compared to that of human...trials. And, as Janine Clayton points out, late-stage clinical studies that draw from a body of research...

...often used, the CRISPR approach based on HDR has the advantage of being a potentially universal treatment... to edit genes in several biological systems. It has been used for instance in inherited disorders such...-transformed cells yielded rates of targeted mutagenesis similar to those achieved by isogenic AdVs encoding...gRNAS1, and pAdSh.U6.gRNAGFP. Gonçalves says that advantages of AdVs include their episomal nature and very...CRISPR/Cas9 nuclease complexes induces targeted mutagenesis in a diverse array of human cells. Sci Rep 4:...

...interference (CRISPRi) has been limited to lab-adapted bacteria because it has been challenging to introduce...the bacterial Tn7 transposon system. This system has two parts: a plasmid expressing the Tn7 transposition...for CRISPRi transfer Mobile-CRISPRi offers two advantages over existing bacterial CRISPRi methods. First...

...learn about antibodies? Our new antibodies animation has all the information! Link in bio. #STEM #antibodies...our videos will also be shared on our @addgene Instagram account, or you can watch the full animation on...

...developed for use in mammalian cells, this system has also been implemented in a variety of other organisms...Drosophila cells. Work by Wei et al. from Kang Shen’s lab has also shown that the Q system is also functional in...elegans. Subsequent work by the Luo and Potter labs has refined the Q system and introduced the second generation...multiple genes in synthetic systems. Recent work has even used all 3 in a single modular vector for ease...GAL4 gene downstream of a tissue- or developmental stage-specific promoter and design a UAS reporter construct...

...buffer such as TE. You will now have plasmid DNA that has been purified away from the bacterial proteins and...DNA are denatured. Pro-Tip Do not vortex at this stage or the genomic DNA will become sheared and will ...

... its introduction in 2015 by the Boyden Lab, ExM has been steadily growing in popularity with an abundance...target concentration during expansion. One group has found that using Fab fragment secondaries, rather...experimental approaches. Since it arrived on the scene, ExM has helped researchers gain a deeper understanding of...expansion.  In some cases, labels are added after this stage.  Step 4: Expand! (Typically by just adding water...

...months, COVID-19 has had a tremendous impact on our world -- as of June 4, 2020, COVID-19 has caused an estimated...contains requests from 5/22/14 to 5/19/20). Each paper has between 1–32 plasmids and a total of 2–2,590 requests... 5: Inferred effects. Values are the inferred percentage of normal activity (the average rate of unique...

...plasmid we have at Addgene. Each sample at Addgene has four layers of location which can all be extrapolated...hold up to 96 tubes (see image below). Each plate has numbers and letters to give coordinates, from A1 ...replicate sample. The transformation After the DNA has been received and stored, it is put into E. coli ...and prepare fresh media with antibiotics at each stage. The barcodes and LIMS help us track each sample...

...product packaging) for free! It has to be from their company and it has to be packaging (no coated tissue...box, flip the flaps of the box so the pre-paid postage stamp is on the outside of the container, and drop...

...include early stage drug discovery and pre-clinical development for First-In-Man studies. Kurt has been running...Avengers This year Addgene, where my wife Lianna has made her career as Senior Director of Biology, is...

... (SB) transposon system is one such system which has been designed to perform gene transfer in vertebrates...defective transposase found in fish. This transposase has been discovered in the genomes of many different ... both of these cases, transposons combine the advantages of viruses and naked DNA while eliminating some...

... long is the average PhD in this department? How has the department worked with students to maintain these... for everyone involved in the process. COVID-19 has changed many aspects of academia, and the PhD application...traveling for campus visits. This is definitely an advantage of surviving in a virtual world over the course...and the in-person portion got canceled, taking advantage of virtual meetings can still be a great opportunity...exception. However, using the virtual world to your advantage can strengthen your application and help you decide...