...two phosphatases, PTPN1 and PTPN2, which are of interest as cancer immunotherapy targets but are embryonic...s laboratory at Harvard Medical School and is interested in improving CD8+ T cell responses to cancer....generation of exhausted CD8+ T cell subpopulations and restrains tumor immunity. Nature Immunology 20, 1335–1347...

...expression. Cultures are then lysed and the protein of interest is purified using an affinity tag. Additional ...Determining the best way to express one’s protein of interest can save a lot of time later. Protein purification...C-terminal. Each set of plasmids uses the same restriction enzymes for cloning (see schematic below), enabling...

... to on Twitter? Prior to writing this post, my interest in Twitter was fleeting. I’ve had an account for...Unfortunately, it never clicked for me. Recently my interest was renewed in part due to FOMO  but mostly because... scientist? If you don’t use Twitter, are you interested in getting started? Reply in the comments below...

...With many interesting articles coming out in a myriad of journals every week, it can sometimes be difficult...plasmids and publications relevant to your research interests. We do this by promoting citation, supporting ...encourage you to fill out this form if you’re ever interested in writing your own blog post about a plasmid...

...to determine which job boards list positions interesting to you. Many job postings will result in hundreds...address how you are qualified and why you are interested specifically in the position. We frequently receive...cover letters. It is also important to express interest, even excitement, in a position. Everyone prefers...

...somewhat unusual things. For example, I am not that interested in meeting movie stars, but when I met the astronaut...a scientist up there, but the panelists were interesting enough to make up for the somewhat naïve questions...their seminal, cooperative paper in Science.  Interestingly, this project started with their labs studying...

...figures of interest along with the article metadata, legend and user created notes on a “Pinterest for scientists... scientific literature? Very often the data of interest are in 1 or 2 figures from one paper and then ...

... Short segment of DNA which contains several restriction sites allowing for the easy insertion of DNA....“vectors” or “constructs.” To insert a gene of interest into a vector, scientists may utilize one of a... a variety of cloning methods (restriction enzyme, ligation independent, Gateway, Gibson, etc). The cloning...

...2008 the Quake Lab at Stanford University became interested in exploring biological dark matter – large tracts...like YNP thermal sites - are also of practical interest since they’ve previously yielded valuable enzymes...bacterial RNA-Seq. This enriches for transcripts of interest without an increase in the number of steps in ...

...NEBCutter2: For finding restriction enzyme cut sites Webcutter 2.0: For finding restriction enzyme cut sites ... different types of cloning methods, such as restriction enzyme cloning, Gibson assembly, or In-Fusion...

...plasmid that produces biotin ligase and a protein of interest fused to maltose binding protein (MBP, New England...-Avi-His/BirA to add biotin to your protein of interest Grow fusion protein plasmid in NEB T7 SHuffle... Vanderbilt Eye Institute and is particularly interested in cell signaling.     Additional Resources on...

...several excellent guest posts from our depositors. Interested in writing your own post for the Addgene Blog...(AAVs) are commonly used to deliver a genes of interest to a variety of cell types in-vivo. These are ...customizable functions to your DNA sequence of interest. Potential applications of the system include ...

...overcome. Conflict, and its inherent tension, is interesting. It’s what drives a story forward - and this ... the pieces that make you think, “Oh, that’s interesting! How did you solve that problem?” Those are the...your audience in with something that sparks their interest and makes them want to know more. This could be...

...collection, you'll likely find some proteins of interest for which you have several antibodies in stock...another, or if you’re looking for a protein of interest while it’s in a transition state or specific configuration... to validate that it recognizes the protein of interest in specific configurations or conditions. In those...

...easiest and clearest ways to determine if your sample has recombined. Choose a restriction enzyme that...one resulting plasmid carries the transgene of interest (aka the viral genome) and the other contains ...

...clone 500-700 bp homology arms for your gene of interest into the FP-SEC vector using Gibson assembly. ...as well as a promoter-FP fusion for the gene of interest. L1/L2 larvae from these strains are then heat...applications, SEC selection could replace other, more restrictive C. elegans selectable marker systems. This novel...

...identified some fly stocks and other reagents of interest, the next question to ask is what to do with them...fly line expressing Gal4 in your tissue type of interest. You can quickly mate your UAS-YFG line with a...different tissue types! Although putting a gene of interest directly downstream of a tissue-specific promoter...

...site of interest. A gRNA is used to direct the PolI3M-nCas9 complex to a DNA site of interest, which nCas9...introduce diversification by targeting a site of interest with dCas9 fused to the Activation Induced Cytidine...

...all viral genes are flanked by EcoRI and BamHI restriction sites - so cloning projects should be fun and...researchers. We have been excited to see so much interest, because it means more scientists will be working...Communications Team at QBI UCSF. They are particularly interested in facilitating collaborative science, and making...

...determine if the fluorescent proteins you are interested in are compatible with your microscope optics...related protein, if you know that your protein of interest has a similar spectrum. For example, here's a ...need to try several proteins in your system of interest. Finally, in this set of proteins the green and...